Effects of magnesium salts on the course of experimental calcium-oxalate urolithiasis


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Experimental urolithiasis was induced in 80 white non-inbred male rats by adding 0.75% ethylene glycol and 2% ammonium chloride to drinking water by Fan et al. After significant differences in crystalluria, oxaluria and urine pH were achieved in hyperoxaluric rats vs controls one, hyperoxaluric rats were given magnesium (Mg) salts Mg chloride, Mg L-aspartate either alone or in combination with pyridoxine hydrochloride (B6) in comparison with Mg sulfate and magne B6 (mg lactate in combination with B6) in a dose of 50 mg of elementary Mg per 1 kg of body weight. All the rats were fed with Mg-adequate diet containing 0.84 g of Mg oxide (0.5 g of elementary Mg per kg of diet). Calcium-oxalate urolithiasis has developed in rats taking ethylene glycol and ammonium chloride for 28 days. An urinary oxalates levels increased threefold, oxalate/creatinine - fourfold. Calcium oxalate crystals were detected in the urine of rats drinking solution of ethylene glycol and ammonium chloride, pH decreased by 20%, fractional excretion (FE) of Mg increased by 60%, FE of phosphate - by 58.2%, FE of calcium - by 95.8%, creatinine clearance lowered by 39.2% in comparison with intact group. Magnesium salts administration resulted in reduction of urine oxalates, crystalluria, phosphate excretion, Ca/Mg and oxalate/creatinine ratios, increased urine pH and creatinine clearance. Mg L-aspartate in combination with vitamin B6 appeared the most effective salt and significantly more effective than magnesium sulfate.

References

  1. Лопаткин Н. А. Состояние и перспективы развития урологической помощи в Российской Федерации / Н. А. Лопаткин, А. Г. Мартов // Материалы X Российского съезда урологов. - М., 2002. Официальный сайт Российского общества урологов. - [Электронный ресурс]. - Режим доступа: http://www.uroline.ru/society/plenum/sjesd1.php3
  2. Hammarsten G. Calcium oxalate and its solubility in the presence of inorganic salts with special reference to the occurrence of oxalate. C. R. Lab. Carsberg 1929; 17: 1-81.
  3. Evans R. A., Forbes M. A., Sutton R. A. L., Watson L. Urinary excretion of calcium and magnesium in patients with calcium-containing renal stones. Lancet 1967; 2: 958-961.
  4. Kohri K., Garside J., Blacklock N. J. The role of magnesium in calcium oxalate urolithiasis. Br. J. Urol. 1988; 61: 107.
  5. Li M. K., Blacklock N. J., Garside J. Effects of magnesium on calcium oxalate crystallization. J. Urol. (Baltimore) 1985; 133: 123.
  6. Bizaz S., Felix R., Neuman W. R., Fleisch H. Quantitative determination of inhibitors of calcium phosphate precipitation in whole urine. Min. Electrolyte Metab. 1978; 1: 174.
  7. Khan S. R., Shevock P. N., Hackett R. L. Magnesium oxide administration and prevention of calcium oxalate nephrolithiasis. J. Urol. (Baltimore) 1993; 149: 412-416.
  8. Su C.-J., Shevock P. N., Khan S. R., Hackett R. L. Effect of magnesium on calcium oxalate nephrolithiasis. J. Urol. (Baltimore) 1991; 145: 1092-1095.
  9. Essen T., Akinci M., Aytekin Y. et al. The effects of magnesium, citrate, pyrophosphate, and pyridoxine on the lithogenic substance excretion in hyperoxalic rats. In: 2-nd European symposium on stone disease. Basel; 1990.
  10. Essen T., Akinci M., Tellaloglu S., Kocak T. Role of inhibitor deficiency in urolithiasis. Deficiency grade-adjusted and intermittent augmentation therapy for magnesium and citrate deficiency. Eur. Urol. 1991; 19: 244.
  11. Albuqueqaue P. F., Tuma M. Investigation on urolithiasis. Studies on oxalate. J. Urol. (Baltimore) 1962; 87: 504.
  12. Prien E. L., Gershoff S. N. Magnesium oxide-pyridoxine therapy for recurrent calcium oxalate calculi. J. Urol. (Baltimore) 1974; 112: 509.
  13. Fan J., Glass M. A., Chandhoke P. S. Impact of ammonium chloride administration on a rat ethylene glycol urolithiasis model. Scann. Microscopy 1999; 13: 299-306.
  14. Сивориновский Г. А. Количественное определение щавелевой кислоты в моче. Лаб. дело1969; 7: 401-404.
  15. Меньшиков В. В., Делекторская Л. Н., Золотнинская Р. М. Лабораторные методы исследования в клинике: Справочник. М.: Медицина; 1987. 266-267.
  16. Allegra A., Corica F. Plasma (total and ionized), erythrocyte and platelet magnesium levels in renal transplant recipients during cyclosporine and/or azathioprine treatment. Magnes. Res. 1998; 11 (1): 11-18.
  17. Garsia-Rodrigeuez M. B., Perez-Garsia C. C., Rios-Granja M. A. et al. Renal handling of calcium and phosphorus in experimental renal hyperparathyroidism in dogs. Vet. Res. 2003; 34: 379-387.
  18. Рыбина И. Л., Вощула В. И. Оценка роли метаболизма оксалата в этиопатогенезе мочекаменной болезни. БМЖ. 2006; 1: 15.
  19. Гланц С. Медико-биологическая статистика: Пер. с англ. М.: Практика; 1998.
  20. Dobson D. M., Finalayson B. Oxalate transport from plasma to interstinal lumen in the rat. Surg. Forum 1973; 24: 540.
  21. Sarica K., Erbagci A., Yagci F. et al. Limitation of apoptotic changes in renal tubular cell injury induced by hyperoxaluria. Urol. Res. 2004; 32 (4): 271-277.
  22. Firoz M., Graber M. Bioavailability of US commercial magnesium preparations. Magnes. Res. 2001; 14 (4): 257-262.
  23. Durlach J. Données actuelles sur les mécanismes de synergie entre vitamine B6 et magnésium. J. Méd. Besancon 1969; 5: 349- 359.
  24. Durlach J. Magnesium in clinical practice. London: John Libbey; 1988.
  25. Durlach J., Durlach V., Bac P. et al. Magnesium and therapeutics. Magnes. Res. 1994; 7 (3-4): 313-328.
  26. Curhan G. C., Willett W. C., Speizer F. E., Stampfer M. J. Intake of vitamins B6 and C and the risk of kidney stones in women. J. Am. Soc. Nephrol. 1999; 10 (4): 840-845.
  27. Wang P., Zhou M., Rana M. W. et al. ATP-MgCl2 restores renal microcirculation following trauma and severe hemorrhage. Can. J. Physiol. Pharmacol. 1992; 70 (3): 349-357.
  28. Rude R., Manoogian C., Ehrlich L. et al. Mechanisms of blood pressure regulation by magnesium in man. Magnesium 1989; 8 (5-6): 266-273.
  29. МакДермотт М. Т. Секреты эндокринологии: Пер. с англ. М.: Бином; 2001.

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