The efficiency of pre-administration of a peptide mimicking the spatial structure of erythropoietin α-chain B in modeling experimental post-contrast acute kidney injury
- Authors: Bratchikov O.I.1, Kondrashov М.М.1, Kostina D.A.2, Shcheblykina O.V.2
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Affiliations:
- Kursk State Medical University of the Ministry of Health of the Russian Federation
- Belgorod National Research University
- Issue: No 6 (2023)
- Pages: 64-67
- Section: Original Articles
- URL: https://journals.eco-vector.com/1728-2985/article/view/626069
- DOI: https://doi.org/10.18565/urology.2023.6.64-67
- ID: 626069
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Abstract
Aim. To study the efficiency of pre-administration of a peptide mimicking the spatial structure of erythropoietin α-chain B in modeling experimental post-contrast acute kidney injury.
Materials and methods. In this study, an experimental model of post-contrast acute kidney injury was created using a non-steroidal anti-inflammatory drug, a nitric oxide synthase inhibitor, and injection of iopromide to mature male mice. After 48 hours, a comprehensive assessment of the concentration of creatinine, urea, glomerular filtration rate, the ratio of urea/albumin in the serum, as well as the level of proteinuria and interleukin 6 in the urine was carried out.
Results. A peptide mimicking the spatial structure of erythropoietin α-chain B, administered at a dose of 100 μg/kg 30 minutes before modeling of pathologic process, contributes to a significant decrease in creatinine and urea concentrations by 2.5 and 1.8 times, respectively, with an increase in glomerular filtration rate 4.4 times. In addition, in the group with pharmacological correction, there was a significant decrease in the ratio of urea/albumin by 2.2 times, a decrease in the level of proteinuria by 61.9% and a decrease in the concentration of pro-inflammatory interleukin-6 in the urine by 2.1 times.
Conclusion. Thus, the preliminary administration of a peptide that mimics the spatial structure of the erythropoietin α-chain B helps to reduce the severity of post-contrast acute kidney injury in the experiment, due to anti-inflammatory properties.
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About the authors
O. I. Bratchikov
Kursk State Medical University of the Ministry of Health of the Russian Federation
Author for correspondence.
Email: BratchikovOI@kursksmu.net
Ph.D., MD, Head of the Department of Urology
Russian Federation, KurskМ. М. Kondrashov
Kursk State Medical University of the Ministry of Health of the Russian Federation
Email: Kondra.0317@mail.ru
ORCID iD: 0009-0007-1479-3557
Assistant of the Department of Urology
Russian Federation, KurskD. A. Kostina
Belgorod National Research University
Email: daria-f13@mail.ru
ORCID iD: 0000-0002-4505-3988
Ph.D., Associate Professor of the Department of Pharmacology and Clinical Pharmacology
Russian Federation, BelgorodO. V. Shcheblykina
Belgorod National Research University
Email: sheolvi31@gmail.com
ORCID iD: 0000-0003-0346-9835
Ph.D., Associate Professor of the Department of Pharmacology and Clinical Pharmacology
Russian Federation, BelgorodReferences
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