ANALYSIS AND SPREAD OF GYRA GENE MUTATIONS ENCODING DRUG RESISTANCE TO FLUOROQUINOLONES IN M. TUBERCULOSIS
- Authors: SALINA T.Y.1, MOROZOVA T.I.1,2
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Affiliations:
- Razumovsky Saratov State Medical University, Ministry of Health of Russia
- Saratov Regional Clinical Tuberculosis Dispensary
- Issue: No 6 (2013)
- Pages: 72-74
- Section: Articles
- URL: https://journals.eco-vector.com/2226-6976/article/view/277272
- ID: 277272
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Abstract
To investigate the spectrum and spread of gyrA gene mutations, 69 sputum samples from patients with active pulmonary tuberculosis were examined using biological microchips (BIOCHIP-IMB, Moscow). M. tuberculosis DNA was isolated in 60 (86.9%) people. gyrA gene mutations were found in 12 (20%) patients; the mutations were established in codons 88, 90, 91, and 94. Six (50%) showed a combination of two or more mutations. Twenty-nine (48.3%) persons had a codon 95 mutation that was caused by natural gene polymorphism and failed to result in resistance. Thus, the patients living in the Saratov Region are recorded to have a high level of gyrA gene mutations encoding drug resistance to fluoroquinolones.
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About the authors
Tatyana Y. SALINA
Razumovsky Saratov State Medical University, Ministry of Health of Russia
Email: SalinaTU@rambler.ru
T. I. MOROZOVA
Razumovsky Saratov State Medical University, Ministry of Health of Russia; Saratov Regional Clinical Tuberculosis Dispensary
Email: dispans@san.ru
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