Structure of microorganism species cultured from urine of urolithiasis patients


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Abstract

Aim. To investigate the incidence of microorganisms of different taxonomic groups and their associations in the pelvic and bladder urine of adult urolithiasis patients. Materials and methods. A bacteriological method and metagenomic sequencing were used to investigate the bacterial spectrum of microflora cultured from pelvic and bladder urine sampled during surgical interventions in urolithiasis patients. Results. The both microbiotas had approximately the same spectrum, but in 26.1% of patients it was inconsistent. Metagenomic analysis detected DNA of microorganisms in urine samples which were found free of microflora by the bacteriological method. Conclusion. The study findings showed species diversity of microorganisms cultured from pelvic and bladder urine sampled during surgical interventions in urolithiasis patients.

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About the authors

M. D Kuz’min

Institute of Cellular and Intracellular Symbiosis, Ural Branch of the RAS; Orenburg Regional Clinical Hospital

Email: mdk-49@mail.ru
Dr.Med.Sci., the Honored Physician of the Russian Federation and the Republic of Mordovia, Urologist of the Highest Qualifying Category, Leading Researcher at the ICIS

Yu. I Peshkova

Institute of Cellular and Intracellular Symbiosis, Ural Branch of the RAS

Email: peshkovaji@mail.ru
Researcher at the Institute of Cellular and Intracellular Symbiosis

T. M Pashkova

Institute of Cellular and Intracellular Symbiosis, Ural Branch of the RAS

Email: pashkova070782@mail.ru
Ph.D. (biol.sci.), Senior Researcher at the Institute of Cellular and Intracellular Symbiosis

O. L Kartashova

Institute of Cellular and Intracellular Symbiosis, Ural Branch of the RAS

Email: labpersist@mail.ru
Dr.Biol.Sci., Head of the Laboratory of the Institute of Cellular and Intracellular Symbiosis

O. A Pashinina

Institute of Cellular and Intracellular Symbiosis, Ural Branch of the RAS

Email: olga25mikro@mail.ru
Ph.D. (biol.sci.), Senior Researcher at the Institute of Cellular and Intracellular Symbiosis

A. O Meshcheryakov

Orenburg State Medical University of Minzdrav of Russia

Email: amescheryakov1995@gmail.com
3rd Year Student of the Faculty of Medicine

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