Phenotypic and molecular genetic analysis of Brucella spp. strains isolated from atypical carriers

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Objective. Evaluation of the phenotypic and genetic properties of Brucella spp. isolated in the Russian Federation from different types of carriers.

Materials and methods. We used 38 strains of the causative agent of brucellosis from the collection of the Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being. The phenotypic properties of the cultures were studied using generally accepted phenotypic methods. Whole genome sequencing of DNA of 38 isolates was performed using Ion PGM (Life Technologies, USA).

Results. The cultures had typical tinctorial, morphological and cultural properties characteristic of B. melitensis, B. abortus, B. suis. As a result of the comprehensive study, it was established that the studied strains of the causative agent of brucellosis (B. melitensis, B. abortus and B. suis), isolated in the territory of the Russian Federation from atypical hosts, do not differ in their cultural-morphological, molecular-genetic properties from the cultures isolated from the main carriers of the infection.

Conclusion. Brucella strains retain their basic properties when passing from the main hosts to atypical carriers.

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作者简介

Natalia Serdyuk

Stavropol Anti-Plague Institute

Email: nata.serduk.83@mail.ru
ORCID iD: 0000-0001-5350-0111

Сand. Biol. Sci., Senior Researcher at the Collection of Pathogenic Microorganisms Laboratory

俄罗斯联邦, Stavropol

Nina Zharinova

Stavropol Anti-Plague Institute

Email: stavnipchi@mail.ru
ORCID iD: 0000-0001-6718-4877

Сand. Biol. Sci., Senior Researcher at the Collection of Pathogenic Microorganisms Laboratory

俄罗斯联邦, Stavropol

Elena Zhilchenko

Stavropol Anti-Plague Institute

Email: stavnipchi@mail.ru
ORCID iD: 0000-0001-7180-1292

Сand. Biol. Sci., Head of the Collection of Pathogenic Microorganisms Laboratory

俄罗斯联邦, Stavropol

Manya Karapetyan

Stavropol Anti-Plague Institute

Email: stavnipchi@mail.ru
ORCID iD: 0009-0005-3006-5504

Junior Researcher at the Collection of Pathogenic Microorganisms Laboratory

俄罗斯联邦, Stavropol

Olga Belozerova

Stavropol Anti-Plague Institute

编辑信件的主要联系方式.
Email: stavnipchi@mail.ru
ORCID iD: 0009-0002-5570-557X

Junior Researcher at the Collection of Pathogenic Microorganisms Laboratory

俄罗斯联邦, Stavropol

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2. Figure. Dendrogram of strains of different Brucella types

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