Use of immunochromatographic tests in a laboratory diagnostic algorithm for influenza


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Objective. To estimate the diagnostic significance of identifying the antigens of influenza A and B viruses by immunochromatography (ICG) versus that of detecting influenza virus nucleic acids (NAs) by a real-time polymerase chain reaction (RT-PCR) assay, as well as to determine the optimal procedure for their use in the diagnosis of influenza. Subjects and methods. Nasopharyngeal swabs from 211 children with influenza-like symptoms were concurrently examined by ICG and RT-PCR for NAs of influenza viruses and 19 other pathogen species of acute respiratory diseases. Results. 85.8% of the children were found to have NAs in the pathogens of acute respiratory infections; influenza was confirmed in 62.6%. ICG was used to detect influenza virus antigens in 31.3% of the children. The diagnostic sensitivity of ICG for influenza A(H3N2) and B viruses was 58 and 20%, respectively; its diagnostic specificity was 97.5 and 97.7%. Conclusion. The negative result of ICG cannot be an exception to the diagnosis of influenza due to the low sensitivity of this technique. Examinations on 1-2 days of the disease allow one to increase the sensitivity of ICG for the diagnosis of influenza A; this technique is minimally informative for the diagnosis of influenza B. It is advisable to use RT-PCR to rule out the diagnosis of influenza and to determine respiratory infection of another etiology.

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Sobre autores

S. Yatsyshina

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: svetlana.yatsyshina@cmd.su
Cand. Biol. Sci., Senior Researcher, Department of Molecular Diagnosis and Epidemiology 3a, Novogireevskaya St., Moscow 111123, Russia Telephone: +7(495)974-96-46, ext.2260

T. Kulichenko

National Medical Research Center for Children's Health, Ministry of Health of Russia

Email: Tkulichenko@yandex.ru
MD, Head, Department of Urgent Pediatrics, N ational Medical Research Moscow, Russia

I. Artemova

National Medical Research Center for Children's Health, Ministry of Health of Russia

Email: sko-inga@yandex.ru
Pediatrician, Department of U rgent Pediatrics, National Medical Research Moscow, Russia

O. Rybalka

National Medical Research Center for Children's Health, Ministry of Health of Russia

Email: olya.rybalka@yandex.ru
Pediatrician, Department of Urgent Pediatrics, National Medical Research Moscow, Russia

M. Elkina

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: mariyaelkina@yahoo.com
Junior Researcher, Department of Molecular Diagnostics and Epidemiology Moscow, Russia

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