Living cell as an object in scanning probe microscopy

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Abstract

The mechanical properties of cells and tissues can reflect pathological conditions such as tumors, inflammation, and viral infections. Probe microscopy allows one to evaluate the mechanical properties of biological samples with minimal impact on the object of study. Advanced probe microscopy methods allow one to evaluate cell morphology and rigidity, measure elasticity, Young’s modulus, adhesion, and resistance to mechanical impact, and record force curves at a specific point. In addition, AFM allows one to perform noninvasive and nondestructive measurements with simple sample preparation in air and in an aqueous environment at room temperature, which significantly increases its advantage for studying biological functions.

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About the authors

A. I. Akhmetova

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-5115-8030

Physical Department, Cand. of Sci. (Physics and Mathematics), Senior Researcher

Russian Federation, Moscow; Moscow

I. V. Yaminsky

Lomonosov Moscow State University; Advanced Technologies Center

Author for correspondence.
Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8731-3947

Physical Department, Doct. of Sci. (Physics and Mathematics), Prof., Director General

Russian Federation, Moscow; Moscow

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2. Fig.1. 2d and 3d images of leukocytes in air. According to the section data, the cell height is 330 nm, the radius is 991 nm

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