Study of the synthetic function of fibroblasts after exposure to collagen preparations

Background. Most of the works published in recent years have demonstrated the positive effect of collagen on clinical signs of skin aging. The use of collagen as a dietary supplement has a long history; however, only a few studies have looked at injectable forms of collagen in the cellular and molecular biology of skin cells, which could shed light on the results of clinical improvement. This study is devoted to the assessment of gene expression after exposure to collagen preparations on fibroblast culture.

Objective. Evaluation of the gene expression (collagen type I (COL1), elastin (ELN), matrix metalloproteinase type 1 (MMP1), matrix metalloproteinase type 3 (MMP3) and verzican (VCAN)) after exposure to collagen preparations (Collost micro, Linerase, Nithya, Collapro30+, Collapro45+ and Collapro55+).

Methods. Gene expression was measured using real-time reverse transcription polymerase chain reaction (RT-rtPCR).

Results. For Linerase, higher COL1 gene expression was observed after 48 hours compared to other preparations. It should be noted that there was no MMP1 gene expression after 24 hours in all samples except for samples treated with Collapro30+ and Collapro55+. After 48 hours of cell treatment, the expression of the MMP1 gene was detected only in the sample treated with Linerase. The results of pairwise comparison of the differences in expression between the groups for the MMP3 gene (24 hours and 48 hours after incubation) were not revealed. Also, in the samples treated with Collapro30+, Collapro45+ and Collapro55+, verzican is expressed at a higher level on the first day of the study. On the second day, the VCAN gene is expressed at a higher level in Collapro45+ and Linerase.