THE INFLUENCE OF PROTEOLYTIC ENZYMES ON THE ANTIOXIDANT DEFENSIVE SYSTEM IN INFERTILE MARRIED MEN


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Abstract

The causes of male infertility remain unclear in 30-75% cases and are considered idiopathic. The study of influence of free oxygen radicals on the male reproductive function is of outmost interest. Given the important role of hyaluronidase in the penetration of sperm into the egg, we decided to evaluate the drug Longidaza, which has long-acting enzymatic (proteolytic) activity. A total of 48 men with idiopathic infertility were included in the study. All patients were divided into two groups (24 men each). In Group 1 there was a recommendation to attempt to conceive for 1 year without additional treatment. In Group 2, patients were prescribed Longidaza according to the proposed scheme. During the follow-up, there was a significant improvement in the antioxidant defense system of the ejaculate in Group 2, which was accompanied by an increase in the fertilizing ability of the ejaculate and an increase in number of conceptions by 29.1%

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About the authors

A. I Neumark

FGBOU VO «Altai State Medical University» of the Ministry of Health of Russia

Email: urologagmu@mail.ru
MD, professor, Head of the Department of Urology and Andrology with a course of Specialized Surgery Barnaul, Russia

B. A Neymark

FGBOU VO «Altai State Medical University» of the Ministry of Health of Russia

Email: urologagmu@mail.ru
MD, professor, professor at the Department of Urology and Andrology with a course of Specialized Surgery Barnaul, Russia

N. A Nozdrachev

FGBOU VO «Altai State Medical University» of the Ministry of Health of Russia

Email: nozdrachevuro@mail.ru
Ph.D., assistant professor at the Department of Urology and Andrology with course of specialized surgery Barnaul, Russia

V. M Bryukhanov

FGBOU VO «Altai State Medical University» of the Ministry of Health of Russia

MD, professor, professor at the Department of Pharmacology Barnaul, Russia

A. Yu Plotnikova

FGBOU VO «Altai State Medical University» of the Ministry of Health of Russia

Email: urologagmu@mail.ru
member of Student Scientific Society of the Department of Urology and Andrology with a course of Specialized Surgery Barnaul, Russia

D. A Podpruzhnikova

FGBOU VO «Altai State Medical University» of the Ministry of Health of Russia

Email: urologagmu@mail.ru
member of Student Scientific Society of the Department of Urology and Andrology with a course of Specialized Surgery Barnaul, Russia

A. A Nikulina

FGBOU VO «Altai State Medical University» of the Ministry of Health of Russia

Email: urologagmu@mail.ru
member of Student Scientific Society of the Department of Urology and Andrology with a course of Specialized Surgery Barnaul, Russia

References

  1. Божедомов В.А., Теодорович О.В. Эпидемиология и причины аутоиммунного мужского бесплодия. Урология. 2005;1:35-44.
  2. World Health Organization. Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucous Interaction. (1999) 4th edn. NewYork: Cambridge University Press.
  3. Тер-Аванесов Г.В. Андрологические аспекты бесплодного брака. Практическое руководство. М., 2000. С. 68.
  4. WHO manual for the standardized investigation and diagnosis of the infertile couple. WHO, 3-th ed.: Cambridge universiti press, 2000.
  5. Нишлаг Э., Бере Г.М. Андрология. Мужское здоровье и дисфункция репродуктивной системы: Перевод с английского. Медицинское информационное агентство, 2005.
  6. Tremellen K., Miari G., Froiland D., Thompson J. A randomised control trial examining the effect of an antioxidant (Menevit) on pregnancy outcome during IVF-ICSI treatment. Aust N Z J ObstetGynaecol. 2007;47:216-221.
  7. Гамидов С.И., Иремашвили В.В., Тхагопсоева П.А. Терапия нарушения фертильности у мужчин: перспективные результаты европейских исследований. Эффективная фармакотерапия в урологии. 2009;2:26-30.
  8. Зоркин С.Н., Чеботаев И.Л., Смирнов И.Е., Баканов М.И. Оксид азота, как показатель эндотелиальной дисфункции при варикоцеле. Материалы Международного Конгресса по андрологии 28-29 мая 2009, Сочи OK «Дагомыс» УД Президента РФ. 2009. С. 144-145.
  9. Agarwal A., Said T.M. Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach. BJU Int. 2008;95:503-507.
  10. De Lamirande E., Gagnon C. The dark and bright sides of reactive oxygen species on sperm function. In: Gagnon C. (ed) The male gamete: from basic science to clinical application. Cache River Press, Vienna, 1999. P. 455-467.
  11. Aitken R.J., Gordon E., Harkiss D., Twigg J.P., Milne P., Jennings Z., Irvine D.S. Relative impact of oxidative stress on the functional competence andgenomic integrity of human spermatozoa. Biol Reprod. 1998;59:1037-1046.
  12. Gromenko D.S. The use of high technologies for an assessment of male fertility. Vestnik medicinskyh technologyi. 2008;XV(4):118-120. Russian (Громенко Д.С. Применение наукоемких технологий для оценки фертильности мужчин. Вестник новых медицинских технологий. 2008;XV(4):118-120).
  13. Bonduelle M. et al. Mental development of 201 ICSI children at 2 years of age. Lancet. 1998, 351.
  14. Bowen J.R. et al. Medical and developmental outcome at 1 year for children conceived by intracytoplasmic sperm injection. Lancet. 1998;351:1529- 1534.
  15. Zorn B., Sesek-Briski A., Osredkar J., Meden-Vrtovec H. Semen polymorphonuclear neutrophil leukocyte elastase as a diagnostic and prognostic marker of genital tract inflammation-a review. Clin Chem Lab Med. 2003;41:2-12.
  16. Aitken R.J., Harkiss, Knox W. et al. A novel signal transduction cascade in capacitating human spermatozoa characterised by a redox-regulated, cAMPmediated induction of tyrosine phosphorylation. Cell. Sci. 1998;Ill (Pt 5):645-656.
  17. Henkel R., Hanschke A., Miska W. et al. Impairment of sperm motilty by inflammatory parameters. Reprod. Domest. Anim. 1999;34(1):26.
  18. Kao S.H., Chao H.T., Chen H.W., Hwang T.I., Liao T.L., Wei Y.H Increase of oxidative stress in human sperm with lower motility. Fertil Steril. 2007. 30 July, 2007, online publication ahead of print.
  19. Kemal Duru N., Morshedi M., Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil Steril. 2000;74(6):1200-1207.
  20. Loft S., Kold-Jensen T., Hjollund N.H., Giwercman A., Gyllemborg J., Ernst E., Olsen J., Scheike T., Poulsen H.E., Bonde J.P. Oxidative DNA damage in human sperm influences time to pregnancy. Hum Reprod. 2003;18:1265-1272.
  21. Meseguer M., de los Santos M.J., Simon C., Pellicer A., Remohi J., Garrido N. Effect of sperm glutathione peroxidases 1 and 4 on embryo asymmetry and blastocyst quality in oocyte donation cycles. Fertil Steril. 2006;86:1376-1385.
  22. Tremellen K., Miari G., Froiland D., Thompson J. A randomised control trial examining the effect of an antioxidant (Menevit) on pregnancy outcome during IVF-ICSI treatment. Aust N Z J Obstet Gynaecol. 2007;47:216-221.
  23. Реброва О.Ю. Статистический анализ медицинских данных. Применение пакета прикладных программ STATISTICA. М.: Изд-во Медиа Сфера, 2006. 305 с..

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