The additional criterion for the diagnosis of purulent-destructive forms of acute pyelonephritis
- 作者: Shchuplova E.A1, Kuzmin M.D1,2
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隶属关系:
- Institute of Cellular and Intracellular Symbiosis, Ural Branch of the RAS
- Orenburg Regional Clinical Hospital
- 期: 编号 2 (2019)
- 页面: 5-8
- 栏目: Articles
- URL: https://journals.eco-vector.com/1728-2985/article/view/322045
- DOI: https://doi.org/10.18565/urology.2019.2.5-8
- ID: 322045
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Aim: to develop an additional criterion for objective diagnosis of purulent-destructive forms of acute pyelonephritis. Materials and methods. A total of 35 blood samples from patients with acute pyelonephritis aged from 19 to 85 years (mean age was 52 years) were studied. For the analysis, a classical bacteriological method of isolating blood culture and a modern molecular genetic method of fluorescent in situ hybridization (FISH) using DNA probes complementary to species-specific regions of the 16S rRNA gene of microorganisms were used. The obtained results were processed using the Statistica 6.0 program. Results. The bacteriological study showed that only in one patient (2.9%) a blood culture was positive, whereas with FISH, polymicrobial infection consisting of two associates was observed in five patients (14.3%), and 207 specific luminescence from different DNA probes were detected in blood samples. The associations of members of the Enterobacteriaceae with S. aureus were 2.4 times more frequent than associations of the Enterobacteriaceae with S. epidermidis, indicating a development of the polymicrobial infection and, accordingly, the development of purulent stage of acute pyelonephritis. Conclusion. For the diagnosis of acute pyelonephritis, we suggest to use an additional criterion, which consists in applying of FISH method for the detection and simultaneous identification ofbacteria that were adhered on the surface of erythrocytes and located intracellularly. This method may allow for the differential diagnosis of serous and purulent stages of acute pyelonephritis.
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作者简介
E. Shchuplova
Institute of Cellular and Intracellular Symbiosis, Ural Branch of the RAS
Email: Khanina83@yandex.ru
Ph.D. in Biology, senior researcher
M. Kuzmin
Institute of Cellular and Intracellular Symbiosis, Ural Branch of the RAS; Orenburg Regional Clinical Hospital
Email: mdk-49@mail.ru
MD, the Honored Physician of the Russian Federation and the Republic of Mordovia
参考
- Плеханов В.Н. О трудностях диагностики гнойно-деструктивныхформ острого пиелонефрита у мужчин молодого возраста. Вестник РУДН, серия Медицина. 2010;1:172-177
- Mahmood T., Puckrin RSugar L., Naimark D. Staphylococcus-associated glomerulonephritis mimicking henoch-schonlein purpura and cryoglobulinemic vasculitis in a patient with an epidural abscess: a case report and brief review of the literature. Canadian Journal of Kidney Health and Disease. 2018;5:1-6.
- Stamm W.E. Protocol for diagnosis ofurinary tract infection: reconsidering the criterion for significant bacteriuria. Urology. 1988;32(2 Suppl.):6-12.
- Weidner W., Ludwig M., Weimar B., Rau W. Rational diagnostic steps for acute pyelonephritis with special consideration of ultrasound and computed tomography. Int Journal Antimicrobiol Agents. 1999;11(3- 4):257-259.
- Синякова Л.А. Рецидивирующие инфекции нижних мочевых путей: сложности упрощенной диагностики. Урология сегодня.2013;1:18-21
- Аляев Ю., Газимиев М., Еникеев Д. Современные аспекты диагностики острого пиелонефрита. Врач. 2009;6:76-78
- Белый Л.Е. Неотложная урология: Руководство для врачей. М.: ООО «Медицинское информационное агентство». 2011: 472
- Щуплова Е.А., Черкасов С.В., Плотников А.О. Применение метода FISH для выявления бактерий, локализованных на поверхности и внутри эритроцитов. Клиническая лабораторная диагностика. 2017;62(7):431-435
- Руднов В.А. Сепсис: современные подходы к диагностике и интенсивной терапии. Вестник анестезиологии и реаниматологии. 2010;7(1):48-57
- Гаврилов С.Н., Скачкова Т.С., Шипулина О.Ю., Савочкина Ю.А., Шипулин Г.А., Малеев В.В. Современные молекулярно-генетические методы, используемые для этиологической диагностики сепсиса. Журнал микробиологии, эпидемиологии и иммунобиологии. 2016;2:91-99
- Вайнберг З.С. Неотложная урология. М.: Московский рабочий. 1997: 206
- Плеханов А.Н., Дамбаев А.Б. Инфекция мочевых путей: эпидемиология, этиология, патогенез, факторы риска, диагностика (обзор литературы). Бюллетень ВСНЦ СО РАМН. 2016; 1(107): 70-74
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