Plasma acid reproduces oxidative and nitrosative stress in bladder tissue in vitro: experimental study

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Abstract

Aim. To analyze some effects of plasma acid in vitro on the bladder tissue obtained from laboratory animals and to evaluate the possibility of its application for in vitro modeling of IC/BPS. Materials and methods. The tissue samples of the bladder wall were obtained from female Wistar rats aged 3 months (n=16, weighing 180-200 g). The tissues were processed for 1 hour in the plasma acid prepared by spark discharge of water for injection in air. The immunohistochemical study of obtained samples was performed. Results. The changes in the expression profile of bladder epithelial cells under the action of plasma acid in vitro were found indicating the development of oxidative, nitrosative and dicarbonyl stress, impaired expression of NADPH oxidase DUOX2 and VEGF, and a decrease in cell proliferative activity, which, in general, corresponds to the main mechanisms of urothelial alterations specific for the IC/BPS. Conclusion. The revealed effects of plasma acid on bladder epithelial cells confirm the possibility of using it as an inducer of urothelial cell damage typical for IC/BPS in the in vitro models.

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About the authors

E. A Alekseeva

FGBOU VO Krasnoyarsk State Medical University. Prof. V.F. Voino-Yasenetsky Ministry of Health of Russia

Email: vohminak@maii.ru
Ph.D., associate Professor at the Department of Urology, Andrology and Sexology Krasnoyarsk, Russia

M. A Firsov

FGBOU VO Krasnoyarsk State Medical University. Prof. V.F. Voino-Yasenetsky Ministry of Health of Russia

Email: firsma@mail.ru
Ph.D., Head of the Department of Urology, Andrology and Sexology Krasnoyarsk, Russia

N. A Malinovskaya

FGBOU VO Krasnoyarsk State Medical University. Prof. V.F. Voino-Yasenetsky Ministry of Health of Russia

Email: malinovskaya-na@mail.ru
Ph.D., MD, professor, Head of the Department of Biological Chemistry with courses in Medical, Pharmaceutical and Toxicological Chemistry Krasnoyarsk, Russia

A. B Salmina

FGBOU VO Krasnoyarsk State Medical University. Prof. V.F. Voino-Yasenetsky Ministry of Health of Russia; FGBNU Research Center of Neurology

Email: allasalmina@mail.ru
Ph.D., MD, professor, Chief Researcher of Scientific and Research Institute of Molecular Medicine and Pathobio chemistry; Chief Researcher of Laboratory of Neurobiology and Tissue Engineering Krasnoyarsk, Russia; Moscow, Russia

V. V Salmin

FGBOU VO Krasnoyarsk State Medical University. Prof. V.F. Voino-Yasenetsky Ministry of Health of Russia

Ph.D., MD, Head of the Department of Medical and Biological Physics Krasnoyarsk, Russia

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