Phenotypic and molecular genetic analysis of Brucella spp. strains isolated from atypical carriers
- 作者: Serdyuk N.S.1, Zharinova N.V.1, Zhilchenko E.B.1, Karapetyan M.G.1, Belozerova O.N.1
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隶属关系:
- Stavropol Anti-Plague Institute
- 期: 卷 15, 编号 2 (2025)
- 页面: 80-84
- 栏目: Original Investigations
- URL: https://journals.eco-vector.com/2226-6976/article/view/686737
- DOI: https://doi.org/10.18565/epidem.2025.15.2.80-84
- ID: 686737
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详细
Objective. Evaluation of the phenotypic and genetic properties of Brucella spp. isolated in the Russian Federation from different types of carriers.
Materials and methods. We used 38 strains of the causative agent of brucellosis from the collection of the Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being. The phenotypic properties of the cultures were studied using generally accepted phenotypic methods. Whole genome sequencing of DNA of 38 isolates was performed using Ion PGM (Life Technologies, USA).
Results. The cultures had typical tinctorial, morphological and cultural properties characteristic of B. melitensis, B. abortus, B. suis. As a result of the comprehensive study, it was established that the studied strains of the causative agent of brucellosis (B. melitensis, B. abortus and B. suis), isolated in the territory of the Russian Federation from atypical hosts, do not differ in their cultural-morphological, molecular-genetic properties from the cultures isolated from the main carriers of the infection.
Conclusion. Brucella strains retain their basic properties when passing from the main hosts to atypical carriers.
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作者简介
Natalia Serdyuk
Stavropol Anti-Plague Institute
Email: nata.serduk.83@mail.ru
ORCID iD: 0000-0001-5350-0111
Сand. Biol. Sci., Senior Researcher at the Collection of Pathogenic Microorganisms Laboratory
俄罗斯联邦, StavropolNina Zharinova
Stavropol Anti-Plague Institute
Email: stavnipchi@mail.ru
ORCID iD: 0000-0001-6718-4877
Сand. Biol. Sci., Senior Researcher at the Collection of Pathogenic Microorganisms Laboratory
俄罗斯联邦, StavropolElena Zhilchenko
Stavropol Anti-Plague Institute
Email: stavnipchi@mail.ru
ORCID iD: 0000-0001-7180-1292
Сand. Biol. Sci., Head of the Collection of Pathogenic Microorganisms Laboratory
俄罗斯联邦, StavropolManya Karapetyan
Stavropol Anti-Plague Institute
Email: stavnipchi@mail.ru
ORCID iD: 0009-0005-3006-5504
Junior Researcher at the Collection of Pathogenic Microorganisms Laboratory
俄罗斯联邦, StavropolOlga Belozerova
Stavropol Anti-Plague Institute
编辑信件的主要联系方式.
Email: stavnipchi@mail.ru
ORCID iD: 0009-0002-5570-557X
Junior Researcher at the Collection of Pathogenic Microorganisms Laboratory
俄罗斯联邦, Stavropol参考
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