Development of a biocompatible multi-electrode cell for studying living neuronal networks using combined scanning capillary microscopy

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Abstract

An innovative biocompatible multielectrode cell integrating neuronal electrical activity recording technologies with high-resolution scanning capillary microscopy capabilities has been developed and characterized. The proposed design includes an ITO-coated glass substrate with laser-patterned electrodes insulated with a 180 nm thick parylene layer and functionalized copper-gold microelectrodes with memristor properties. The prototype demonstrates the advantages of simultaneous recording of neuronal electrical activity and morphological changes, opening up new possibilities for studying neuronal plasticity, structural distribution of nervous tissue, and screening of neuroactive compounds.

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About the authors

O. V. Ivanov

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0003-2765-2116

Master

Russian Federation, Moscow; Moscow

A. I. Akhmetova

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-5115-8030

Cand. of Sci. (Physics and Mathematics), Senior Researcher, Leading Specialist

Russian Federation, Moscow; Moscow

I. V. Yaminsky

Lomonosov Moscow State University; Advanced Technologies Center

Author for correspondence.
Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8731-3947

Doct. of Sci. (Physics and Mathematics), Prof., Director

Russian Federation, Moscow; Moscow

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2. Fig.1. Biointerface cell model. The bottom layer is 1 mm glass, the middle layer is a 200 nm ITO coating, and a 180 nm thick layer of parylene is deposited on it. Metal electrodes are shown on the parylene layer

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3. Fig.2. Integration of the multielectrode cell with the perfusion system and functional holes for electrodes of the scanning capillary microscope

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Copyright (c) 2025 Ivanov O.V., Akhmetova A.I., Yaminsky I.V.