New opportunities to diagnose HHV-8 infection by enzyme immunoassay


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Abstract

Objective. To create a reagent kit for detecting specific IgG antibodies to human herpesvirus type 8 (HHV- 8) by solid-phase enzyme-linked immunosorbent assay (ELISA). Subjects and methods. ELISA was used to examine serum samples from HIV-infected, healthy donors, children, and elderly people for specific anti-HHV-8 antibodies, by confirming the results by immunofluorescence assay (IFA). The activity and cross-reactivity of a new reagent kit for detection of other herpesviruses were investigated. Results. The created reagent set for the detection of specific IgG antibodies to HHV8 by ELISA corresponds to the declared values. Conclusion. An ELISA-based diagnostic test will be able to rapidly screen a large number of sera for the identification of people at risk for Kaposi’s sarcoma and to conduct seroepidemiological studies.

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About the authors

Elena A. Amelina

ECOlab

Email: ekolab-ferment@mail.ru
Cand. Biol. Sci., Head, Department of Advanced Developments Elektrogorsk, Moscow Region, Russia

Veronika A. Arsenyeva

ECOlab

Email: ecolab.arsenyeva@gmail.com
Elektrogorsk, Moscow Region, Russia

Sarkhan S. Mardanly

ECOlab; Honorary Academician N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia

Junior Researcher, Department of Epidemiology, Honorary Academician N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russia; Deputy General Director for Commercial Affairs, EKOlab Elektrogorsk, Moscow Region, Russia

Seyfaddin G. Mardanly

ECOlab; State University for Humanities and Technology

Email: ekolab-president@mail.ru
MD, Academician of the Russian Academy of Medical and Technical Sciences, Honored Health Care Worker of the Russian Federation; Professor, Department of Pharmacology and Pharmaceutical Sciences, Moscow State University for Humanities and Technology; President and Director of Science, ECOlab Elektrogorsk, Moscow Region, Russia

Sergey V. Rotanov

Moscow Research and Practical Center of Dermatovenereology and Cosmetology, Moscow Healthcare Department

Email: svrotanov@mail.ru
MD, Associate Professor, Leading Researcher Moscow, Russia

References

  1. Марданлы С.Г. Герпесвирусные инфекции. Учебное пособие. Орехово-Зуево: ГГТУ, 2017. 144 с. Mardanly S.G. [The herpes virus infections. Textbook]. Orekhovo-Zuevo: Gosudarstvennyy gumanitarno-tekhnologicheskiy universitet, 2017. 144 р. (In Russ.).
  2. Потапова Л.А., Косякова Н.П., Махнева Н.В., Мезенцева М.В. Вирус герпеса 8-го типа и его роль в патологии человека. Вопросы вирусологии 2009; (6): 18-23. Potapova L.A., Kosyakova N.P., Mahneva N.V., Mezenceva M.V. [Herpes virus type 8 and its role in human pathology]. Voprosy virusologii 2009; (6): 18-23. (In Russ.).
  3. Lennette E.T., Blackbourn D.J., Levy J.A. Antibodies to Human herpesvirus type 8 in the general population and in Kaposi’s sarcoma patients. Lancet 1996; 348: 858-61.
  4. Ablashi D.V., Chatlynne L.G., Whitman J.E. Jr., Cesarman E. Spectrum of Kaposi’s Sarcoma-Associated Herpesvirus, or Human Herpesvirus 8 Diseases. Clin. Microbiol. Rev. 2002; 15(3): 439-64.
  5. Fukumoto H., Kanno T., Hasegawa H., Katano H. Pathology of Kaposi’s sarcoma-associated herpesvirus infection. Front. Microbiol. 2011; (2): 175.
  6. Dupin N., Grandadam M., Calvez V., Gorin I., Aubin J.T., Havard S. et al. Herpesviruslike DNA sequences in patients with Mediterranean Kaposi’s sarcoma. Lancet 1995; 345: 761-62.
  7. Chang Y., Cesarman E., Pessin M. S., Lee F., Culpepper J., Knowles D.M., Moore P.S. Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi’s sarcoma. Science 1994; 266: 1865-69.
  8. Huang Y.Q., Li J.J., Kaplan M.H., Poiesz B., Katabira E., Zhang W.C. et al. Human herpesvirus-like nucleic acid in various forms of Kaposi’s sarcoma. Lancet 1995; 345: 759-61.
  9. Moore P.S., Chang Y. Detection of herpesvirus-like DNA sequences in Kaposi’s sarcoma in patients with and without HIV infection. N. Engl. J. Med. 1995; 332: 1181-85.
  10. Moore P.S., Kingsley L.A., Holmberg S.D., Spira T., Gupta P., Hoover D.R. et al. Kaposi’s sarcoma-associated herpesvirus infection prior to onset of Kaposi’s sarcoma. AIDS 1996; (10): 175-80.
  11. Gao S.J., Kingsley L., Li M., Zheng W., Parravicini C., Ziegler J. et al. KSHV antibodies among Americans, Italians and Ugandans with and without Kaposi’s sarcoma. Nat. Med. 1996; (2): 925-28.
  12. Kedes D.H., Ganem D., Ameli N., Bacchetti P., Greenblatt R. The prevalence of serum antibody to human herpesvirus 8 (Kaposi sarcomaassociated herpesvirus) among HIV-seropositive and high-risk HIV-seronegative women. JAMA 1997; 277: 478-81.
  13. Kedes D.H., Operskalski E., Busch M., Kohn R., Flood J., Ganem D. The seroepidemiology of human herpesvirus 8 (Kaposi’s sarcomaassociated herpesvirus): distribution of infection in KS risk groups and evidence for sexual transmission. Nat. Med. 1996; 2: 918-24.
  14. Koelle D.M., Huang M.L., Chandran B., Vieira J., Piepkorn M., Corey L. Frequent detection of Kaposi’s sarcoma-associated herpesvirus Huuman herpes virus 8) DNA in saliva of human immunodeficiency virus infected men: clinical and immunologic correlates. Infect. Dis. 1997; 176: 94-102.
  15. Simpson G.R., Schulz T.F., Whitby D., Cook P.M., Boshoff C., Rainbow L. et al. Prevalence of Kaposi’s sarcoma associated herpesvirus infection measured by antibodies to recombinant capsid protein and latent immunofluorescence antigen. Lancet 1996; 348: 1133-38.
  16. Smith M.S., Bloomer C., Horvat R., Goldstein E., Casparian J.M., Chandran B. Detection of human herpesvirus 8 DNA in Kaposi’s sarcoma lesions and peripheral blood of human immunodeficiency virus-positive patients and correlation with serologic measurements. J. Infect. Dis. 1997; 176: 84-93.
  17. Spira T.J., Lan L., Dollard S.C., Meng Y.H. et al. Comparison of serologic assays and PCR for diagnosis of Human herpesvirus 8 infection. J. Clin. Microbiol. 2000; 38(6): 2174-80.
  18. Yang T.-C., Chang C.-P., Lan Y.-C., Liu C.-L., Shih M.-C., Wu F.-Y., Lin C.-W. Recombinant ORF66 and ORFK12 antigens for the detection of Human herpesvirus 8 antibodies in HIV-positive and -negative patients. Biotechnology Letters 2009; 31(5): 629-37.
  19. Katano H., Iwasaki T., Baba N., Terai M., Mori S., Iwamoto A., Kurata T., Sata T. Identification of Antigenic Proteins Encoded by Human Herpesvirus 8 and Seroprevalence in the General Population and among Patients with and without Kaposi’s Sarcoma. J. Virol. 2000; 74(8): 3478-85.
  20. Tedeschi R., Dillner J., De Paoli P. Laboratory diagnosis of Human herpesvirus 8 infection in humans. Eur. J. Clin. Microbiol. Infect. Dis. 2002; 21: 831-44.
  21. Moses A.V., Fish K.N., Ruhl R., Smith P.P., Strussenberg J.G., Zhu L. et al. Long-term infection and transformation of dermal microvascular endothelial cells by Human herpesvirus 8. J. Virol 1999; 73 (8): 892-902.
  22. Zhu F.X., Chong J.M., Wu L., Yuan Y. Virion proteins of Kaposi’s sarcoma-associated herpesvirus. J. Virol. 2005; 79(2): 800-11.
  23. Katano H., Iwasaki T., Baba N., Teral M., Iwamoto S.M., Kurata T., Sata T. Identification of antigenic proteins encoded by human herpesvirus 8 and seroprevalence in general population among patients with and without Kaposi’s sarcoma. Virology 2000; 74 (8): 3478-85.

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