Scanning capillary microscopy. Imaging and quantifying

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

The study of the morphology of objects and their mechanical characteristics makes it possible to detect the unique properties of cells and associate these features with development under normal conditions or in the presence of pathologies. To measure the surface of a sample, scanning capillary microscopy (SCM) uses an electrolyte-filled capillary with a nano-sized hole at the tip as a probe. The main advantage of SCM is the non-contact visualization of the biological objects topography in the natural environment – scanning is carried out without forceful contact of the probe tip with the sample surface. Additionally, SCM can be used to determine electrical charges at the solid-liquid interface. In this article, we describe the basics of SCM, its capabilities for imaging cells, and measuring the biomechanical properties of living samples.

Full Text

Restricted Access

About the authors

A. I. Akhmetova

Lomonosov Moscow State University, Physical department; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-5115-8030

Cand. of Sci. (Physics and Mathematics), Leading Specialist

Russian Federation, Moscow; Moscow

I. V. Yaminsky

Lomonosov Moscow State University, Physical department; Advanced Technologies Center

Author for correspondence.
Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8731-3947

Doct. of Sci. (Physics and Mathematics), Prof., Director

Russian Federation, Moscow; Moscow

References

  1. Akhmetova A.I., Sovetnikov T.O. et al. The heart of the capillary microscope. NANOINDUSTRY, 2023. Vol. 16. No. 7–8. PP. 444–448. https://doi.org/10.22184/1993-8578.2023.16.7-8.444.448
  2. Monzel C., Sengupta K. Measuring shape fluctuations in biological membranes. J. Phys. D: Appl. Phys., 49. 2016. Article 243002, https://doi.org/10.1088/0022-3727/49/24/243002
  3. Pellegrino M., Orsini P., Tognoni E. Membrane fluctuations of human red blood cells investigated by the current signal noise in scanning ion conductance microscopy, Micron, 2024. Vol. 181. P. 103635. https://doi.org/10.1016/j.micron.2024.103635
  4. Seifert J., Rheinlaender J. et al. Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy. Nat. Sci Rep. 2017. Vol. 7. No. 1. P. 4810. https://doi.org/10.1038/s41598-017-04999-6
  5. Nikolaev N.I., Müller T. et al. Changes in the stiffness of human mesenchymal stem cells with the progress of cell death as measured by atomic force microscopy. J. Biomech. 2014. Vol. 47. PP. 625–630. https://doi.org/10.1016/j.jbiomech.2013.12.004
  6. Su X., Zhou H. et al. Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy. Biol. Open. 2020. Vol. 9. https://doi.org/10.1242/bio.048108
  7. Meng H., Chowdhury T.T., Gavara N. The Mechanical interplay between differentiating mesenchymal stem cells and gelatin-based substrates measured by atomic force microscopy. Front. Cell Dev. Biol., 2021. Vol. 9. https://doi.org/DOI 10.3389/fcell.2021.697525
  8. Docheva D., Padula D. et al. Researching into the cellular shape, volume and elasticity of mesenchymal stem cells, osteoblasts and osteosarcoma cells by atomic force microscopy. J. Cell. Mol. Med., 2008. Vol. 12. PP. 537–552. https://doi.org/10.1111/j.1582-4934.2007.00138.xopen_in_new
  9. Nozawa K., Zhang X. et al. Topographical evaluation of human mesenchymal stem cells during osteogenic differentiation using scanning ion conductance microscopy, Electrochimica Acta. 2023. Vol. 449. P. 142192. https://doi.org/10.1016/j.electacta.2023.142192
  10. Muhammed Y., Lazenby R.A. Scanning ion conductance microscopy revealed cisplatin-induced morphological changes related to apoptosis in single adenocarcinoma cells. Anal Methods. 2024. Vol. 16. No. 4. PP. 503–514. https://doi.org/DOI 10.1039/d3ay01827j
  11. Wang D., Woodcock E. et al. Exploration of individual colorectal cancer cell responses to H2O2 eustress using hopping probe scanning ion conductance microscopy, Science Bulletin. 2024. https://doi.org/10.1016/j.scib.2024.04.004
  12. Honda K., Yoshida K. et al. In situ visualization of LbL-assembled film nanoscale morphology using scanning ion conductance microscopy, Electrochimica Acta. 2023. Vol. 469. P. 143152. https://doi.org/10.1016/j.electacta.2023.143152
  13. Akhmetova A.I., Sovetnikov T.O. et al. Scanning capillary microscopy in the study of the effect of cytotoxic agents on the biomechanical and physicochemical properties of tumor cells. Pharmaceutical Chemistry Journal. 2022. https://doi.org/10.1007/s11094-022-02770-4
  14. Sovetnikov T.O., Akhmetova A.I. et al. Scanning probe microscopy in assessing blood cells roughness. Bio-Medical Engineering. 2023. https://doi.org/10.1007/s10527-023-10253-3
  15. Actis P., Tokar S. et al. Electrochemical nanoprobes for single-cell analysis. ACS Nano. 2014. Vol. 8. No. 1. PP. 875–884. https://doi.org/10.1021/nn405612q
  16. Sovetnikov T.O., Akhmetova A.I. et al. Characteristics of the use of scanning capillary microscopy in biomedical research. Bio-Medical Engineering. 2023. Vol. 57. No. 4. PP. 250–253. https://doi.org/10.1007/s10527-023-10309-4

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig.1. Human embryonic stem cells (H9 lineage): 2D and 3D view

Download (224KB)
Indexing metadata
3. Fig.2. Mechanical system of FemtoScan Xi scanning capillary microscope. Precision movement range in X, Y, Z axes – 50 µm, 50 µm, 30 µm; movement accuracy – 0.05 nm. The range of smooth automated movement in X and Y axes is 12 mm. Built-in inverted optical system of sample observation with automated focusing system is presented

Download (121KB)
Indexing metadata

Copyright (c) 2024 Akhmetova A.I., Yaminsky I.V.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies