Effect of a new blood substitute rheoambrasol on indicators of endogenous intoxication in nitrite-induced methemoglobinemia
- Authors: Khakimova D.Z1, Shevchenko L.I.2, Karimov K.Y.2, Khuzhahmedov Z.D3
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Affiliations:
- Clinic of the Tashkent Medical Academy
- Republican Specialized Scientific and Practical Medical Center for Hematology of the Ministry of Health of the Republic of Uzbekistan
- Molecular Genetic Laboratory “GenoTexnologiya"
- Issue: Vol 29, No 13 (2022)
- Pages: 115-119
- Section: Articles
- URL: https://journals.eco-vector.com/2073-4034/article/view/321141
- DOI: https://doi.org/10.18565/pharmateca.2022.13.115-119
- ID: 321141
Cite item
Abstract
Background. The introduction of chemicals into various types of industry, everyday life, crop production and science provides a background for the development of methemoglobinemia. As a preparation capable of restoring cell metabolism under conditions of hypoxia, we offer the blood substitute rheoambrasol with antihypoxic, antioxidant and detoxification efficacy, developed at the Republican Specialized Scientific and Practical Medical Center for Hematology of the Ministry of Health of the Republic of Uzbekistan. Objective. Evaluation of the effect of rheoambrasol on the indicators of endogenous intoxication in nitrite-induced methemoglobinemia. Methods. Toxic methemoglobinemia in rats was created by daily administration of sodium nitrite at a dose of 50 mg/kg for 30 days. During the setting of the model of methemoglobinemia, the methemoglobin (metHb) level was determined one hour after the administration of sodium nitrite, on the 3rd, 15th and 30th days. On the 30th day after the reproduction of methemoglobinemia, rats were treated with blood substitutes at a dose of 10 ml/kg for 5 days: new blood substitute rheoambrasol in the experimental group and rheopolyglucin in the comparison group. Indicators of endogenous intoxication were determined during the experiment. Results. The study showed that the use of rheoambrasol effectively reduces the metHb level and restores the indicators of endogenous intoxication in experimental methemoglobinemia to the initial values. Compared with the results after the use of rheopolyglucin, one hour after the administration of the new blood substitute rheoambrasol, the sorption capacity of erythrocytes (SCE) was lower by 28.9% (p<0.05), plasma middle molecules (MMpl) - by 29.0% (p<0.05), plasma oligopeptides (OPpl) - by 20.6% (p<0.05), middle molecules (MMer) - by 22.5% (p<0.01), erythrocyte oligopeptides (OPer) - by 25.5% (p<0.01), plasma toxemia index(TIpl) - by 43.6% (p<0,01), erythrocyte toxemia index (Tier) - by 41.8% (p<0.01), intoxication index (II) - by 42.8% (p<0.01), MMpl/MMer - by 8.4% (p<0.05). Conclusions. The new blood substitute rheoambrasol restores metHb level in long-term nitrite-induced methemoglobinemia. Reoambrasol has a pronounced detoxification effect, which manifested itself in a decrease in endogenous intoxication with prolonged nitrite-induced methemoglobinemia and indicators of endogenous intoxication were restored almost to the initial level.
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About the authors
D. Z Khakimova
Clinic of the Tashkent Medical Academy
Larisa I. Shevchenko
Republican Specialized Scientific and Practical Medical Center for Hematology of the Ministry of Health of the Republic of Uzbekistan
Email: moriturus1958@mail.ru
Cand. Sci. (Biol.), Head of the Research Grant, Head of the Laboratory of Blood Substitutes
Kh. Ya Karimov
Republican Specialized Scientific and Practical Medical Center for Hematology of the Ministry of Health of the Republic of Uzbekistan
Zh. D Khuzhahmedov
Molecular Genetic Laboratory “GenoTexnologiya"
References
- Будников Д.А. и др. Нитраты в воде источников нецентрализованного питьевого водоснабжения республики Беларусь: оценка влияния на состояние здоровья детей в возрасте до 6 лет. Здоровье и окружающая среда. 2012;21:309 18
- Тулишевская Ю.В. Осведомленность населения об отравлениях продуктами животного и растительного происхождения. 2021. С. 330-37
- Katabami K., Hayakawa M., Gando S. Severe methemoglobinemia due to sodium nitrite poisoning. Case reports in emergency medicine. 2016. Р 1-3
- Гуляева И.Л., Сергеева С.А., Черешнев В.А. Эритроциты как модель для изучения патогенеза и оценки эффективности терапии хронической нитритной интоксикации. Вестник уральской медицинской академической науки. 2010;4:58-61.
- Шевченко Л.И., Каримов Х.Я., Рахманбердиева РК., Сагдуллаев Ш.Ш. Полифункциональный кровезаменитель гемо-динамического действия. Патент IAP 06029 от 28.10.2015. Расмий ахборотнома. 2019;11(223):59-9
- Черныш А.М., Козлова Е.К., Мороз В.В. и др. Влияние антиоксиданта на основе янтарнойкислоты на превращение метгемоглобина в оксигемоглобин in vitro. Общая реаниматология. 2018;2:46-59
- Игбаев Р.К. Экспериментальная коррекция про-оксидантно-антиоксидантного равновесия в условиях гипоксии и токсической метгемогло-бинемии. Дисс. канд. мед. наук. Уфа, 2006
- Козаева Э.Г. Влияние экспериментальной нитритной и нитратной интоксикации на функции почек у крыс. Дисс. канд. мед. наук. Владикавказ, 2011
- Волчков А.Б. Ерыкалов М.Ю. Любимова Л.В. Способ определения концентрации метгемоглобина в крови. Пат. 2186397. Российская федерация, МПК7 G01 №33/72, G01 №33/49; заявитель и патентообладатель Волчков А.Б. №2000114327/14; заявл. 05.06.2000; опубл. 27.07.2002
- Давыдов В.М., Ованесов Е.Н., Сецко И.В. К вопросу о точности фотометрического определения концентрации гемоглобина. Электронный ресурс: http://www.clinlab.ru/main/publicat/refer21.htm
- Черныш А.М. и др. Способ определения концентраций гемоглобина и его производных в крови. 2018. С. 1-17
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