Development of a Dot-assay for detecting antibodies to tick-borne encephalitis virus using recombinant EDIII-TBEV antigen and IgY antibodies

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Objective. Evaluation of the effectiveness of the use of recombinant protein EDIII-TBEV and chicken IgY antibodies in the development of a diagnostic kit for detecting IgG to the tick-borne encephalitis virus by dot analysis.

Materials and methods. Probes based on colloidal gold and secondary immunoreagents (Au-SpA, Au-SpG, Au-IgG-a/Hum and Au-IgY-a/Hum) were manufactured and studied in a dot analysis of human immunoglobulins of A, M and G classes. Experimental kits for dot-immunoassay of IgG to TBEV were created based on the probes and EDIII-TBEV.

Results. Experimental assessment of the characteristics of the kits using panels of blood serum samples containing and not containing IgG to TBEV, as well as panels of samples containing antibodies to pathogens of other flavivirus infections (dengue fever and yellow fever) showed 100% sensitivity and specificity of the dot analysis, which indicates the high selectivity of the recombinant EDIII-TBEV antigen. Probes based on polyclonal IgY-a/Hum are not inferior in sensitivity and provide a 10-20% more intense optical signal compared to probes based on mouse monoclonal IgG-a/Hum.

Conclusion. Probes based on IgY-a/Hum and recombinant EDIII-TBEV antigen can be considered as promising reagents for antibody analysis, in particular, for dot analysis on flat substrates. Kits created on their basis can be used to diagnose tick-borne encephalitis in remote areas with poorly developed laboratory infrastructure.

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作者简介

Alexander Poltavchenko

State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

编辑信件的主要联系方式.
Email: poltav@vector.nsc.ru
ORCID iD: 0000-0003-2408-5611

ВD, Leading Researcher

俄罗斯联邦, Koltsovo, Novosibirsk Region

Pavel Filatov

State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: filatov_pv@vector.nsc.ru
ORCID iD: 0000-0001-7763-3808

Cand. Biol. Sci., Researcher

俄罗斯联邦, Koltsovo, Novosibirsk Region

Anna Ersh

State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: ersh_av@vector.nsc.ru
ORCID iD: 0000-0002-9220-1250

Cand. Biol. Sci., Researcher

俄罗斯联邦, Koltsovo, Novosibirsk Region

Nikita Ushkalenko

State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: ushkalenko_nd@vector.nsc.ru
ORCID iD: 0000-0002-2171-7444

Postgraduate Student, Junior Researcher

俄罗斯联邦, Koltsovo, Novosibirsk Region

Daniil Shanshin

State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: shanshin_dv@vector.nsc.ru
ORCID iD: 0000-0001-9985-1582

Junior Researcher

俄罗斯联邦, Koltsovo, Novosibirsk Region

Evgeniуa Kolosova

State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: kurchanovaea@gmail.com
ORCID iD: 0000-0002-8967-4719

Junior Researcher

俄罗斯联邦, Koltsovo, Novosibirsk Region

Dmitry Shcherbakov

State Scientific Center for Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: scherbakov_dn@vector.nsc.ru
ORCID iD: 0000-0001-8023-4453

Cand. Biol. Sci., Leading Researcher

俄罗斯联邦, Koltsovo, Novosibirsk Region

参考

  1. О состоянии санитарно-эпидемиологического благополучия населения в Российской Федерации в 2022 году: Государственный доклад. М.: Федеральная служба по надзору в сфере защиты прав потребителей и благополучия человека, 2023, 340 с. https://www.rospotrebnadzor.ru/documents/details.php?ELEMENT_ID=25076&ysclid=lw6gk5dg81393380238 [On the state of sanitary and epidemiological welfare of the population in the Russian Federation in 2022: State Report]. Moscow, 2023. 340 p. (In Russ.). https://www.rospotrebnadzor.ru/documents/details.php?ELEMENT_ID=25076&ysclid=lw6gk5dg81393380238
  2. Колясникова Н.М., Ишмухаметов А.А., Акимкин В.Г. Современное состояние проблемы клещевого энцефалита в России и мире. Эпидемиология и Вакцинопрофилактика 2023; 22(1): 104–23. doi: 10.31631/2073-3046-2023-22-1-104-123 Kolyasnikova N.M., Ishmukhametov A.A., Akimkin V.G. [The current state of the problem of tick-borne encephalitis in Russia and the world]. Epidemiology and Vaccinal Prevention 2023; 22(1): 104–23. (In Russ.). doi: 10.31631/2073-3046-2023-22-1-104-123
  3. Darwish N.T., Alias Y.B., Khor S.M. An introduction to dengue-disease diagnostics. TrAC Trends Analyt. Chem. 2015; 67: 45–55. doi: 10.1016/j.trac.2015.01.005
  4. Cleton N.B., Godeke G.J., Reimerink J., Beersma M.F., Franco L. Goeijenbier M.et al. Spot the Difference-Development of a Syndrome Based Protein Microarray for Specific Serological Detection of Multiple Flavivirus Infections in Travelers. PLOS Neglected Tropical Diseases 2015; 9(3): e0003580. doi: 10.1371/journal.pntd.0003580
  5. Полтавченко А.Г., Ерш А.В., Филатов П.В., Ушкаленко Н.Д. Мультиплексный дот-иммуноанализ в диагностике инфекционных заболеваний: Монография. Чебоксары: ООО «Издательский дом «Среда», 2022. 224 с. doi: 10.31483/a-10421 Poltavchenko A.G., Ersh A.V., Filatov P.V., Ushkalenko N.D. [Multiplex dot-immunoassay in the diagnosis of infectious diseases: Monograph]. Cheboksary: LLC «Publishing House «Sreda», 2022. 224 p. (In Russ.). doi: 10.31483/a-10421
  6. Полтавченко А.Г., Ерш А.В., Азаев М.Ш., Филатов П.В. Оптимизация условий проявления результатов дот-иммуноанализа в автономном наборе для выявления ортопоксвирусов. Эпидемиол. и инфекц. бол. 2019; 24(2): 77–83. doi: 10.18821/1560-9529-2019-24-2-77-83 Poltavchenko A.G., Ersh A.V., Azaev M.S., Filatov P.V. [Optimization of the conditions for the development of the results of the dot-immunoassay in an autonomous kit for the detection of orthopoxviruses]. Epidemiology and Infectious Diseases 2019; 24(2): 77–83. (In Russ.). doi: 10.18821/1560-9529-2019-24-2-77-83
  7. Poltavchenko A.G., Zaitsev B.N., Ersh A.V., Korneev D.V., Tarasov O.S., Filatov P.V. et al. The selection and optimization of the detection system for self-contained multiplexed dot-immunoassay. J. Immunoassay Immunochem. 2016; 37(5): 540–54. doi: 10.1080/15321819.2016.1174134
  8. Wu R., Yakhkeshi S., Zhang X. Scientometric analysis and perspective of IgY technology study. Poultry Science 2022; 101(4): 101713. doi: 10.1016/j.psj.2022.101713
  9. Yakhkeshi S., Wu R., Chelliappan B., Zhang X. Trends in industrialization and commercialization of IgY technology. Front. Immunol. 2022; 13: 991931. doi: 10.3389/fimmu.2022.991931
  10. Иванов А.П., Клеблеева Т.Д., Иванова О.Е. Опыт применения IgY-технологии для лабораторной диагностики вирусных инфекций. Вопросы вирусологии 2020; 65(1): 21–6. doi: 10.36233/0507-4088-2020-65-1-21-26 Ivanov A.P., Klebleeva T.D., Ivanova O.E. [Experience of application of IgY-technology for laboratory diagnostics of viral infections]. Problems of Virology 2020; 65(1): 21–6. (In Russ.). doi: 10.36233/0507-4088-2020-65-1-21-26
  11. Anthony B.F., Concepcion I.E., Concepcion N.F., Vadheim C.M., Tiwari J. Relation between maternal age and serum concentration of IgG antibody to type III group B streptococci. J. Infect. Dis. 1994; 170(3): 717–20. doi: 10.1093/infdis/170.3.717
  12. Granoff D.M., Shackelford P.G., Suarez B.K., Nahm M.H., Cates K.L., Murphy T.V. et al. Hemophilus influenzae type B disease in children vaccinated with type B polysaccharide vaccine. N. Engl. J. Med. 1986; 315(25): 1584–90. doi: 10.1056/NEJM198612183152505
  13. Bloom J.W., Wong M.F., Mitra G. Detection and reduction of protein A contamination in immobilized protein A purified monoclonal antibody preparations. J. Immunol. Methods 1989; 117(1): 83–9. doi: 10.1016/0022-1759(89)90121-x
  14. Gaspar-Castillo C., Rodríguez M.H., Ortiz-Navarrete V., Alpuche-Aranda C.M and Martinez-Barnetche J. Structural and immunological basis of cross-reactivity between dengue and Zika infections: Implications in serosurveillance in endemic regions. Front. Microbiol. 2023; 14: 1107496. doi: 10.3389/fmicb.2023.1107496
  15. Girl P., Bestehorn-Willmann M., Zange S., Borde J.P., Dobler G. Tick-borne encephalitis virus nonstructural protein 1 IgG enzyme-linked immunosorbent assay for differentiating infection versus vaccination antibody responses. J. Clin. Microbiol. 2020; 58(4): e01783-19. doi: 10.1128/jcm.01783-19
  16. Inagaki E., Sakai M., Hirano M., Muto M., Kobayashi S., Kariwa H. et al. Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles. Ticks Tick Borne Dis. 2016; 7(5): 723–9. doi: 10.1016/j.ttbdis.2016.03.002
  17. Pulkkinen L.I.A., Barrass S.V., Domanska A., Överby A.K., Anastasina M., Butcher S.J. Molecular Organisation of Tick-Borne Encephalitis Virus. Viruses 2022; 14(4): 792. doi: 10.3390/v14040792
  18. Барышникова В.С., Турченко Ю.В., Шишова А.А., Климентов А.С., Тучинская К.К., Карганова Г.Г. Рекомбинантный гликопротеин E вируса клещевого энцефалита для создания дифференцирующей тест-системы. Биотехнология 2022; 38(6): 73–83. doi: 10.56304/S0234275822060023 Baryshnikova V.S., Turchenko Yu.V., Shishova A.A., Klimentov A.S., Tuchynskaya K.K., Karganova G.G. [Recombinant Glycoprotein E of Tick-Borne Encephalitis Virus for a Developing Differentiated Test System]. Biotechnology 2022; 38(6): 73–83. (In Russ.). doi: 10.56304/S0234275822060023
  19. Holbrook M.R., Shope R.E., Barrett A.D.T. Use of Recombinant E Protein Domain III-Based Enzyme-Linked Immunosorbent Assays for Differentiation of Tick-Borne Encephalitis Serocomplex Flaviviruses from Mosquito-Borne Flaviviruses. J. Clin. Microbiol. 2004; 42(9): 4101–10. doi: 10.1128/JCM.42.9.4101-4110.2004
  20. Ludolfs D., Reinholz M., Schmitz H. Highly specific detection of antibodies to tick-borne encephalitis (TBE) virus in humans using a domain III antigen and a sensitive immune complex (IC) ELISA. J. Clin. Virol. 2009; 45(2): 125‒8. doi: 10.1016/j.jcv.2009.03.016

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2. Figure. Comparative evaluation of the sensitivity of detecting human immunoglobulins (analytes)in a dot immunoassay using colloidal gold associated with different detection reagents

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