Metagenomic analysis of samples in cystitis
- 作者: Tets G.V1, Tets V.V1, Voroshilova T.M2
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隶属关系:
- First Pavlov State Medical University of St. Peterburg of Minzdrav of Russia
- 期: 编号 1 (2016)
- 页面: 29-31
- 栏目: Articles
- URL: https://journals.eco-vector.com/1728-2985/article/view/282346
- ID: 282346
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详细
Detecting bacteria in pathological material and testing their sensitivity to antibiotics is essential in determining a treatment strategy and the choice of antimicrobial therapy. The results of the study showed that metagenomic analysis detects more bacteria in the pathological material than standard laboratory methods. We found a variety of bacteria, described as causative agents of urinary tract infections, evading the standard culture conditions used in routine diagnostic laboratories. These data show that in urinary tract infections mixed pathogens may be much more common than usually believed. At that, the pathological process involves bacteria with unexplored pathogenicity and sensitivity to antibiotics.
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作者简介
G. Tets
First Pavlov State Medical University of St. Peterburg of Minzdrav of Russia
Email: georgetets@gmail.com
The Department of Microbiology and Virology
V. Tets
First Pavlov State Medical University of St. Peterburg of Minzdrav of Russia
Email: vtetzv@yahoo.com
The Department of Microbiology and Virology
T. Voroshilova
参考
- Grice E.A., Segre J.A. The Human Microbiome: Our Second Genome. Annu. Rev. Genomics Hum. Genet. 2012; 13: 151-170.
- Oliver J.D. Recent findings on the viable but nonculturable state in pathogenic bacteria. FEMS Microbiol. Rev. 2010; 34: 415-425.
- Lipkin W.I. Lipkin W.I. The changing face of pathogen discovery and surveillance. Nat. Rev. Microbiol. 2013; 11: 133-141.
- Petrosino J.F., Highlander S., Luna R.A., Gibbs R.A., Versalovic J. Metagenomic pyrosequencing and microbial identification. Clin. Chem. 2009;55(5):856-866.
- Schloss P.D., Westcott S.L., Ryabin T., Hall J.R., Hartmann M., Hollister E.B., Lesniewski R.A., Oakley B.B., Parks D.H., Robinson C.J., Sahl J.W., Stres B., Thallinger G.G., Van Horn D.J., Weber C.F. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl. Environ. Microbiol. 2009;75(23):7537-7541.
- Cruz A.T., Cazacu A.C., Allen C.H. Pantoea agglomerans, a plant pathogen causing human Disease. J. Clin. Microbiol. 2007; 45(6): 1989-1992.
- Mezzatesta M.L., Gona F., Stefani S. Enterobacter cloacae complex: clinical impact and emerging antibiotic resistance. Future Microbiol. 2012;7(7):887-902. doi: 10.2217/fmb.12.61.
- Fraser S.L., Sinave S. P., Mileno M.D. Enterobacter Infections: Practice Essentials, Background, Pathophysiology. Updated Mar 13, 2014 http://www.emedicine.medscape.com/article/216845-overview
- Фельдблюм И.В., Захарова Ю.А., Деменко С.Г. Сравнительная оценка различных подходов к изучению заболеваемости гнойно-септическими инфекциями среди родильниц в акушерских стационарах. Медицинский альманах. 2011;5:209-212
- O'Hara C.M., Steigerwalt A.G., Hill B.C., Farmer J.J. 3rd, Fanning G.R., Brenner D.J. Enterobacter hormaechei, a new species of the family Enterobacteriaceae formerly known as enteric group 75. J. Clin. Microbiol. 1989;27(9):2046-2049.
- Garazzino S., Aprato A., Maiello A., Masse A., Biasibetti A., De Rosa F.G., Di Perri G. Osteomyelitis caused by Enterobacter cancerogenus infection following a traumatic Injury: case report and review of the literature. J. Clin. Microbiol. 2005;43(3):1459-1461.
- Papasian S.J., Enna-Kifer S., Garrison B. Symptomatic Shigella sonnei urinary tract infection. J. Clin. Microbiol. 1995; 33(8): 2222-2223.
- John G.J. Jr., Sharbaugh R.G., Bannister E.R. Enterobacter cloacae: bacteremia, epidemiology, and antibiotic Resistance. Rev. Infect. Dis. 1982;4(1):13-28.
- DeLappe N., O'Halloran F., Fanning S., Corbett-Feeney G., Cheasty T., Cormican M. Antimicrobial resistance and genetic diversity of Shigella sonnei isolates from Western Ireland, an area of low incidence of infection. J. Clin. Microbiol. 2003;41:1919-1924.
- Тец В.В., Тец Г.В., Викина Д.С., Вечерковская М.Ф., Харламова В.В. Неизвестные возбудители заболеваний в микрофлоре ротовой полости человека, актуальные для оториноларингологии. Вестник оториноларингологии. 2014; 1: 33-36
- Вечерковская М.Ф., Тец Г.В., Тец В.В. Неизвестные бактерии в микрофлоре ротовой полости детей с онкогематологическими заболеваниями. Клиническая онкогематология. 2014;7(2): 229-232