Pathomorphological changes in ejaculate revealed by electron transmission microscopy in patients who have had COVID-19

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Introduction: Disturbances due to the novel coronavirus infection (COVID-19) caused economic, physical and psychological damage to the population of the whole world. The number of people who have suffered SOVID-19 is growing, and there is a number of negative consequences are documented for many organs and systems of the body, including reproductive.

Aim. To study the pathomorphological changes in ejaculate revealed by transmission electron microscopy in patients who have had COVID-19.

Materials and methods. The results of the electronic transmission microscopy of ejaculate 157 men were analyzed. They were divided into four groups. In group 1, there were 49 patients with idiopathic infertility who suffered COVID-19, group II included 42 patients with idiopathic infertility without prior of history of COVID-19, group III consisted of 39 fertile men who have had COVID-19, while in group IV there were 27 fertile men without prior of history of COVID -19.

Results. In most cases, pathological changes detected during morphological examination of patients before and after inclusion in the study were detected in the head of the sperm, which were resulted in a small specific gravity, but 12 months after infection with SARS-CoV-2, the percentage of acrosome changes increased in groups I and III. Moreover, combined pathological changes in the acrosome were recorded.

Conclusions. Thus, transmission electron microscopy revealed morphometric sperm disorders that were associated with energy processes in the cell, which naturally should be taken into account when rehabilitation of reproductive men who have had SARS-COV-2.

In addition, in patients of SARS-COV-2, a severe cell pathology was noted, due to the residualization of the cytoplasm and the pathology of mitochondria.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Khalid Ibishev

FGBOU VO Rostov State Medical University of the Ministry of Health of the Russian Federation

Хат алмасуға жауапты Автор.
Email: ibishev22@mail.ru

Ph.D., MD, Professor of the Department of Urology and Human Reproductive Health (with a course of pediatric urology and andrology)

Ресей, 344022, Rostov-on-Don, lane Nakhichevan, 29, buil. 9

Yan Prokop

FGBOU VO Rostov State Medical University of the Ministry of Health of the Russian Federation

Email: unprocop@mail.ru
ORCID iD: 0000-0001-5340-9406

Ph.D. student at the Department of Urology and Human Reproductive Health (with a course of pediatric urology and andrology)

Ресей, 344022, Rostov-on-Don, lane Nakhichevan, 29, buil. 9

Sergey Todorov

FGBOU VO Rostov State Medical University of the Ministry of Health of the Russian Federation

Email: sertodorov@gmail.com
ORCID iD: 0000-0001-8476-5606

Ph.D., MD, Head of the Department of Pathological Anatomy

Ресей, 344022, Rostov-on-Don, lane Nakhichevan, 29, buil. 9

Elena Sinelnik

FGBOU VO Rostov State Medical University of the Ministry of Health of the Russian Federation

Email: 127pao.ob2@mail.ru
ORCID iD: 0000-0002-2201-5065

a doctor of the highest category, assistant of the Department of Pathological Anatomy of Rostov State Medical University

Ресей, 344022, Rostov-on-Don, lane Nakhichevan, 29, buil. 9

Dmitry Sizyakin

FGBOU VO Rostov State Medical University of the Ministry of Health of the Russian Federation

Email: dsiziakin@mail.ru
ORCID iD: 0000-0002-9627-2582

Ph.D., MD, professor, professor of the Department of Urology and Human Reproductive Health (with a course of pediatric urology and andrology)

Ресей, 344022, Rostov-on-Don, lane Nakhichevan, 29, buil. 9

Mikhail Kogan

FGBOU VO Rostov State Medical University of the Ministry of Health of the Russian Federation

Email: dept_kogan@mail.ru
ORCID iD: 0000-0002-1710-0169

Ph.D., MD, professor, Honored Scientist of the Russian Federation, Head of the Department of Urology and Human Reproductive Health (with a course of pediatric urology and andrology)

Ресей, 344022, Rostov-on-Don, lane Nakhichevan, 29, buil. 9

Әдебиет тізімі

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Әрекет
1. JATS XML
2. Fig. 1. Ejaculate of patient group I. Group of spermatozoa with numerous head shape anomalies, reacted and abnormal acrosomes. Electron diffraction pattern, magnification × 10,000

Жүктеу (8MB)
3. Fig. 2. Group of spermatozoa with multiple head shape anomalies, reacted and abnormal acrosomes. Electron diffraction pattern, magnification ×10,000

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4. Fig. 3. Ejaculate of the patient of group I. The head is of an abnormal elongated shape, the acrosome is destroyed, the post-acrosomal plate is absent, “immature chromatin”, the chromatin is destroyed. Electron diffraction pattern, magnification ×25,000

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5. Fig. 4. Ejaculate of a patient of group I. The head has an abnormal “spherical” shape, “immature chromatin”, and a destroyed acrosome. Electron diffraction pattern, magnification ×25,000.

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6. Fig. 5. Ejaculate of the patient of group I. The head is of abnormal shape, residual cytoplasm. "Swollen" mitochondria of the neck without cristae. Electronogram, magnification × 30,000

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