Monitoring the pathogens (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Stenotrophomonas maltophilia) of healthcare-associated infections in a hospital

Objective. To identify the trends andfeatures of the distribution of P. aeruginosa, K. pneumoniae, A. baumannii, and S. maltophilia in hospital and the dynamics of their antibiotic resistance. Materials and methods. A total of 633 gram-negative bacterial isolates were studied. The bacteria were identified using biochemical tests and mass spectrometry; antibiotic resistance genes were determined by a polymerase chain reaction assay; antibiotic susceptibility was defined by diffusion methods. Results. The leading pathogens P. aeruginosa (n = 193), K. pneumoniae (n = 180), A. baumannii (n = 177), and S. maltophilia (n = 83) were identified in the bacterial profile of hospital. They showed a 2.8-fold preponderance in the intensive care units versus surgical units; the studied biomaterial displayed a predominance of monocultures in 79.1 % of cases; there was an uneven distribution of the pathogens in seasonal dynamics, their varying incidence according to gender, and non-specific colonization of patients (p < 0.05). Stable morphological types of antibiotic-resistant isolates were detected in A. baumannii (19.8%) and P. aeruginosa (19.7%). Conclusion. Local monitoring the leading pathogens of healthcare-associated infections makes it possible to obtain reliable data on their colonization of inpatients, on the characteristics of their distribution in seasonal and annual dynamics, and increased antibiotic resistance, which contributes to their timely consideration in a package of preventive and anti-epidemic measures implemented in a healthcare facility and promotes the maintenance of the eff iciency of its used antimicrobial agents.

P. aeruginosa, K. pneumoniae, A. baumannii, S. maltophilia, P. aeruginosa, K. pneumoniae, A. baumannii, S. maltophilia, monitoring