Clinical strains of Vibrio cholerae neO1/ neO139 serogroups in Russia: Isolation dynamics, serological belonging, genetic features


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Abstract

Objective. To investigate the detection dynamics and the serological and molecular genetic features of clinical strains of V. cholera popO1/popO139 serogroups isolated in the subjects of the Russian Federation in 2014-2018. Materials and methods. A total of 40 clinical V. cholera nonO1/nonO139 cultures isolated from patients in 6 subjects of the Russian Federation were studied. Bacteriological, serological, and genetic studies were used. Results. The belonging of Vibrio cholerae to 14 serogroups was established; the circulations of specific serogroups in different territories were determined. The strains had different sets of genetic determinants of pathogenicity/ persistence factors. Conclusion. The strains of vibrio cholerae neO1/neO139 serogroups, both isolated from patients in the southern regions of Russia and imported to other subjects from the countries of Southeast Asia and Africa, were characterized by variability in biological properties. Their genetic features are associated, among other things, with the presence of the heat-stable toxin gene (stn) in 4 strains.

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About the authors

Irina V. Arkhangelskaya

Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: irina070769@mail.ru
Cand. Med. Sci., Researcher, Laboratory of Cholera Microbiology Rostov-on-Don, Russia

Vladimir D. Kruglikov

Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: kruglikov_vd@antiplague.ru
MD, Head, Laboratory of Cholera Microbiology Rostov-on-Don, Russia

Daria A. Levchenko

Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: levchenko_da@antiplague.ru
Cand. Med. Sci., Senior Researcher, Laboratory of Cholera Microbiology Rostov-on-Don, Russia

Elena V. Monakhova

Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Biol.D. Head, Group of Molecular Biology Rostov-on-Don, Russia

Natalia B. Nepomnyashchaya

Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Researcher, Group of Molecular Biology Rostov-on-Don, Russia

Oksana A. Podoinitsyna

Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Researcher, Laboratory of Cholera Microbiology Rostov-on-Don, Russia

Maria I. Ezhova

Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Researcher, Laboratory of Cholera Microbiology Rostov-on-Don, Russia

References

  1. Blokesch M., Schoolnik G.K. Serogroup conversion of Vibrio cholerae in aquatic reservoirs. PLoS Pathog. 2007; 3(6): e81. doi: 10.1371/journal.ppat.0030081
  2. Григоренко Л.В., Кругликов В.Д., Мазрухо А.Б., Архангельская И.В., Зубкова Д.А., Непомнящая Н.Б., Шестиалтынова И.С. Холерные вибрионы неО1/неО139, выделенные в ходе мониторинга водоемов и стоков Ростова-на-Дону с 2009 по 2011 год. Пробл. особо опасных инф. 2013; (4): 48-50. https://doi.org/10.21055/0370-1069-2013-4-48-50
  3. Li F., Du P., Li B., Ke C., Chen A., Chen J., Zhou H., Li J., Morris J.G., Kan B., Wang D. Distribution of virulence-associated genes and genetic relationships in non-O1/O139 Vibrio cholerae aquatic isolates from China. Appl. Environ. Microbiol. 2014; 80(16): 4987-92. doi: 10.1128/AEM.01021-14
  4. Marin M.A., Thompson C.C., Freitas F.S., Finseca E.L., Aboderin A.O., Zailani S.B., Quartey N.K., Okeke I.N., Vicente A.C. Cholera outbreaks in Nigeria are associated with multidrug resistant atypical El Tor and non-O1/non-O139 Vibrio cholerae. PLoS Neg l. Trop. Dis. 2013; 7(2): 2049. doi: 10.1371/journal. pntd.0002049
  5. Schirmeister F., Diekmann R., Bechlars S. et al. Genetic and phenotypic analysis of Vibrio cholerae non-O1, non-0139 isolated from German and Austrian patients. Eur. J. Clin. Мicrobiol. Infect. Dis. 2014; (33): 767-78. doi: 10.1007/s10096-013-2011-9
  6. Zmeter C., Tabaya H., Sharara A.I., Kanj S.S. Non-O1, non-0139 Vibrio cholerae septicemia at a tertiary care center in Beirut, Lebanon; a case report and review. J. Infect. Public. Health. 2018; 11(5): 601-4. doi: 10.1016/j.jiph.2018.01.001
  7. Shanley J., Kanj A., El Zein S., Tabaja H., Trzcinski B., Horman J., Salimnia H., Fairfax M., Singh M. Non-O1, non-0139 Vibrio cholerae bacteremia in an urban academic medical center in the United States. IDCases 2019; (5); e00527. https://doi. org/10.1016/j.idcr.2019.e00527
  8. Монахова Е.В., Архангельская И.В. Холерные вибрионы неО1/неО139 серогрупп в этиологии острых кишечных инфекций: современная ситуация в России и в мире. Пробл. особо опасных инф. 2016; (2): 14-23. https://doi. org/10.21055/0370-1069-2016-2-14-23
  9. Chatterjee S., Ghosh K., Raychoudhuri A., Chowdhury G., Bhattacharya M.K., Mukhopadhyay A.K., Ramamurthy T., Bhattacharya S.K., Klose K.E., Nandy R.K. Incidence, virulence factors, and clonality among clinical strains of non-O1, non-0139 Vibrio cholerae isolates from hospitalized diarrheal patients in Kolkata, India. J. Clin. Microbiol. 2009; 47(4): 1087-95. doi: 10.1128/JCM.02026-08
  10. Hasan N.A., Choi S.Y., Eppinger M., Clark P.W., Chen A., Alam M., Haley B.J., Taviani E., Hine E., Su Q., Tallon L.J., Prosper J.B., Furth K., Hoq M.M., Li H., Fraser-Liggett C.M., Cravioto A., Huq A., Ravel J., Cebula T.A., Colwell R.R. Genomic diversity of2010 Haitian cholera outbreak strains. Proc. Natl. Acad. Sci. USA 2012; 109(29): 2010-17. doi: 10.1073/pnas.120735910.
  11. Селянская Н.А., Архангельская И.В., Водопьянов А.С., Водопьянов С.О., Кругликов В.Д., Водяницкая С.Ю., Веркина Л.М., Непомнящая Н.Б. Типирование штаммов Vibrio cholerae неО1/неО139, изолированных в Ростовской области в 2014 году. Журнал микробиологии, эпидемиологии и иммунобиологии 2016; (1): 3-9. https://doi. org/10.36233/0372-9311-2016-1-3-9
  12. Телесманич Н.Р., Чайка С.О., Чайка И.А., Гончаренко Е.В., Ломов Ю.М. Масс-спектрометрический анализ MALDI-TOF в идентификации и типировании штаммов холерных вибрионов. Клин. лаб. диагностика 2016; 61(6): 375-9. doi: 10.18821/0869-2084-2016-6-375-379
  13. Монахова Е.В., Архангельская И.В., Писанов Р.В., Титова С.В. Особенности первичной структуры цитотоксина MARTX нетоксигенных штаммов Vibrio cholerae разных серогрупп. Пробл. особо опасных инф. 2018; (3): 73-7. https://doi.org/10.21055/0370-1069-2018-3-73-77
  14. Монахова Е.В., Архангельская И.В., Титова С.В., Писанов Р.В. MSHA-подобные пили нетоксигенных штаммов холерных вибрионов. Пробл. особо опасных инф. 2019; (3): 75-80
  15. Caldini G., Neri A., Cresti S., Boddi V., Rossolini G.M., Lanciotti E. High prevalence of Vibrio cholerae non-O1 carrying heat-stable-enterotoxin-encoding genes among Vibrio isolates from a temperate-climate river basin of central Italy. Appl. Environ. Microbiol. 1997; 63(7): 2934-9.
  16. Octavia S., Salim A., Kurniawan J., Lam C., Leung Q., Ahsan S., Reeves P.R., N air G.B., Lan R. Population structure and evolution of non-o1/non-O139 Vibrio cholerae by multilocus sequence typing. PLoS One 2013; 8(6): 65342. doi: 10.1371/ journal.pone.0065342
  17. Ceccarelli D., Chen A., Hasan N.A., Rashed S.M., Huq A., Colwell R.R. Non-O1/non-O139 Vibrio cholerae carrying multiple virulence factors and V.cholerae O1 in the Chesapeake Bay, Maryland. Appl. Environ Microbiol. 2015; 81: 1909-18. doi: 10.1128/AEM.03540-14.

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