Oropharyngeal microbiota in HIV infection: trends and problems in the epoch of antiretroviral therapy


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Objective. To study the features of the oropharyngeal microbiota and systemic inflammation in HIV-infected patients during antiretroviral therapy (ART) and to assess the possibility of their correction with amino dihydrophthalazinedione sodium (ADPNa). Subjects and methods. The investigation enrolled 100 HIV-infected patients who received ART. The concentration of lipopolysaccharide-binding protein (LBP) was studied by ELISA. The composition of the oropharyngeal microbiota was determined on a VITEK-2 bacteriological analyzer by the compact disc diffusion method, as well as by mass spectrometry. The patients were randomized into 2 groups. In addition to standard ART, sublingual ADPNa tablets were prescribed for Group 1 patients. The eff iciency of therapy was evaluated after 4 weeks according to the time course of changes in the concentration of LBP and in the composition of the oropharyngeal microflora. Results. The vast majority of patients showed an overgrowth of opportunistic microorganisms, including Staphylococcus aureus and yeast-like fungi of the genus Candida. When an immunomodulator with an anti-inflammatory effect of ADPNa was added to standard ART, there was a more rapid decrease in the blood concentration of LBP and in the number of potential pathogens in the oropharyngeal microbiota. Conclusion. The cycle use of ADPNa reduces the activity of endotoxinemia and normalizes the oropharyngeal microbiota.

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作者简介

A. Matuzkova

Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: matuzkova@yandex.ru

N. Pshenichnaya

National Research Medical Center of Phthisiopulmonology and Infectious Diseases, Ministry of Health of Russia

Email: natalia-pshenichnaya@yandex.ru

A. Aleshukina

Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

A. Ryndich

Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: hivrost@mail.ru

A. Suladze

Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: hivrost@mail.ru

T. Tverdokhlebova

Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

A. Zhuravlev

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: Andrew0898@yandex.ru

D. Dontsov

Rostov State Medical University, Ministry of Health of Russia

Email: d-_dontcov@mail.ru

参考

  1. Ghannoum M. A., Mukherjee P. K., Jurevic R. J., Retuerto M., Brown R. E., Sikaroodi M., Webster-Cyriaque J., Gillevet P. M. Metabolomics reveals differential levels of oral metabolites in HIV-infected patients: toward novel diagnostic targets. Omics А Journal of Integrative Biology. 2013; 17(1): 5-15. DOI: 10.1089/ omi.2011.0035.
  2. Shiboski C. H., Patton L.L., Webster Cyriaque J.Y., Greenspan D., Traboulsi R.S., Ghannoum M., Jurevic R., Phelan JA., Reznik D., Greenspan J.S. The Oral HIV/AIDS Research Alliance: updated case definitions of oral disease endpoints. J. Ога1 Pathol. Med. 2009; 38(6): 481-8. doi: 10.1111/j.1600-0714.2009.00749.x.
  3. Dandekar S., George M.D., Baumler A.J. Th17 cells, HIV and the gut mucosal barrier. Current Gpinion in HIV and AIDS 2010; 5(2): 173-8. doi: 10.1097/COH.0b013e328335eda3.
  4. Sankaran S., George M.D., Reay E., Guadalupe M., Flamm J., Prindiville T., Dandekar S. Rapid onset of intestinal epithelial barrier dysfunction in primary human immunodeficiency virus infection is driven by an imbalance between immune response and mucosal repair and regeneration. J. Virol. 2008; 82 (1): 538-45. doi: 10.1128/JVI.01449-07.
  5. George M. D. Verhoeven D., Sankaran S., Glavan T., Reay E., Dandekar S. Heightened cytotoxic responses and impaired biogenesis contribute to early pathogenesis in the oral mucosa of simian immunodeficiency virus-infected rhesus macaques. Clin. Vaccine Immunol. 2009; 16(2): 277-81. DOI: 10.1128/ CVI.00265-08
  6. Крюков А.И., Кунельская Н.Л., Гуров А.В., Изотова Г.Н., Старостина А.Е., Лапченко А.С. Клиникомикробиологическая характеристика дисбиотических изменений слизистой оболочки полости рта и ротоглотки. Медицинский совет 2016; (6): 32-5
  7. Alexopoulou L., Kontoyiannis D. Contribution of microbial-associated molecules in innate mucosal responses. Cellular and molecular life sciences: CMLS 2005; 62(12): 1349-358. doi: 10.1007/s00018-005-5039-x
  8. Beck J. M., Beck J.M., Schloss P.D., Venkataraman A., Twigg I.H., Jablonski K.A., Bushman F.D. Multicenter comparison of lung and oral microbiomes of HIV-infected and HIV-uninfected individuals. Am. J. Respirat. Crit. Care Med. 2015; 192(11): 1335-44. doi: 10.1164/rccm.201501-0128OC
  9. Kistler J. O., Arirachakaran P., Poovorawan Y., Dahl6n G., Wade W.G. The oral microbiome in human immunodeficiency virus (HIV)-positive individuals. J. Med. Microbiol. 2015; 64(9): 1094-101. doi: 10.1099/jmm.0.000128.
  10. Saxena D., Li Y., Yang L., Pei Z, Poles M., Abrams W.R., Malamud D. Human microbiome and HIV/AIDS. Curr. HIV AIDS Rep. 2012; 9(1): 44-51. doi: 10.1007/s11904-011-0103-7.
  11. Mukherjee P.K., Chandra J., Retuerto M., Sikaroodi M., Brown R.E., Jurevic R., Salata R.A., Lederman M.M., Gillevet P.M., Ghannoum M.A. Oral mycobiome analysis of HIV-infected patients: identification of Pichia as an antagonist of opportunistic fungi. PLoS Fathogens 2014; 10(3): e1003996. doi: 10.1371/journal.ppat.1003996.
  12. Hegde M.C., Kumar A., Bhat G., Sreedharan S. Oral microflora: a comparative study in HIV and normal patients. Indian J. Otolaryngol. 2014; 66(1): 126-32. doi: 10.1007/s12070-011-0370-z.
  13. Dang A. T., Cotton S., Sankaran-Walters S., Li C., Lee C.M., Dandekar S., Paster B.J., George M.D. Evidence of an increased pathogenic footprint in the lingual microbiome of untreated HIV infected patients. BMC Microbiol. 2012; 12(1): 153. doi: 10.1186/1471-2180-12-153.
  14. Shiboski C. H., Lee A., Chen H., Webster-Cyriaque J., Seaman T., Landovitz R.J., John M., Reilly N., Naini L., Palefsky J., Jacobson M.A. Human papillomavirus infection in the oral cavity of HIV patients is not reduced by initiating antiretroviral therapy. AIDS (London, England). 2016; 30(10): 1573. doi: 10.1097/QAD. 0000000000001072.
  15. Noguera-Julian M. Guillen Y., Peterson J., Reznik D., Harris E.V., Joseph S. J. Oral microbiome in HIV-associated periodontitis. Medicine 2017; 96(12): e5821. DOI: 10.1097/ MD.0000000000005821.
  16. Liljestrand J. M., Paju S., Buhlin K., Persson G.R., Sarna S., Nieminen M.S. Lipopolysaccharide, a possible molecular mediator between periodontitis and coronary artery disease. J. ain. Рeriodontol. 2017; 44(8): 784-92. DOI: 10.1111/ jcpe.12751.
  17. Lew W.Y.W., Bayna E., Molle E. D., Dalton N.D., Lai N.C., Bhargava V., Mendiola V., Clopton P., Tang T. Recurrent exposure to subclinical lipopolysaccharide increases mortality and induces cardiac fibrosis in mice. PloS Ош 2013; 8(4): e61057. doi: 10.1371/journal.pone.0061057
  18. Бусленко А.О., Пшеничная Н.Ю., Алешукина А.В. Изменение состава микрофлоры кишечника при экспериментальной клебсиеллезной инфекции у лабораторных мышей и ее терапии различными иммуномодулирующими препаратами. Врач-аспирант 2015; 71(4.1): 139-44.
  19. Покровский А.В., Мхитарова Л.А., Царев В.Н. Прогностическое значение провоспалительных цитокинов при ингаляционном применении галавита в комплексном лечения ХОБ. Вестник Дагестанской государственной медицинской академии 2014; 1(10): 19-23
  20. Бусленко А.О., Алешукина А.В., Пшеничная Н.Ю. Хуказова С.А., Костенко О.Д., Гопаца Г.В., Малышева М.И.Местный цитокиновый профиль у взрослых пациентов с острыми кишечными инфекциями и его коррекция с применением иммуномодулирующей терапии. Эпидемиол. инфекц. болезни. Актуал. вопр. 2017; (4): 58-64
  21. Бусленко А.О., Пшеничная Н.Ю., Алешукина А.В., Усаткин А.В., Костенко О.Д., Хуказова С.А., Диденко И.В., Усаткина Т.В. Острые кишечные инфекции у взрослых: оценка состояния интестинальной микрофлоры и эффективности аминодигидрофталазиндиона натрия в составе их комплексной терапии. Эпидемиол. инфекц. болезни. Актуал. вопр. 2016; (4): 57-64
  22. Матузкова А.Н., Пшеничная Н.Ю., Суладзе А.Г., Досягаева Л.И., Твердохлебова Т.И., Яговкин Э.А. Клинико-диагностическое значение оценки показателей системного воспаления у больных с ВИЧ-инфекцией. ВИЧ-инфекция и иммуносупрессии 2018; 10(3): 64-71
  23. Джораева С.К., Гончаренко В.В., Щеголева Е.В., Щербакова Ю.В., Безрученко А.А. Состав и функции микробиоценозов различных биотопов макроорганизма и клиническая значимость их нарушений. Дерматология и венерология 2015; (2): 5-19

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