Actual aspects of studying Candidatus Rickettsia tarasevichiae


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Abstract

Objective. To analyze of the data of investigating novel Candidatus Rickettsia tarasevichiae species. Materials and methods. 382 Ixodes persulcatus ticks, 4 Candidatus R. tarasevichiae strains, and 276 patient serum samples were used in the investigation. Rickettsiae were isolated in Vero cell cultures using shell vial technology. Infection was experimentally reproduced in male guinea pigs. The rickettsiae were identified by PCR, followed by sequence analysis. The patient serum samples were analyzed for IgM and IgG antibodies against Candidatus R. tarasevichiae, by using enzyme immunoassay. Results. I. persulcatus ticks were determined to be infected with this microorganism on the territory of Russia. Fragments of the ompA gene of Candidatus R. tarasevichiae were first revealed when examining the vectors taken from humans in the Omsk Region. The specific features of cultivation of Candidatus R. tarasevichiae strains were first investigated. This Rickettsia species causes flightless experimental infection, predominantly involving the cerebral vessels. Conclusion. In the area of I. persulcatus ticks, the principal host for Candidatus R. tarasevichiae, some fevers of unestablished etiology may be caused by this Rickettsia species after tick suction, which must be taken into account when making a differential diagnosis.

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About the authors

N. V Rudakov

Omsk Research Institute of Natural Focal Infections, Federal Service for Supervision of Consumer Rights Protection and Human Welfare; Omsk State Medical University, Ministry of Health of Russia

Email: rickettsia@mail.ru

S. N Shpynov

Omsk Research Institute of Natural Focal Infections, Federal Service for Supervision of Consumer Rights Protection and Human Welfare; Omsk State Medical University, Ministry of Health of Russia

Email: stan63@inbox.ru

I. E Samoylenko

Omsk Research Institute of Natural Focal Infections, Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: samoilenko.irinasamoylenko@yandex.ru

L. V Kumpan

Omsk Research Institute of Natural Focal Infections, Federal Service for Supervision of Consumer Rights Protection and Human Welfare; Omsk State Medical University, Ministry of Health of Russia

Email: ludmilavirus@mail.ru

A. N Kolomeets

Omsk Research Institute of Natural Focal Infections, Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: arbitasfoc2@gmail.com

N. V Abramova

Omsk Research Institute of Natural Focal Infections, Federal Service for Supervision of Consumer Rights Protection and Human Welfare; Omsk State Medical University, Ministry of Health of Russia

Email: abnata83@mail.ru

T. A Reshetnikova

Omsk Research Institute of Natural Focal Infections, Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: tareshetnikova@mail.ru

N. A Okolelova

Omsk Research Institute of Natural Focal Infections, Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: okolelova-natalya@mail.ru

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