Scanning probe microscopy of fibrosarcoma

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Abstract

Scanning capillary microscopy (SCM) has become a universal method for studying interactions in living cells and tissues. SCM finds successful application in biology and materials science in biophysical and electrochemical measurements. Initially, this type of microscopy was used mainly to record 3D morphology of cells in the natural environment, but soon the method began to develop due to the use of modified and multichannel capillaries, which made it possible to record active oxygen species near and inside the cell surface, evaluate deformation and other mechanical properties of the objects under study. Modern modifications of the SCM setup have made this method an important tool in bioanalytical, biophysical and materials science measurements. This paper presents a study of fibrosarcoma cells using the FemtoScan X Aion capillary microscope, developed on the basis of original electronics, mechanics and software systems.

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About the authors

A. I. Akhmetova

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-5115-8030

Cand. of Sci. (Physics and Mathematics), Senior Researcher, Leading Specialist, Physical Department

Russian Federation, Moscow; Moscow

T. O. Sovetnikov

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-6541-8932

Postgraduate, Engineer, Physical Department

Russian Federation, Moscow; Moscow

A. D. Terentiev

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0009-0009-1528-5284

Postgraduate, Programmer, Physical Department

Russian Federation, Moscow; Moscow

I. V. Yaminsky

Lomonosov Moscow State University; Advanced Technologies Center

Author for correspondence.
Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8731-3947

Doct. of Sci. (Physics and Mathematics), Prof., Director, Physical Department

Russian Federation, Moscow; Moscow

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Supplementary files

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2. Fig.1. Compact version of the scanning capillary microscope

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3. Fig.2. Image from the inbuilt camera of the optical microscope, the light spot on the frame is a capillary in the process of bringing HT1080 cells to the sample

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4. Fig.3. Oscilloscope measures the ionic current magnitude between the electrode in the capillary and the electrode in the Petri dish in the 3/10 kHz frequency bandwidth

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5. Fig.4. Sample capillary approach menu

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6. Fig.5. Standard parameters for scanning

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7. Fig.6. Characteristic morphology of HT1080 cells. Scanning capillary microscopy

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Copyright (c) 2025 Akhmetova A.I., Sovetnikov T.O., Terentiev A.D., Yaminsky I.V.