A novel method for non-invasive mechanical ablation of prostate tumors using pulsed focused ultrasound


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Abstract

Aim ofthe study: demonstrate the feasibility of non-invasive mechanical disintegration of human prostate tissue using pulsed high-intensity focused ultrasound (pHIFU), a method termed boiling histotripsy. Materials and methods. An ultrasound experimental system was developed for producing localized mechanical lesions in ex vivo biological tissue samples under ultrasound guidance. A series of experiments was carried out to create small single-focus lesions (volume < 2 mm3) and one large lesion (volume > 50 mm3) in ex vivo prostate tissue samples. After irradiation, two samples were bisected to visualize the region of destruction; the other tissue samples were examined histologically. Results. During pHIFU irradiation under B-mode ultrasound guidance, a region of increased echogenicity caused by formation of vapor-gas bubbles was visualized in the target region. After exposure, small and large lesions filled with a suspension of liquefied tissue were observed. Histological examination confirmed that the prostate tissue in the focal region was disintegrated into subcellular fragments. Conclusion. A pilot study showed the feasibility of using boiling histotripsy as a non-invasive method for treating prostate diseases.

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About the authors

V. A Khokhlova

Lomonosov Moscow State University

Email: vera@acs366.phys .msu.ru
DSc in Physics, Associate professor at the Department of Acoustics, Physics Faculty Moscow, Russia

P. B Rosnitskiy

Lomonosov Moscow State University

Email: pavrosni@yandex.ru
Junior Research scientist at the Department of Medical Physics, Physics Faculty Moscow, Russia

S. A Tsysar

Lomonosov Moscow State University

Email: sergey@acs366.phys.msu.ru
PhD in Physics, Associate professor at the Department of Photonics and Physics of Microwaves, Physics Faculty Moscow, Russia

S. V Buravkov

Lomonosov Moscow State University

Email: buravkov@fbm.msu.ru
MD, Leading Research scientist at the Laboratory of Image Analysis of Cell Structures, Faculty of Fundamental Medicine Moscow, Russia

O. A. Sapozhnikov

Lomonosov Moscow State University

Email: oleg@acs366.phys.msu.ru
DSc in Physics, Professor at the Department of Acoustics, Physics Faculty Moscow, Russia

M. M Karzova

Lomonosov Moscow State University

Email: karzova@physics .msu.ru
PhD in Physics, Junior Research scientist at the Department of Acoustics, Physics Faculty Moscow, Russia

T. D Khokhlova

University of Washington

Email: tdk7@uw.edu
PhD in Physics, Assistant professor at the Department of Medicine Seattle, USA

A. D Maxwell

University of Washington

Email: amax38@uw.edu
PhD in Biomedical Engineering, Assistant professor at the Department of Urology Seattle, USA

N. M Gaifullin

Lomonosov Moscow State University

Email: gaifulin@rambler.ru
MD, Head of the Department of Pathology and Anatomy, Medical Research and Educational Center, Deputy Dean of the Faculty of Fundamental Medicine Moscow, Russia

A. V Kadrev

Lomonosov Moscow State University

Email: akadrev@yandex.ru
MD, Head of the Department of Ultrasound Diagnostics, Medical Research and Educational Center Moscow, Russia

D. A Okhobotov

Lomonosov Moscow State University

Email: 14072003m@mail.ru
MD, Senior Research scientist at the Department of Urology and Andrology, Medical Research and Educational Center, Vice Chair on Education at the Department of Urology and Andrology, Faculty of Fundamental Medicine Moscow, Russia

A. A Kamalov

Lomonosov Moscow State University

Email: armais.kamalov@rambler.ru
Academician of the RAS, MD, Professor, Director of Medical Research and Educational Center, Head of the Department of Urology and Andrology, Faculty of Fundamental Medicine Moscow, Russia

G. R Schade

University of Washington

Email: grschade@uw.edu
MD, Assistant professor at the Department of Urology Seattle, USA

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