Scanning probe microscopy in the study of insect neuronal activity: prospects and methodological approaches

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Abstract

The paper discusses the possibilities of using scanning probe microscopy (SPM) to study the neural activity of insects, including recording action potentials and visualizing the morphological features of the nervous system. Particular attention is paid to the advantages of using insects such as mosquitoes as model objects for neurophysiological research. Modern methods of recording neural impulses are discussed, including intracellular and extracellular recording, the local potential fixation method (patch clamp), as well as a comparison of action potential parameters in insects and humans. The prospects for integrating SPM with optogenetics and calcium imaging to obtain a comprehensive understanding of the functional activity of neurons in vivo are presented.

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About the authors

I. V. Yaminsky

Lomonosov Moscow State University; Advanced Technologies Center

Author for correspondence.
Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8731-3947

Doct. of Sci. (Physics and Mathematics), Physical Department, Prof., Director General

Russian Federation, Moscow; Moscow

References

  1. Yang R., Zaheri A., Gao W., Hayashi C., Espinosa H.D. AFM Identification of Beetle Exocuticle: Bouligand Structure and Nanofiber Anisotropic Elastic Properties. Adv. Funct. Mater. 2017. Vol. 27. P. 1603993. https://doi.org/10.1002/adfm.201603993
  2. Stavenga D.G., Foletti S., Palasantzas G., Arikawa K. Light on the moth-eye corneal nipple array of butterflies. Proc. R. Soc. B. 2006. PP. 273661–273667. https://doi.org/10.1098/rspb.2005.3369
  3. Dorkenwald S., Matsliah A., Sterling A.R. et al. Neuronal wiring diagram of an adult brain. Nature. 2024. Vol. 634. PP. 124–138. https://doi.org/10.1038/s41586-024-07558-y
  4. Novak P., Li C., Shevchuk A. et al. Nanoscale live-cell imaging using hopping probe ion conductance microscopy. Nat Methods. 2009. Vol. 6. PP. 279–281. https://doi.org/10.1038/nmeth.1306

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2. Fig.1. Optical photograph of a representative of the class of invertebrate arthropods – fly. Object for research by scanning probe microscopy. Image size 5 × 3.8 mm2. The diameter of the fly’s eye is about 0.28 mm

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