STUDY FOR VASODILATORY AND ANTITHROMBOTIC FUNCTIONS OF ENDOTHELIUM IN THE CONDITION OF THE EXPERIMENTALLY INDUCED PATHOLOGIC SCAR

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The purpose of the work was to evaluate vasodilatory and antithrombotic functions of endothelium in the conditions of experimentally induced pathologic scar. Materials and methods: in this experi-ment we used 30 Wistar white male rats (aged 3 months, weighed 220-250 g), which had under-gone a two-week quarantine. The animals were divided into 2 groups. The first group included sham operated animals; the second group included a negative control. Modeling of the ex-perimental pathologic scar was carried out using a modified technique with animals anesthetized with a chlorhydrate narcosis (350 mg/kg).The evaluation of the recovery process and registra-tion of the indices were carried out on a 7th day of the runs. The study for vasodilatory function of an endothelium of cutaneous vessels was conducted by means of comparative testing of the endothelium by the reaction of cutaneous vessels and pathology. Registration of a blood flow was done in a dermal flap by using an invasive method from the inner side of skin by means of ultrasonic Doppler sonographer. A process of an aggregation of thrombocyte was registered with the use of two-channel laser analyzer of thrombocytes aggregation ALAT-2 BIOLA (NPF BIOLA, Russia). Statistic procession and analysis of the results of the runs were carried out by means of applied programs package, Microsoft Office Excel, StatPlus 2009 with the use of traditional techniques of variation deviations. Results of the study: the original speed of the local blood flow of skin of sham operated animals amounted to 2.366±0.24 cm/sec, and of rats with negative control to 2.136±0.12 cm/sec. The animals with pathologic scar do not have adequate response of endothelium of cutaneous vessels on the administration of acetylcholine (a blood flow reduced by 33% comparing with the initial data) while the administration of this me-diator to rats from the positive control group induced the blood flow accession by 47.6% (accurately, p=0.02), in terms of initial indices of blood flow speed. The administration of L-arginin to sham operated animals had smaller impact on the local dermal blood flow speed change, which amounted to 11.1% (in-accurately, p>0.05), while the blood flow of negative control rats reduced by 26%. After the administration of synthesis blocker NO - nitro - L - arginin of methyl ether to sham operated animals we noted the fall of an average systolic speed by 35.3% (accurately, p<0.02), while in pathologic animals fell only by 9.7%. Analyzing the data, obtained after the evaluation of antithrombotic function of endothelium, it is able to note the following: thrombocytes aggregation rate of negative control group was 2.41 higher than the rate of sham operated animals (p>0.01). Aggregation speed of sham operated animals amounted to 1.06±0.11 c.u. As the result of the pathology expansion this index raised by 183.3% (p>0.01) to the value of 3.003±0.49 c.u. Conclusions: in the setting of an operative treatment animals suffer the disorder of vasodilatory and antithrombotic function of an endothelium, which can be a precondition for a pathological scar formation.