First data on the prevalence of inherited chromosomally integrated Human betaherpesvirus 6A/B in Russia


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Abstract

Objective. To study the prevalence of inherited chromosomally integrated human herpesvirus 6A/B (iciHHV-6A/B) in Russia. Subjects and methods. A total of 262 apparently healthy people living in Moscow and its Region, as well as 3 members of one family were examined to establish the hereditary transmission of the virus. The investigators used diagnostic and exploratory research techniques, such as real-time PCR, Sanger sequencing, and massively parallel sequencing. Results. HHV- 6A/B DNA was detected in the blood of 5.7% (15/262) cases. The concentrations of HHV- 6A/B DNA were <1.0, 1.0-2.0, and >5.0 lg copies/105 cells in 12 (4.6%), 2 (0.8%), and 1 (0.4%) cases, respectively. PCR analysis of the hair follicles and nail plates verified iciHHV-6B status in one examinee. Interpretation of this case established the hereditary transmission in three generations: mother-daughter-grandson. Phylogenomic analysis showed that the Russian clinical endogenous isolates formed a unique monophilic branch within the clade that was represented mainly by iciHHV-6B isolates from Europe. Conclusion. The investigation provided preliminary data on the detection rate of inherited iciHHV-6A/B in Russia. The prevalence of inherited iciHHV-6B was 0.4% (1/262; 95% CI, 0-2.1); inherited iciHHV-6B was detected and confirmed by laboratory tests in 3 generations. Endogenous iciHHV-6A was not identified. Further large-scale studies are needed in Russia to understand the genetic diversity and geographic stratification of inherited iciHHV- 6A and iciHHV-6B.

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About the authors

Elvira A. Domonova

Central Research Institute of Epidemiology, Russian Federal Service for Surveillance of Consumer Rights Protection and Human WellBeing

Email: elvira.domonova@pcr.ms
Cand. Biol. Sci., Senior Researcher, Department of Molecular Diagnostics and Epidemiology Moscow, Russia

Olga Y. Silveistrova

Central Research Institute of Epidemiology, Russian Federal Service for Surveillance of Consumer Rights Protection and Human WellBeing

Junior Researcher, Department of Molecular Diagnostics and Epidemiology Moscow, Russia

Irina A. Goptar

Academician N.F. Izmerov Research Institute of Occupational Medicine

Email: probirka@list.ru
Cand. Chem. Sci., Senior Researcher, Laboratory of Postgenomic Technologies Moscow, Russia

Konstantin V. Kuleshov

Central Research Institute of Epidemiology, Russian Federal Service for Surveillance of Consumer Rights Protection and Human WellBeing

Email: konstantinkul@gmail.com
Cand. Biol. Sci., Senior Researcher, Department of Molecular Diagnostics and Epidemiology Moscow, Russia

Anastasia V. Nikiforova

Academician N.F. Izmerov Research Institute of Occupational Medicine

Email: anastasia@gmail.com
Junior Researcher, Laboratory of Postgenomic Technologies Moscow, Russia

Svetlana V. Matosova

Central Research Institute of Epidemiology, Russian Federal Service for Surveillance of Consumer Rights Protection and Human WellBeing

Email: matosova@cmd.su
Junior Researcher, Department of Molecular Diagnostics and Epidemiology Moscow, Russia

Olga Y. Shipulina

Central Research Institute of Epidemiology, Russian Federal Service for Surveillance of Consumer Rights Protection and Human WellBeing

Email: olga.shipulinа@pcr.ms
Cand. Sci. Med., Head, Subdivision of Molecular Diagnostic Methods, Department of Molecular Diagnostics and Epidemiology Moscow, Russia

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