New opportunities to diagnose HHV-8 infection by enzyme immunoassay


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Objective. To create a reagent kit for detecting specific IgG antibodies to human herpesvirus type 8 (HHV- 8) by solid-phase enzyme-linked immunosorbent assay (ELISA). Subjects and methods. ELISA was used to examine serum samples from HIV-infected, healthy donors, children, and elderly people for specific anti-HHV-8 antibodies, by confirming the results by immunofluorescence assay (IFA). The activity and cross-reactivity of a new reagent kit for detection of other herpesviruses were investigated. Results. The created reagent set for the detection of specific IgG antibodies to HHV8 by ELISA corresponds to the declared values. Conclusion. An ELISA-based diagnostic test will be able to rapidly screen a large number of sera for the identification of people at risk for Kaposi’s sarcoma and to conduct seroepidemiological studies.

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作者简介

Elena Amelina

ECOlab

Email: ekolab-ferment@mail.ru
Cand. Biol. Sci., Head, Department of Advanced Developments Elektrogorsk, Moscow Region, Russia

Veronika Arsenyeva

ECOlab

Email: ecolab.arsenyeva@gmail.com
Elektrogorsk, Moscow Region, Russia

Sarkhan Mardanly

ECOlab; Honorary Academician N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia

Junior Researcher, Department of Epidemiology, Honorary Academician N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russia; Deputy General Director for Commercial Affairs, EKOlab Elektrogorsk, Moscow Region, Russia

Seyfaddin Mardanly

ECOlab; State University for Humanities and Technology

Email: ekolab-president@mail.ru
MD, Academician of the Russian Academy of Medical and Technical Sciences, Honored Health Care Worker of the Russian Federation; Professor, Department of Pharmacology and Pharmaceutical Sciences, Moscow State University for Humanities and Technology; President and Director of Science, ECOlab Elektrogorsk, Moscow Region, Russia

Sergey Rotanov

Moscow Research and Practical Center of Dermatovenereology and Cosmetology, Moscow Healthcare Department

Email: svrotanov@mail.ru
MD, Associate Professor, Leading Researcher Moscow, Russia

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