HYPERBARIC OXYGEN THERAPY IN THE TREATMENT OF MALE INFERTILITY ASSOCIATED WITH INCREASED SPERM DNA FRAGMENTATION AND REACTIVE OXYGEN SPECIES IN SEMEN


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Abstract

The aim of this study was to explore the potential of hyperbaric oxygenation (HBO) for reduction of sperm DNA fragmentation level and reactive oxygen species (ROS) in semen. The study included 90 men with idiopathic infertility. Patients of the treatment group (n=60) underwent HBO before the vitro fertilization (IVF) procedure. In the control group (n=30) IVF was carried out without prior course of HBO. Sperm DNA fragmentation analysis was carried out using the TUNEL assay, the level of ROS in the ejaculate was measured by chemiluminescence. HBO treatment resulted in a significant decrease in the mean level of sperm DNA fragmentation from 33.2±7.5 to 11.9±5.9%, and the median ROS in sperm from 0.89 to 0.39 mV/s (p<0,05). In the control group these changes were not statistically significant. Pregnancy after IVF occurred in 63.3% (38/60) of sexual partners of the treatment group men and in 36.7% (11/30) of the control group (p<0.05). The high efficiency of HBO in overcoming the adverse effects of oxidative stress on sperm parameters allows us to consider it as a promising method for the treatment of men with idiopathic infertility.

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About the authors

A. Yu Metelev

S.P Botkin City Clinical Hospital

Email: urometelev@mail.ru
M.D., Urologist

A. B Bogdanov

Russian Medical Academy of Postgraduate Education of Minzdrav of Russia; S.P Botkin City Clinical Hospital

Department of Urology and Surgical Andrology

E. V Ivkin

Russian Medical Academy of Postgraduate Education of Minzdrav of Russia; S.P Botkin City Clinical Hospital

Department of Urology and Surgical Andrology

A. A Mitrokhin

S.P Botkin City Clinical Hospital

M. M Vodneva

S.P Botkin City Clinical Hospital

E. I Veliev

Russian Medical Academy of Postgraduate Education of Minzdrav of Russia; S.P Botkin City Clinical Hospital

Department of Urology and Surgical Andrology

References

  1. Jungwirth A., Giwercman A., Tournaye H., Diemer T., Kopa Z., Dohle G., Krausz C.; European Association of Urology Working Group on Male Infertility. European Association of Urology guidelines on Male Infertility: the 2012 update. Eur. Urol. 2012;62(2):324-332.
  2. Богданов Ю.А., Карпунина Т.И., Зуева Т.В. К вопросу о распространенности мужского бесплодия. Медицина и образование в Сибири. 2013;5.
  3. Божедомов В.А., Рохликов И.М., Третьяков А.А., Липатова Н.А., Виноградов И.В., Никонов Е.Л. Актуальные вопросы оказания помощи парам с мужским фактором бездетного брака: клинические и организационно-методические аспекты. Андрология и генитальная хирургия. 2013;4:7-16.
  4. Тюзиков И.А., Калинченко С.Ю., Ворслов Л.О., Тишова Ю.А. Витамин D, мужское здоровье и мужская репродукция. Андрология и генитальная хирургия. 2013;4:36-44.
  5. Sabanegh E.J., Agarwal A. Male infertility. In: Campbell-Walsh urology. Campbell M.F., Walsh P.C., Wein A.J., eds. 10th ed. Philadelphia: Saunders Elsevier, 2012. P. 616-647.
  6. Khazaie Y., Nasr Esfahani M.H. MicroRNA and male infertility: a potential for diagnosis. Int. J. Fertil. Steril. 2014;8(2):113-118.
  7. Kamel R.M. Management of the infertile couple: an evidence-based protocol. Reprod. Biol. Endocrinol. 2010;8(21).
  8. Lewis S.E., John Aitken R., Conner S.J., Iuliis G.D., Evenson D.P., Henkel R., Giwercman A., Gharagozloo P. The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. Reprod. Biomed. Online. 2013;27(4):325-337.
  9. Wyrobek A.J., Eskenazi B., Young S., Arnheim N., Tiemann-Boege I., Jabs E.W., Glaser R.L., Pearson F.S., Evenson D. Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. Proc. Natl. Acad. Sci. USA. 2006;103(25):9601-9606.
  10. Benchaib M., Lornage J., Mazoyer C., Lejeune H., Salle B., François Guerin J. Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil. Steril. 2007;87(1):93-100.
  11. Walczak-Jedrzejowska R., Wolski J.K., Slowikowska-Hilczer J. The role of oxidative stress and antioxidants in male fertility. Cent. European. J. Urol. 2013;66(1):60-67.
  12. Chen S.J., Allam J.P., Duan Y.G., Haidl G. Influence of reactive oxygen species on human sperm functions and fertilizing capacity including therapeutical approaches. Arch. Gynecol. Obstet. 2013;288(1):191-199.
  13. World Health Organization. WHO laboratory manual for the examination and processing of human semen. Fifth edition. Geneva: WHO, 2010.
  14. Bungum M., Bungum L., Giwercman A. Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility. Asian. J. Androl. 2011;13(1):69-75.
  15. Evenson D.P. Sperm chromatin structure assay (SCSA®). Methods. Mol. Biol. 2013;927:147-164.
  16. Avendano C., Oehninger S. DNA fragmentation in morphologically normal spermatozoa: how much should we be concerned in the ICSI era? J. Androl. 2011;32(4):356-363.
  17. Giwercman A., Lindstedt L., Larsson M., Bungum M., Spano M., Levine R.J., Rylander L. Sperm chromatin structure assay as an independent predictor of fertility in vivo: a case-control study. Int. J. Androl. 2010;33(1):e221-227.

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