Scanning capillary microscopy as a tool for nanocapillary printing

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Controlled manipulation of cultured cells and local delivery of macromolecules and substances are still unsolved problems in experimental biology. Intracellular injection of various therapeutic agents, including biologics and supramolecular agents, is difficult due to natural biological barriers required to protect the cell. Efficient delivery of nucleic acids, proteins, peptides and nanoparticles is critical for clinical implementation of new technologies that can benefit the treatment of diseases using gene and cell therapy. Using a capillary, it is possible to locally apply a desired substance to a cell or even introduce it into the cell, and then evaluate its effect on morphology using scanning capillary microscopy (SCM) tools. These capabilities make the capillary microscopy method promising for biomedical purposes.

Full Text

Restricted Access

About the authors

A. I. Akhmetova

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-5115-8030

Cand. of Sci. (Physics and Mathematics), Researcher

Lomonosov Moscow State University, Physical Department

Russian Federation, Moscow; Moscow

T. O. Sovetnikov

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-6541-8932

Postgraduate, Engineer, Leading Engineer

Lomonosov Moscow State University, Physical Department

Russian Federation, Moscow; Moscow

A. D. Terentev

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0009-0009-1528-5284

Postgraduate, Programmer

Lomonosov Moscow State University, Physical Department

Russian Federation, Moscow; Moscow

I. V. Yaminsky

Lomonosov Moscow State University; Advanced Technologies Center

Author for correspondence.
Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8731-3947

Doct. of Sci. (Physics and Mathematics), Prof., Director General

Lomonosov Moscow State University, Physical Department

Russian Federation, Moscow; Moscow

References

  1. Elnathan R. et al. Engineering vertically aligned semiconductor nanowire arrays for applications in the life sciences. Nano Today 9. 2014. PP. 172–196. https://doi.org/10.1016/j.nantod.2014.04.001
  2. Tay A. The benefits of going small: nanostructures for mammalian cell transfection. ACS Nano. 2020. Vol. 14. PP. 7714–7721. https://doi.org/10.1021/acsnano.0c04624
  3. He G. et al. Nanoneedle platforms: the many ways to pierce the cell membrane. Adv. Funct. Mater. 2020. Vol. 30. P. 1909890. https://doi.org/10.1002/adfm.201909890
  4. Liu R. et al. High density individually addressable nanowire arrays record intracellular activity from primary rodent and human stem cell derived neurons. Nano Lett. 2017. Vol. 17. PP. 2757–2764. https://doi.org/10.1021/acs.nanolett.6b04752
  5. Abbott J. et al. Optimizing nanoelectrode arrays for scalable intracellular electrophysiology. Acc. Chem. Res. 51. 2018. PP. 600–608. https://doi.org/10.1021/acs.accounts.7b00519
  6. Chen Y. et al. Emerging roles of 1D vertical nanostructures in orchestrating immune cell functions. Adv. Mater. 32. 2020. P. e2001668. https://doi.org/10.1002/adma.202001668
  7. Wang Z. et al. Interrogation of cellular innate immunity by diamond-nanoneedle-assisted intracellular molecular fishing. Nano Lett. 2015. Vol. 15. PP. 7058–7063. https://doi.org/10.1021/acs.nanolett.5b03126
  8. Leitao S.M. et al. Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds. Nat. Nanotechnol. 2023. Vol. 18. PP. 1078–1084. https://doi.org/10.1038/s41565-023-01412-4
  9. Varongchayakul N. et al. Single-molecule protein sensing in a nanopore: a tutorial. Chem. Soc. Rev. 47. 2018. PP. 8512–8524. https://doi.org/10.1039/c8cs00106e
  10. Chau C.C. et al. Macromolecular crowding enhances the detection of DNA and proteins by a solid-state nanopore. Nano Lett. 20. 2020. PP. 5553–5561. https://doi.org/10.1021/acs.nanolett.0c02246
  11. Confederat S. et al. Next-generation nanopore sensors based on conductive pulse sensing for enhanced detection of nanoparticles. Small. 2023. https://doi.org/10.1002/smll.202305186
  12. Chau C.C. et al. Single molecule delivery into living cells. Nat Commun. 2024. Vol. 15. No. 1. P. 4403. https://doi.org/10.1038/s41467-024-48608-3
  13. O’Connell M.A. et al. Positionable vertical microfluidic cell based on electromigration in a theta pipet. Langmuir. 2014. Vol. 30. PP. 10011–10018. https://doi.org/10.1021/la5020412
  14. McKelvey K. et al. Meniscus confined fabrication of multidimensional conducting polymer nanostructures with scanning electrochemical cell microscopy (SECCM). Chem. Comm. 2013. Vol. 49. PP. 2986–2988. https://doi.org/10.1039/c3cc00104k
  15. Pastre D. et al. Characterization of AC mode scanning ion-conductance microscopy. Ultramicroscopy. 2001. Vol. 90. PP. 13–19. https://doi.org/10.1016/s0304-3991(01)00096-1
  16. Rheinlander J., Schaffer T.E. An accurate model for the ion current–distance behavior in scanning ion conductance microscopy allows for calibration of pipet tip geometry and tip–sample distance. Anal. Chem. 2017. Vol. 89. No. 21. PP. 11875–11880. https://doi.org/10.1021/acs.analchem.7b03871
  17. Lukashenko S.Y. et al. Behavioral features of the approach curve of a scanning ion-conductance microscope. J. Surf. Investig. 2023. Vol. 17. PP. 585–591. https://doi.org/10.1134/S1027451023030096
  18. Sovetnikov T.O. et al. Characteristics of the use of scanning capillary microscopy in biomedical research. Bio-Medical Engineering 57. 2023. Vol. 4. PP. 250–253. https://doi.org/10.1007/s10527-023-10309-4
  19. Rodolfa K.T. et al. Two-Component Graded Deposition of Biomolecules with a Double-Barreled Nanopipette. Angewandte Chemie International Edition. 2005. Vol. 44. No. 42. PP. 6854–6859. https://doi.org/10.1002/anie.200502338
  20. Akhmetova A.I. et al. Scanning capillary microscopy for biological applications. NANOINDUSTRY. 2024. Vol. 17. No. 6. https://doi.org/10.22184/1993-8578.2024.17.6.364.370

Supplementary files

Supplementary Files
Action
1. JATS XML
2. Fig.1. Equivalent circuit diagram to explain the drop in the magnitude of the recorded ionic current between Ag/AgCl electrodes in the capillary and in the medium as the nanoparticle passes through the capillary openings. Rp reflects the resistance of the capillary medium, ri – inner radius of the capillary, rчастицы – radius of the particle, α – capillary apex angle

Download (71KB)
3. Fig.2. Schematic diagram of the electrolyte droplet deposition process on a wetted substrate

Download (112KB)
4. Fig.3. A drop at the tip of a capillary. The diameter of the drop is about 10 µm

Download (125KB)

Copyright (c) 2024 Akhmetova A.I., Sovetnikov T.O., Terentev A.D., Yaminsky I.V.