Experimental Models of Stress Urinary Incontinence


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Abstract

Despite numerous medical and surgical treatment strategies available, the problem of stress urinary incontinence (SUI) in women is still not completely resolved. Continuing research is underway to modify the sling operations and develop new bulk-enhancing agents, including the use of tissue engineering and cell technologies.
To evaluate the safety and effectiveness of new methods at the preclinical stage, adequate and reproducible experimental models of SUI in laboratory animals should be used.
This article presents analysis of all SUI models described in the scientific literature and the results of an experimental study comparing two primary ways of modeling, based on bilateral pudendal nerve damage in female rats.
The experiment results showed that only bilateral electrocoagulation of proximal part of pudendal nerves by the posterior approach ensured a stable and long-term SUI symptoms in animals in the form of leak point pressure reduction in the urodynamic study and increase of the of the urethral lumen according to histomorphometric analysis.
The results suggest that an adequate experimental SUI model is urethral rabdomiosphincter denervation by pudendal nerve electrocoagulation by the posterior surgical approach, when the nerve is damaged in the area of its separation from sciatic nerve. In this case stable and reproducible results are obtainable.

About the authors

A V Makarov

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

T H Fathudinov

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

E A Tuhovskaja

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry

I A Apolihina

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

I V Arutjunjan

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

A M Ismailova

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry

A V Elchaninov

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

E Ju Kananyhina

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

T A Teterina

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

G B Bolshakova

Research Institute of Human Morphology

Research Institute of Human Morphology

A V Vasilev

Research Institute of Human Morphology

Research Institute of Human Morphology

V V Glinkina

Russian National Research Medical University n.a. N.I. Pirogov

Russian National Research Medical University n.a. N.I. Pirogov

A N Murashev

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry

G T Suhih

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

V.I. Kulakov Research Center for Obstetrics, Gynecology andPerinatology

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