POLYAMINES OF UROGENITAL TRACT MEN AS FACTORS OF APOPTOSIS REGULATION SPERMATOZOIDS


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Abstract

The aim of the study was to compare the content of polyamines (spermine and spermidine) in the seminal plasma of men of different fertility and to reveal the relationship between their level in sperm and the presence of markers of apoptosis in gametes. Materials and methods: ejaculates of 34 fertile men and 40 infertile men with various forms of subfertility between the ages of 22 and 40 were examined. The determination of polyamines was carried out by electrophoretic fractionation in an agar gel. As markers of apoptosis, externalization of phosphatidylserine (PS) to the external side of the spermatozoon membrane was determined and the receptor for the initiation of apoptosis CD95 in the gametes was detected. Results: In sperm of infertile men, more spermatozoa with markers of apoptosis were revealed in comparison with fertile donors (p 0,001). Against the background of a general decrease in the concentration of polyamines in ejaculates of infertile patients, the ratio between polyamines with a predominance of sperm in the seminal fluid is observed, which is the initiating factor for the initiation of apoptosis in gametes. This was proved by the relationship between the externalization of FS in gametes and the concentration of sperm in the spermoplasm in accordance with the coefficient of positive correlation (r=0.5, p<0.01). Conclusions: Thus, a change in the concentration of polyamines in the seminal fluid of men is one of the factors in the regulation of apoptosis of the sex cells. Determination of the content of polyamines in seminal plasma can be recommended to increase the information content of the study of the causes of impaired fertility of the ejaculate, and the results obtained for the development of the algorithm for examining infertile patients.

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About the authors

M. V Ploskonos

Astrakhan State Medical University Health Ministry of Russian Federation

Email: ploskonoz@mail.ru
doctor of biological sciences, assistant professor, Astrakhan State Medical University Health Ministry of Russian Federation Astrakhan, Russia

V. V Evdokimov

National Medical Research Radiologmal Center of the Ministry of Health of Russian Federation

MD, chief researcher N. Lopatkin Scientific Research Institute of Urology and Interventional Radiology - branch Moscow, Russia

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