The relationship between the clinical course of tropical malaria and the structural features of the Plasmodium falciparum genome
- Authors: Solovyev A.I1, Uskov A.N1,2, Gudkov R.V1, Dementyeva N.V3, Kapatsina V.A4, Komarov A.G1, Krutikova A.A3, Kovalenko A.N1, Laskin A.V1, Rakin A.I1
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Affiliations:
- S.M.Kirov Military Medical Academy, Ministry of Defense of the Russian Federation
- Pediatric Research and Clinical Center for Infectious Diseases, Federal Biomedical Agency
- Laboratory of Molecular Genetics, All-Russian Research Institute for Farm Animal Genetics and Breeding (Branch, L.K. Ernst Federal Research Center for Animal Husbandry)
- S.P. Botkin Clinical Infectious Diseases Hospital
- Issue: Vol 10, No 1 (2020)
- Pages: 67-74
- Section: Articles
- URL: https://journals.eco-vector.com/2226-6976/article/view/287463
- DOI: https://doi.org/10.18565/epidem.2020.10.1.67-74
- ID: 287463
Cite item
Open Access
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Abstract
Objective. To study the genotypic characteristics of Plasmodium falciparum that causes tropical malaria complicated by hemolytic anemia. Materials and methods. Two tropical malaria cases identified in Saint Petersburg hospitals in 2018 served as a material for clinical observations. In one case, the disease was severe and accompanied by the development of severe anemia; in the other case, it was uncomplicated. A polymerase chain reaction (PCR) assay was used for the investigation. The investigators used primers for the marker nucleotide sequences of the major virulence factor in P. falciparum (Plasmodium falciparum erythrocyte membrane protein 1 (PFEMP1)), encoding the structure of DBLla, DBLfi, CIDRla domains. Results. It was shown that in severe tropical malaria accompanied by the development of hemolytic anemia, the genetic markers of PFEMP1, which corresponded to the DBLla and DBLfi domains, were identified in the patient’s blood. In uncomplicated tropical malaria, these genetic markers were absent. It was suggested that there might be a relationship between the features of the P. falciparum genome and the clinical course of tropical malaria. The specific nucleotide sequences of the P. falciparum genome encoding the DBL1a and DBLfi domains of PFEMP1 are early predictors for the severe course of the disease. Conclusion. Detection of appropriate genetic markers in the blood of patients can be used in clinical practice to predict the development of tropical malaria.
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About the authors
A. I Solovyev
S.M.Kirov Military Medical Academy, Ministry of Defense of the Russian Federation
Email: solopiter@gmail.com
A. N Uskov
S.M.Kirov Military Medical Academy, Ministry of Defense of the Russian Federation; Pediatric Research and Clinical Center for Infectious Diseases, Federal Biomedical Agency
Email: aouskov@gmail.com
R. V Gudkov
S.M.Kirov Military Medical Academy, Ministry of Defense of the Russian Federation
Email: gudkoff@mail.ru
N. V Dementyeva
Laboratory of Molecular Genetics, All-Russian Research Institute for Farm Animal Genetics and Breeding (Branch, L.K. Ernst Federal Research Center for Animal Husbandry)
Email: dementevan@mail.ru
V. A Kapatsina
S.P. Botkin Clinical Infectious Diseases Hospital
Email: ingashi@mail.ru
A. G Komarov
S.M.Kirov Military Medical Academy, Ministry of Defense of the Russian Federation
Email: qwer.xo@mail.ru
A. A Krutikova
Laboratory of Molecular Genetics, All-Russian Research Institute for Farm Animal Genetics and Breeding (Branch, L.K. Ernst Federal Research Center for Animal Husbandry)
Email: anntim2575@mail.ru
A. N Kovalenko
S.M.Kirov Military Medical Academy, Ministry of Defense of the Russian Federation
Email: 9268754@mail.ru
A. V Laskin
S.M.Kirov Military Medical Academy, Ministry of Defense of the Russian Federation
Email: lar_vma@mail.ru
A. I Rakin
S.M.Kirov Military Medical Academy, Ministry of Defense of the Russian Federation
Email: rakinalex@gmail.com
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