ANALYSIS AND SPREAD OF GYRA GENE MUTATIONS ENCODING DRUG RESISTANCE TO FLUOROQUINOLONES IN M. TUBERCULOSIS
- Авторлар: SALINA T.Y.1, MOROZOVA T.I.1,2
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Мекемелер:
- Razumovsky Saratov State Medical University, Ministry of Health of Russia
- Saratov Regional Clinical Tuberculosis Dispensary
- Шығарылым: № 6 (2013)
- Беттер: 72-74
- Бөлім: Articles
- URL: https://journals.eco-vector.com/2226-6976/article/view/277272
- ID: 277272
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Аннотация
To investigate the spectrum and spread of gyrA gene mutations, 69 sputum samples from patients with active pulmonary tuberculosis were examined using biological microchips (BIOCHIP-IMB, Moscow). M. tuberculosis DNA was isolated in 60 (86.9%) people. gyrA gene mutations were found in 12 (20%) patients; the mutations were established in codons 88, 90, 91, and 94. Six (50%) showed a combination of two or more mutations. Twenty-nine (48.3%) persons had a codon 95 mutation that was caused by natural gene polymorphism and failed to result in resistance. Thus, the patients living in the Saratov Region are recorded to have a high level of gyrA gene mutations encoding drug resistance to fluoroquinolones.
Негізгі сөздер
Толық мәтін
Авторлар туралы
Tatyana SALINA
Razumovsky Saratov State Medical University, Ministry of Health of Russia
Email: SalinaTU@rambler.ru
T. MOROZOVA
Razumovsky Saratov State Medical University, Ministry of Health of Russia; Saratov Regional Clinical Tuberculosis Dispensary
Email: dispans@san.ru
Әдебиет тізімі
- Борисов С.Е., Мохирева Л.В., Иванушкина Т.И., Литвинова Н.В. Фторхинолоны в лечении туберкулеза - в поисках новой парадигмы (обзор зарубежной литературы). Туберкулез и болезни легких 2013; 11: 5-16.
- Hooper D.C., Wolfson J.S. The fluoroquinolines: pharmacology, clinical uses, and toxicities in humans. Antimicrob. Agents Chemother. 1985; 28: 716-721.
- Tsukamura M. In vitro antituberculosis activity of a new antibacterial substance ofloxacin (DL8280). Am. Rev. Respir. Dis. 1985; 131: 348-351.
- Truffot-Pernot C., Ji B., Grosset J. Activities of pefloxacin and ofloxacin against mycobacteria in vitro and mouse experiments. Tubercle 1991; 72: 57-64.
- Morozova T.I., Salina T.Yu. Molecular characterization of strains of M. tuberculosis, are resistant to fluoroquinolones, which circulate in the territory of the Saratov region. Eur. Respir. J. 2012; 40(Suppl. 56): 2646
- Takiff N.E. Cloning and nucleotide seguence of M. tuberculosis gyrA and gyrB genes and detection of quinolone resistance mutations. Antimicrob. Agents Chemother. 1994; 38: 773-780.
- Титов Л.П., Сетаре М., Суркова Л.К. Характеристика лекарственной устойчивости M. tuberculosis, выделенных от больных туберкулезом и молекулярные характеристики мутаций в гене gyrA. Медицинские новости 2009; 12: 64-66.
- Степашин Ю.Г., Степашина В.Н., Шемякин И.Г. Молекулярные механизмы устойчивости Mycobacterium tuberculosis к лекарственным препапратам. Антибиотики и химиопрепараты 1999; 44(4): 39-43.
- Tokuea Y., Sugano K., Nodaa T. et al. DNA gyrase gyrA gene mutations in ofloxacin-resistant Staphylococcus aureus. International Journal of Аntimicrobial Аgents 1995; 6: 123126.
- Грядунов Д.А., Лапа С.А., Соболев А.Ю. и др. Разработка диагностических систем на основе биологических микрочипов для идентификации и определения чувствительности возбудителя туберкулеза - M. tuberculosis. Научные труды к 75-летию ведущего противотуберкулёзного учреждения г. Москвы. М., 2001: 233-236.
- Сетаре М. Молекулярно-генетическая характеристика лекарственно-резистентных штаммов M. tuberculosis, выделенных от больных туберкулезом. Автореф. дис.. канд. биол. наук. Минск, 2009.
- Zhao B.Y., Pine R., Domagala J., Drlika K. Fluoroguinolone action againts clinical isolates of Mycobacterium tuberculosis: effects of a C-8 metoxyl group on survival in liguid media and in human macrophages. Antimicrob. Agents Chemother. 1999; 43: 661-666.
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