FemtoScan Online: image processing and filtering

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Abstract

The unique capabilities of the atomic force microscope (AFM), including super-high-resolution imaging, nanomanipulation, and the ability to operate under physiological conditions, have opened up exciting research opportunities in biology and biomedicine. AFM imaging has helped reveal the fine structure of bacterial cell walls at the nanoscale and how they are altered by antimicrobial treatment. This paper discusses the functions of FemtoScan Online software in relation to processing probe microscopy images, improving image quality reducing noise and increasing the informativeness of data.

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About the authors

A. I. Akhmetova

Lomonosov Moscow State University, Physical department; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-5115-8030

Cand. of Sci. (Physics and Mathematics), Leading Specialist

Russian Federation, Moscow; Moscow

D. I. Yaminsky

Lomonosov Moscow State University, Physical department

Email: yaminsky@nanoscopy.ru
ORCID iD: 0009-0009-6370-7496

Post Graduate

Russian Federation, Moscow

I. V. Yaminsky

Lomonosov Moscow State University, Physical department; Advanced Technologies Center

Author for correspondence.
Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8731-3947

Doct. of Sci. (Physics and Mathematics), Prof., Director

Russian Federation, Moscow; Moscow

References

  1. Yaminsky I.V., Akhmetova A.I., Meshkov G.B. FemtoScan Online software and visualization of nanoobjects in high-resolution microscopy. NANOINDUSTRY. 2018. Vol. 11. No. 6(85). PP. 414–416. https://doi.org/10.22184/1993-8578.2018.11.6.414.416
  2. Akhmetova A.I., Yaminsky I.V., Sovetnikov T.O. FemtoScan Online: 3D visualization and processing of bionanoscopy data. NANOINDUSTRY. 2023. Vol. 16. No. 3-4. PP. 450–455. https://doi.org/10.22184/1993-8578.2023.16.7-8.450.455
  3. Filonov A.S., Yaminsky I.V., Akhmetova A.I., Meshkov G.B. FemtoScan Online! Why he? NANOINDUSTRY. 2018. Vol. 84. No. 5. PP. 339–342. https://doi/org/10.22184/1993-8578.2018.84.5.336.342

Supplementary files

Supplementary Files
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1. JATS XML
2. Fig.1. Image of a defect on graphite and its Fourier image

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3. Fig.2. The original image X of potato virus and its Fourier image. A region is highlighted on the Fourier image that can be zeroed outward to remove all high frequencies in the image

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4. Fig.3. Obtained 2D image after high frequency zeroing

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5. Fig.4. Comparison of 3D images before and after high frequency removal using Fourier filtering

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6. Fig.5. 2D image X of potato virus and histogram of the height distribution of image points. The left peak corresponds to the height distribution of the pure substrate. The right small peak is the height distribution of the particles. The average value is about 8.5 nm

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Copyright (c) 2024 Akhmetova A.I., Yaminsky D.I., Yaminsky I.V.

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