Characteristics of the taxonomic structure of the microbiota of testicles and urethra in men with non-obstructive azoospermia with different outcomes in ART protocols

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Aim. To carry out a comparative analysis of the taxonomic structure of the testicular and urethral microbiota of patients with non-obstructive azoospermia (NOA) in ART protocols with positive (live birth) and negative outcomes.

Materials and methods. The samples of testicular tissue and urethra of infertile patients with NOA (n=62) were evaluated. To realize the reproductive potential, all patients underwent micro-TESE in the ART protocol. All patients were retrospectively divided into two groups. In group 1, there were patients with NOA and a positive ART result (n=16), while in group 2 patients had NOA and a negative ART result (n=46). To study the bacterial diversity of testicular tissue, an analysis of amplicons of the bacterial 16S rRNA gene was performed using high-throughput NGS.

Results. A comparative analysis of the relative representation of bacterial taxa in the testicular tissue of patients with NOA with positive and negative ART outcomes revealed a number of significant differences. For example, significant differences (p<0.05) were found in the relative representation of the phylum Fusobacteriota, the Pasteurellaceae, Dialisteraceae, Porphyromonadaceae, Alcanivoracaceae, Neisseriaceae_563222, Ruminococcaceae, Acutalibacteraceae, Peptostreptococcaceae_256921, Marinilabiliaceae, Exiguobacteraceae, Coprobacillaceae, Bacillaceae_H_289398, Burkholderiaceae_A_595427 in patients of group 1. Significant differences (p<0.05) were also found in the frequency of occurrence of the phylum Fusobacteriota, the families Lactobacillaceae, Pasteurellaceae, Alcanivoracaceae, Acutalibacteraceae, Peptostreptococcaceae_256921, Exiguobacteraceae, Coprobacillaceae, Bacillaceae_H_289398, Burkholderiaceae_A_595427 in patients with positive ART outcomes. When analyzing the urethral microbiome using high-throughput sequencing, no significant differences in alpha-diversity indices were shown. We also found significant differences (p<0.05) in the frequency of occurrence of the Streptococcaceae family, and they were more common in patients with negative ART outcomes. Meanwhile, representatives of the Enterococcaceae and Brevibacteriaceae families were more common in patients with positive ART outcomes.

Conclusions. It is obvious that changes in the microbiota of the genital tract have a specific effect on the reproductive system, and correction of abnormal microbiomes can improve reproductive outcomes.

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作者简介

Mikhail Faniev

Peoples’ Friendship University of Russia

编辑信件的主要联系方式.
Email: faniev.mv@gmail.ru
ORCID iD: 0000-0002-7323-3126

Ph.D., associate professor at the Department of Endoscopic Urology and Ultrasound Diagnostics, Faculty of Professional Development for Health Care Employees, Medical Institute

俄罗斯联邦, Moscow

Zeiratho Kadyrov

Peoples’ Friendship University of Russia

Email: zieratsho@yandex.ru
ORCID iD: 0000-0002-1108-8138

Ph.D., MD, professor, Head of the Department of Endoscopic Urology and Ultrasound Diagnostics, Faculty of Professional Development for Health Care Employees, Medical Institute

俄罗斯联邦, Moscow

Nadezhda Druzhinina

Peoples’ Friendship University of Russia

Email: kvdrdnk@mail.ru
ORCID iD: 0000-0003-3277-6068

Ph.D. student at the Department of Endoscopic Urology and Ultrasound Diagnostics, Faculty of Professional Development for Health Care Employees, Medical Institute

俄罗斯联邦, Moscow

Vladimir Stepanov

Peoples’ Friendship University of Russia

Email: stepanovvs1@yandex.ru
ORCID iD: 0000-0003-0525-3026

leading urologist at the medical holding SM Clinic

俄罗斯联邦, Moscow

Yaroslav Prokopev

FGBOU DPO “Russian Medical Academy of Continuing Professional Education” of the Ministry of Health of Russian Federation

Email: yar80@mail.ru
ORCID iD: 0000-0002-4345-127X

Ph.D., associate professor at the Department of Urology and Nephrology of Kazan State Medical Academy

俄罗斯联邦, Kazan

Tatyana Fedorenko

GBUZ Krasnodar Pediatric City Clinical Hospital

Email: fedorenko.tv@mail.ru
ORCID iD: 0009-0008-9780-4158

Ph.D. in Biology, Biologist of the Laboratory of cellular technologies of the Center for Reproductive and Cellular Medicine Diagnostic Center

俄罗斯联邦, Krasnodar

Maria Markelova

Kazan (Volga Region) Federal University

Email: mimarkelova@gmail.com
ORCID iD: 0000-0001-7445-2091

Ph.D. in Biology, senior researcher at the Research laboratory "Multi-omics technologies of living systems" of the Institute of Fundamental Medicine and Biology

俄罗斯联邦, Kazan

Dilyara Khusnutdinova

Kazan (Volga Region) Federal University

Email: dilyahusn@gmail.com
ORCID iD: 0000-0002-9982-9059

Ph.D. in Biology, senior researcher at the Research laboratory "Multi-omics technologies of living systems" of the Institute of Fundamental Medicine and Biology

俄罗斯联邦, Kazan

Tatyana Grigoryeva

Kazan (Volga Region) Federal University

Email: tatabio@inbox.ru
ORCID iD: 0000-0001-5314-7012

Ph.D. in Biology, senior researcher at the Research laboratory "Multi-omics technologies of living systems" of the Institute of Fundamental Medicine and Biology

俄罗斯联邦, Kazan

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2. Fig. 1. Significantly different bacterial taxa in the microbiota of testicular tissue in patients with NOA with positive and negative ART results

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3. Fig. 2. Significantly different bacterial families in the urethral microbiota of patients with NOA with positive and negative ART results

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