Development of a Dot-assay for detecting antibodies to tick-borne encephalitis virus using recombinant EDIII-TBEV antigen and IgY antibodies
- Авторлар: Poltavchenko A.G.1, Filatov P.V.1, Ersh A.V.1, Ushkalenko N.D.1, Shanshin D.V.1, Kolosova E.A.1, Shcherbakov D.N.1
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Мекемелер:
- State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
- Шығарылым: Том 14, № 2 (2024)
- Беттер: 63-69
- Бөлім: Original Investigations
- URL: https://journals.eco-vector.com/2226-6976/article/view/635135
- DOI: https://doi.org/10.18565/epidem.2024.14.2.63–9
- ID: 635135
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Аннотация
Objective. Evaluation of the effectiveness of the use of recombinant protein EDIII-TBEV and chicken IgY antibodies in the development of a diagnostic kit for detecting IgG to the tick-borne encephalitis virus by dot analysis.
Materials and methods. Probes based on colloidal gold and secondary immunoreagents (Au-SpA, Au-SpG, Au-IgG-a/Hum and Au-IgY-a/Hum) were manufactured and studied in a dot analysis of human immunoglobulins of A, M and G classes. Experimental kits for dot-immunoassay of IgG to TBEV were created based on the probes and EDIII-TBEV.
Results. Experimental assessment of the characteristics of the kits using panels of blood serum samples containing and not containing IgG to TBEV, as well as panels of samples containing antibodies to pathogens of other flavivirus infections (dengue fever and yellow fever) showed 100% sensitivity and specificity of the dot analysis, which indicates the high selectivity of the recombinant EDIII-TBEV antigen. Probes based on polyclonal IgY-a/Hum are not inferior in sensitivity and provide a 10-20% more intense optical signal compared to probes based on mouse monoclonal IgG-a/Hum.
Conclusion. Probes based on IgY-a/Hum and recombinant EDIII-TBEV antigen can be considered as promising reagents for antibody analysis, in particular, for dot analysis on flat substrates. Kits created on their basis can be used to diagnose tick-borne encephalitis in remote areas with poorly developed laboratory infrastructure.
Негізгі сөздер
Толық мәтін
Авторлар туралы
Alexander Poltavchenko
State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Хат алмасуға жауапты Автор.
Email: poltav@vector.nsc.ru
ORCID iD: 0000-0003-2408-5611
ВD, Leading Researcher
Ресей, Koltsovo, Novosibirsk RegionPavel Filatov
State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Email: filatov_pv@vector.nsc.ru
ORCID iD: 0000-0001-7763-3808
Cand. Biol. Sci., Researcher
Ресей, Koltsovo, Novosibirsk RegionAnna Ersh
State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Email: ersh_av@vector.nsc.ru
ORCID iD: 0000-0002-9220-1250
Cand. Biol. Sci., Researcher
Ресей, Koltsovo, Novosibirsk RegionNikita Ushkalenko
State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Email: ushkalenko_nd@vector.nsc.ru
ORCID iD: 0000-0002-2171-7444
Postgraduate Student, Junior Researcher
Ресей, Koltsovo, Novosibirsk RegionDaniil Shanshin
State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Email: shanshin_dv@vector.nsc.ru
ORCID iD: 0000-0001-9985-1582
Junior Researcher
Ресей, Koltsovo, Novosibirsk RegionEvgeniуa Kolosova
State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Email: kurchanovaea@gmail.com
ORCID iD: 0000-0002-8967-4719
Junior Researcher
Ресей, Koltsovo, Novosibirsk RegionDmitry Shcherbakov
State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Email: scherbakov_dn@vector.nsc.ru
ORCID iD: 0000-0001-8023-4453
Cand. Biol. Sci., Leading Researcher
Ресей, Koltsovo, Novosibirsk RegionӘдебиет тізімі
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