FEATURES OF CIRCULATION B. PERTUSSIS STRAINS ISOLATED FROM WHOOPING COUGH PATIENTS WITH DIFFERENT ALLELIC VARIANTS OF THE GENE ENCODING THE PERTUSSIS TOXIN PROMOTER (ptxP) REGION


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The structural features of the regulatory ptxP gene were analyzed in the B. pertussis strains currently isolated from whooping cough patients in Russia. The materials of the investigation were 117 B. pertussis strains isolated from whooping cough patients in Moscow, the Moscow, Novosibirsk, and Vladimir Regions in 2000-2010 and those isolated in 1948—1999 (from the collection of the G.N. Gabrichevsky Moscow Institute of Epidemiology and Microbiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare). The investigation was performed sequencing ptxP gene fragments, followed by the analysis of nucleotide sequences, by applying the genomic and BLAST programs and EMBL/GenBank database (http://www.ncbi.nlm.nih.gov/entrez). The characterized B. pertussis strains were deposited in the collection of the G.N. Gabrichevsky Moscow Institute of Epidemiology and Microbiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare. The structural features of the ptxP gene encoding the pertussis toxin promoter region were studied and the time course of its change was traced in the B. pertussis strains isolated from whooping cough patients in different periods of the epidemic process of pertussis infection. The circulating B. pertussis strains were ascertained to have undergone substantial structural changes in this gene. There were strains carrying a novel non-vaccine ptxP3 allele that had contrast mutational changes involving the functional region responsible for the binding to the BvgA dimer, which led to an enhanced bonding strength and, as a consequence, to increased pertussis toxin production. The investigation has indicated that the B. pertussis population shows a predominance of strains with the novel non-vaccine ptxP3 allele and a high degree of virulence. The formation of a B. pertussis population follows as variability being in the genetic structure of B. pertussis strains with the emergence of novel non-vaccine alleles that have substantial mutational changes in the genes encoding both major pathogenicity factors and their regulatory structures that are responsible for the level of their expression.

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作者简介

O. BORISOVA

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

Email: olgaborisova@mail.ru

I. MAZUROVA

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

G. IVASHINNIKOVA

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

N. ZAKHAROVA

I.I. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow

N. MERTSALOVA

I.I. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow

E. ZAITSEV

I.I. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow

N. SALOVA

Branch, Center for State Sanitary-and-Epidemiological Surveillance in Moscow

F. ABASOVA

Branch, Center for State Sanitary-and-Epidemiological Surveillance in Moscow

I. TREBUNSKIKH

Branch, Center for State Sanitary-and-Epidemiological Surveillance in Moscow

V. SKACHKOVA

Branch, Center for State Sanitary-and-Epidemiological Surveillance in the Moscow North-Western Administrative District

R. PANFEROVA

Branch, Center for State Sanitary-and-Epidemiological Surveillance in the Moscow North-Western Administrative District

L. ALEKSEYEVA

Center for State Sanitary-and-Epidemiological Surveillance in the Vladimir Region

O. YAKUNINA

Center for State Sanitary-and-Epidemiological Surveillance in the Novosibirsk Region

N. NARETYA

Russian Inspectorate for the Protection of Consumer Rights and Human Welfare in Dzerzhinsk

M. VAN GENT

National institute for public health and the environment

F. MOOI

National institute for public health and the environment

V. ALESHKIN

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

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