Comparison of the results of molecular biological and bacteriological studies for the identification of methicillin-resistant Staphylococcus strains in bacteremia


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Abstract

Objective. To compare the results of molecular biological and bacteriological studies to identify blood methicillin-resistant staphylococcus strains. Subjects and methods. A parallel bacteriological and molecular biological (real-time PCR) study was conducted to examine biomaterials from 240 patients with signs of infection who were treated in a Moscow multidisciplinary hospital. The biological material was peripheral vein blood taken in vacuum test tubes with EDTA for PCR and in vials containing a nutrient medium for bacteriological testing. Results. The sensitivity and specificity of the PCR assay versus the bacteriological test in detecting blood methicillin-resistant staphylococci were 100 and 97%, respectively. Methicillin-resistant staphylococci were detected by PCR in 6.3% of patients with signs of infection and by bacteriological tests in 3.3% of patients. Conclusion. The real-time PcR assay used for the detection of blood methicillin-resistant staphylococci will be able to reduce the time of a diagnostic study, to increase the possibility of identifying the etiological agent for bacteremia, and to timely perform adequate antimicrobial therapy.

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About the authors

Tatiana S. Skachkova

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: skachkova@inbox.ru
Researcher, Department of Molecular Diagnosis and Epidemiology Moscow, Russia

Natalia N. Lashenkova

N.I. Pirogov National Medical and Surgical Center, Ministry of Health of Russia

Email: lashenkovann@pirogov-center.ru
Bacteriologist, Laboratory of Bacteriology Moscow, Russia

Valeria S. Fomina

N.I. Pirogov National Medical and Surgical Center, Ministry of Health of Russia

Email: fominavs@pirogov-center.ru
Cand. Med. Sci., Chief, Service of Clinical Laboratory Diagnosis Moscow, Russia

Mikhail N. Zamyatin

N.I. Pirogov National Medical and Surgical Center, Ministry of Health of Russia

Email: zamyatinmn@pirogov-center.ru
Professor, MD; Head, Department of Anesthesiology and Resuscitation Moscow, Russia

Vitaly G. Gusarov

N.I. Pirogov National Medical and Surgical Center, Ministry of Health of Russia

Email: gusarov1974@mail.ru
Cand. Med. Sci., Head, Department of Anesthesiology and Resuscitation (Intensive Care) Moscow, Russia

Maria V. Dementienko

N.I. Pirogov National Medical and Surgical Center, Ministry of Health of Russia

Email: dementienkomv@pirogov-center.ru
Anesthesiologist/Resuscitation Specialist Moscow, Russia

Olga Yu. Shipulina

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: shipulina@pcr.ms
Cand. Med. Sci., Head, Laboratory of Molecular Studies, Department of Molecular Diagnosis and Epidemiology Moscow, Russia

Elena N. Goloveshkina

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: goloveshkina@cmd.su
Cand. Biol. Sci., Head, Laboratory of Molecular Diagnosis and Epidemiology of Reproductive Organ Infections Moscow, Russia

Vasily G. Akimkin

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: vgakimkin@yandex.ru
Professor, MD, Academicien of the Russian Academy of Sciences, Director Moscow, Russia

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