Development of a Microfluidic Fluorescent Chip Basis for Express Diagnostics Systems

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Abstract

Development of rapid and sensitive diagnostic methods is an actual issue of modern medicine. Current methods of diagnostics of myocardial infarction based on determination of enzyme activity (aspartate aminotransferase, creatine kinase, lactate dehydrogenase) have limitations due to low specificity. A unique direction in this field is the development of systems for express diagnostics of various pathologies based on microfluidic devices and their components – microchips. This approach uses the principle of a modified transparent matrix that allows photometric analysis by turbidimetry and
nephelometry methods. The work presents the development of a framework for a rapid diagnostic chip based on specific peptides and a fluorescent dye.

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About the authors

Evgeny A. Smirnov

Federal State Budgetary Institution V. A. Almazov National Medical Research Center of the Ministry of Health of the Russian Federation; FGAOU VO St. Petersburg State Electrotechnical University LETI named after V. I. Ulyanov (Lenin)

Author for correspondence.
Email: sea222777@yandex.ru
Russian Federation, St. Petersburg; St. Petersburg

Dmitry V. Korolev

Federal State Budgetary Institution V. A. Almazov National Medical Research Center of the Ministry of Health of the Russian Federation; Federal State Budgetary Educational Institution of Higher Education First Saint Petersburg State Medical University named after Academician I. P. Pavlov of the Ministry of Health of the Russian Federation

Email: dimon@cardioprotect.spb.ru
ORCID iD: 0000-0003-2848-3035
Russian Federation, St. Petersburg; St. Petersburg

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Supplementary files

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2. Fig. 1. Statistics of publications in the PubMed scientific database on the Biomarker sensor query

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3. Fig. 2. Binding of the indocyanine green dye to the surface of the aminated matrix

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4. Fig. 3. Investigation of chemical resistance of materials to 0.1 n NaOH. 1 – PET; 2 – glass-photoresist; 3 – PMMA; 4 – ABS plastic

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5. Fig. 4. ICZ chip: a – the adsorption process, b – the adsorption process is completed

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Copyright (c) 2025 Smirnov E.A., Korolev D.V.