Originality without privilege: comparative analysis of electroformed and standard nitrocellulose membranes in melatonin immunoassays

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Abstract

Electrospinning enables manufacturing of polymer membranes composed of nanofibres. These membranes find application as filters, wound coatings, and tissue engineering scaffolds. They are also considered promising substrates for immunoassays. Despite the scientific community’s keen interest in the immunoassays based on electrospun membranes, a direct comparison with membranes formed through alternative technologies has not been conducted to date. In this study, we performed such a comparison and demonstrated that the detection of melatonin via enzyme-linked immunosorbent assay (ELISA) is virtually identical on the electrospun membranes and the conventional commercially available membranes.

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About the authors

S. V. Zamalutdinova

Lomonosov Moscow State University, Biological Department

Author for correspondence.
Email: podgorodova.sofya@yandex.ru
ORCID iD: 0009-0006-9510-1868

Junior Researcher

Russian Federation, Moscow

P. A. Petrova

Lomonosov Moscow State University, Biological Department

Email: podgorodova.sofya@yandex.ru
ORCID iD: 0009-0004-7933-0594

Junior Researcher

Russian Federation, Moscow

A. A. Ramonova

Lomonosov Moscow State University, Biological Department

Email: podgorodova.sofya@yandex.ru
ORCID iD: 0000-0002-3081-4721

Junior Researcher

Russian Federation, Moscow

D. V. Bagrov

Lomonosov Moscow State University, Biological Department

Email: podgorodova.sofya@yandex.ru
ORCID iD: 0000-0002-6355-7282

Cand. of Sci. (Physics and Mathematics), Lead Researcher

Russian Federation, Moscow

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Supplementary files

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2. Fig.1. Diagram of electroforming (electrospinning). The polymer solution is in a syringe, the needle is located in opposite to the conductive plate (collector). The nanofibres are formed by evaporation of the solvent and solidification of the polymer solution jet

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3. Fig.2. Images of membranes obtained by SEM method. a, c – electroformed membranes; b, d – conventional membranes. The scale mark is 5 µm (a, b) and 10 µm (c, d)

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4. Fig.3. EFA results. a – EFA calibration curve for MT obtained in a conventional plate; colourimetric detection was used; b – schematic of the vacuum clamp for dot-blotting; c – calibration curves of ELISA on MT obtained by dot-blotting; chemiluminescent detection was used; d – scheme of membrane placement in the clamp

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Copyright (c) 2024 Zamalutdinova S.V., Petrova P.A., Ramonova A.A., Bagrov D.V.

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