The effectiveness of the new blood substitute rheoambrasol in experimental toxic hypoxia

Objective. Assessment of the effect of the new blood substitute rheoambrasol on the hypoxia-inducible factor (HIF-1a) level, lipid peroxidation (LPO) and the antioxidant system in toxic hypoxia. Methods. The model of toxic hypoxia was performed in outbred male rats. In the blood of experimental animals, the HIF-1α level, biochemical parameters using HUMAN test systems (HUMAN Gmbh, Germany), the intensity of lipid peroxidation by the level of malondial-dehyde, the activity of enzymes of the antioxidant system (AOS): catalase, superoxide dismutase, glutathione reductase and glutathione peroxidase were determined. Results. The action of rheoambrasol was clearly manifested and showed that the new blood substitute restores biochemical parameters and reduces the HIF-1α level and the balance of the LPO/AOS system in toxic hypoxia more effectively than rheopolyglucin. The biologically active composition, including a polysaccharide and a bioenergetic substrate, has good antihypoxic, antioxidant, detoxifying, membrane protective effects, which makes it possible to recommend it for various hypoxic toxic conditions. Conclusion. The created new blood substitute rheoambrasol, containing a polysaccharide and a bioenergetic substrate, restored the structural and functional parameters of the liver, which was manifested by a slowdown in the process of cytolysis, cholestasis in toxic hypoxia. The new blood substitute has an antihypoxic effect, reducing the level of hypoxia-inducible factor HIF-1α by 4.5 times (pi<0.05). Rheoambrasol has an antioxidant effect, reduces the intensity of LPO processes and restores the activity of AOS enzymes.

rheoambrasol, toxic hypoxia, hypoxia-inducible factor (HIF-1α), lipid peroxidation, antioxidant system