THE STUDY OF EFFECTIVENESS OF POLYHYDROXYALKANOATES BASED BIODEGRADABLE STENTS IN URETEROPELVIC SEGMENT PYELOPLASTY


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Abstract

Introduction Among urologic diseases, ureteropelvic segment stenosis with hydronephrosis is a common indication for instrumental or surgical correction. The restriction of urine flow with dilatation proximal to obstruction develops in 6.5-37% of cases at different times after the ureteral reconstruction. All this urges to develop and improve stents and search for effective ways to place stents and control their function. Aim To investigate the effectiveness of polyhydroxyalkanoates based biodegradable stent compared with a commercial analogue in upper urinary tract drainage after ureteropelvic segment pyeloplasty. Materials and methods Morphological and functional changes in the stented ureter were investigated in 45 male rabbits of «Soviet chinchilla» breed weighing 4550-5200 g that underwent stenting of ureteropelvic segment (UPS). The study used polymeric stents based on poly-3-hydroxybutyrate, poly-4-gidroksibutirotom P (3GB/4GB) and a mixture of poly-3-hydroxybutyrate with polycaprolactone II (3GB)/PCL with the inclusion of PCL 75%; the control material was polyurethane stents. Morphologic evaluation was conducted on ureteral fragments and UPSs in the area of the stent placement at 7, 14 and 28 days after operation. Results Throughout the experiment, excretory urography and spiral tomography in experimental groups showed no changes in the pelvicalyceal system after placing polymeric stents. The morphologic examination in the experimental group at day 28 after surgery revealed preserved longitudinal folding of ureteral mucosa and absence of muscle hypertrophy. Transitional epithelium had no signs of atrophy and desquamation, its mean thickness was 112.4±8.5 mm, whereas in the control group a productive inflammation resulting in sclerosis was found to develop. Conclusions We conducted a comparative study of morphologic and functional changes in rabbit ureters after stenting using polyurethane stents (control group) and polymeric stents made of poly-3-hydroxybutyrate, poly-4-hydroxybutyrate II (3GB /4GB) and a mixture of poly-3-hydroxybutyrate with polycaprolactone II (3Gb)/PCL (experimental group). Despite the difference in physical and mechanical properties of biodegradable PGA-based stents, the tissue response to both types of stent was comparable. Ureteral wall preserved longitudinal folds, there was no muscle layer hypertrophy, and mucous membrane had smooth contours with a uniform thickness of the transitional epithelium, whereas in the control group a productive inflammation resulting in sclerosis was found to develop.

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About the authors

M. P Myltygashev

Voino-Yasenetsky Krasnoyarsk State Medical University

Email: smallmirg@mail.ru
Teaching Assistant at the Department of Urology, Andrology and Sexology, IPE Krasnoyarsk, Russia

A. N Boyandin

Siberian Federal University

Email: araneus@mail.ru
PhD Biol.Sci, Laboratory of Biotechnology of New Biomaterials Krasnoyarsk, Russia

A. A Shumilova

Siberian Federal University

Email: shumilova.ann@mail.ru
PhD Biol.Sci., J.R. atthe Laboratory of Biotechnology of New Biomaterials Krasnoyarsk, Russia

F. P Kapsargin

Voino-Yasenetsky Krasnoyarsk State Medical University

Email: kapsargin@mail.ru
Dr.Med.Sci., Prof., Head of the Department of Urology, Andrology and Sexology, IPE Krasnoyarsk, Russia

E. I Shishatskaya

Siberian Federal University

Email: bio.sfu-kras.ru
Dr.Biol.Sci., Head of the Department of Medical Biology Krasnoyarsk, Russia

A. K Kirichenko

Voino-Yasenetsky Krasnoyarsk State Medical University

Email: krasak.07@mail.ru
Dr.Med.Sci. , Prof., Head of P.G. Podzolkov Department of Pathological Anatomy with the Course of PE Krasnoyarsk, Russia

T. G Volova

Institute of Biophysics, SB of the RAS

Email: volova4@mail.ru
Dr.Biol.Sci., Prof., Head of the Laboratory Chemoautotrophic Biosynthesis Krasnoyarsk, Russia

References

  1. Lum B. Intravesical chemotherapy of superficial bladder cancer, in Torti F (Ed): Urologic Cancer: Chemotherapeutic Principles and Management. Berlin, Springer-Verlag. 1983;3-36.
  2. Mishina T., Watanabe H., Kobayashi T., Maegawa M., Nakao M., Nakagawa S. Absorption of anticancer drugs through bladder epithelium. Urology. 1986;27:148-157.
  3. Au J.L., Badalament R.A., Wientjes M.G., Young D.C., Warner J.A., Venema P.L., Pollifrone D.L., Harbrecht J.D., Chin J.L., Lerner S.P., Miles B.J. Methods to improve efficacy of intravesical mitomycin C: results of a randomized phase III trial. J Natl Cancer Inst.2001;93:597-604.
  4. Chen D., Song D., Wientjes M.G., Au J.L. Effect ofdimethyl sulfoxide on bladder tissue penetration of intravesical paclitaxel. Clin Cancer Res.2003;9:363-369.
  5. Bartoletti R., Cai T., Gacci M., Giubilei G., Viggiani F., Santelli G., Repetti F., Nerozzi S., Ghezzi P., Sisani M. Intravesical gemcitabine therapy for superficial transitional cell carcinoma: results of a phase II prospective multicenter study. Urology.2005;66:726-731.
  6. Venkatraman S.S., Tan L.P., Joso J.F., Boey Y.C., Wang X. Biodegradable stents with elastic memory. Biomaterials. 2006;27:1573-1578.
  7. Brauers A., Thissen H., Pfannschmidt O., Bienert H., Foerster A., Klee D., Michaeli W., Höcker H., Jakse G. Development of a biodegradable ureteric stent: surface modification and in vitro assessment. J Endourol. 1997;11:399-403.
  8. Auge B.K., Ferraro R.F., Madenjian A.R., Preminger G.M. Evaluation of a dissolvable ureteral drainage stent in a swine model. J Urol. 2002;168:808-812.
  9. Dineen M.K., Tierney D.S., Kuzma P., Pentikis H.S. An evaluation of the pharmacokinetics and pharmacodynamics of the histrelin implant for the palliative treatment of prostate cancer. J Clin Pharmacol. 2005;45:1245-1249.
  10. Ramakumar S., Phull H., Purves T., Funk J., Copeland D., Ulreich J.B., Lai L.W., Lien Y.H. Novel deliveryof oligonucleotides using a topical hydrogel tissue sealant in a murine partial nephrectomy model. J Urol. 2005;174:1133-1136.
  11. Peppas N. Hydrogels and drug delivery. Curr Opin Colloid Interf Sci. 1997;2:531-537.
  12. Tyagi P., Chancellor M.B., Li Z., De Groat W.C., Yoshimura N., Fraser M.O., Huang L. Urodynamic and immunohistochemical evaluation of intravesical capsaicin delivery using thermosensitive hydrogel and liposomes. J Urol. 2004;171:483-489.
  13. Tyagi P., Li Z., Chancellor M., De Groat W.C., Yoshimura N., Huang L. Sustained intravesical drug delivery using thermosensitive hydrogel. Pharm Res. 2004;21:832-837.
  14. Morishita M., Lowman A.M., Takayama K., Nagai T., Peppas N.A. Elucidation of the mechanism of incorporation of insulin in controlled release systems based on complexation polymers. J Control Release. 2002;81:25-32.
  15. Peppas N., Langer R. Origins and development of biomedical engineering within chemical engineering. Aiche J. 2004;50:536-546.
  16. Волова Т.Г. Материалы для медицины, клеточной и тканевой инженерии [Электронный ресурс]: электрон. учеб. пособие / Т.Г. Волова, Е.И. Шишацкая, П.В. Миронов. Электрон. дан. (6 Мб). Красноярск, 2009
  17. Волова Т.Г., Шишацкая Е.И., Шишацкий О.Н. Биосовместимые полимеры. Наука в России. 2010;1:4-8
  18. Волова Т.Г., Севастьянов В.И., Шишацкая Е.И. Полигидроксиалканоаты (ПГА) - биоразрушаемые полимеры для медицин. Новосибирск: СО РАН. 2003;260 c
  19. Волова Т.Г., Шишацкая Е.И., Миронов П.В., Горева А.В. Структура и физико-химические свойства гибридного композита полигидроксибутират/волластонит. Перспективные материалы. 2009;1:43-50
  20. Волова, Т.Г. Разрушаемые биополимеры: получение, свойства, применение / Т.Г. Волова, Е.И. Шишацкая. - Красноярск: Изд-во «Красноярский писатель», 2011. 392 с
  21. Шишацкая Е.И., Волова Т.Г. Полигидроксиалканоаты как матриксы в клеточных технологиях. Клеточная трансплантология и тканевая инженерия. 2010;3:55-56
  22. Шишацкая Е.И., Камендов И.В., Старосветский С.И. Исследование остеопластических свойств матриксов из резорбируемого полиэфира гидроксимасляной кислоты. Клеточная трансплантология и тканевая инженерия. 2008;3(4):41-47
  23. Шишацкая Е.И., Винник Ю.С., Маркелова Н.М. Исследование остеопластических свойств резорбируемого поли-3-гидроксибутирата in vivo на моделях хронического остеомиелита. Врач аспирант. 2013;1.1.(56):127-132
  24. Шишацкая Е.И., Николаева Е.Д., Шумилова А.А., Шабанов А.В., Волова Т.Г. Культивирование и дифференцировка мультипотентных мезенхимальных клеток костного мозга на носителях из резорбируемого биопластотана. Клеточная трансплантология и тканевая инженерия. 2013;8(1):57-65

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