PHENOLIC COMPOUNDS AND PECTIC SUBSTANCES OF EUROPEAN GOOSEBERRY LEAVES ΈGROSSULARIA RECLINATA ΈL.Ή MILL.Ή


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In the leaves of European gooseberry 23 compounds have been found, and only 13 of them have been identified by the presence of standards. One of the indicators of the bioavailability of drugs is the distribution coefficient of the substances under investigation between the hydrophilic and lipophilic phases, as well as in various biological fluids. Pectic substances have a preventive effect: anti-inflammatory, antibacterial, anti-sclerotic, anti-allergic. The aim of the work is: isolation, identification of phenolic compounds, investigation of the distribution coefficient and the antidislipidemic activity of the pectic substances in European gooseberry leaves (Grossularia reclinata (L.) Mill.). Materials and methods. Degreased raw materials (leaves of European gooseberry) were extracted three times with 40% ethanol by heating for 60 minutes. In the separatory funnel the combined extracts were subsequently treated with chloroform, diethyl ether, ethyl acetate and n-butanol. Isolation of pectin substances from the leaves of European gooseberry was carried out according to N.K. Kochetkov and M. Sinner’s method. When determining the distribution coefficient from organic solvents, diethyl ether and n-octanol, in which the objects under study are readily soluble, have been used. The procedures of the solutions input of the investigated pectic substances and cholesterol solution were separated by a 2-hour interval. After 14 days, venous blood was collected from the animals, followed by the production of serum and determination of the parameters characterizing the state of the lipid-cholesterol blood profile. Results and discussion. As a result of the study of phenolic compounds of European gooseberry leaves, hyperoside, rutin, caffeic acid have been separated for the first time. The data obtained show that for aqueous solutions of pectic substances of European gooseberry, the best distribution from the aqueous layer to the organic layer occurs in n-octanol. When using the pectic substances obtained from European gooseberry leaves, the normalization of the lipid-cholesterol profile in blood was observed in rats in comparison with the animals of the negative control group. Conclusion. By the method of preparative column chromatography, quercetin, hyperoside, rutin, kaempferol, astragaline, and caffeic acid were detected in European gooseberry leaves. It can be assumed that the pectic substances from gooseberry leaves will be better distributed and absorbed in the alkaline environment of intestinal contents. The use of the studied pectic substances from gooseberry leaves promoted the correction of dyslipidemia.

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121Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134121ɍȾК 582.711.31:547.56/.458.88:615.076.9 ˇʫʻОЛːʻˏʫʿʫК˃ʰʻОʦˏʫʦʫˍʫˁ˃ʦʤКˀˏʮОʦʻʰКʤО˃КЛОʻʫʻʻОʧОGROSSULARIA RECLINATA MILL.ȺɞжиɚɯмɟɬоɜɚПозɞнякоɜЧɟɪɜоннɚяМыкоцȼоɪонкоɜОɝɚнɟɫянмɟɞикоɮɚɪмɚцɟɜɬичɟɫкийинɫɬиɬɭɬɎȽȻОɍȼолɝоɝɪɚɞɫкийɝоɫɭɞɚɪɫɬɜɟнныймɟɞицинɫкийɭниɜɟɪɫиɬɟɬМинзɞɪɚɜɚɊоɫɫииɊоɫɫия, 11кɪыжоɜникɚоɬклонɟнноɝооɛнɚɪɭжɟно 23 ɫоɟɞинɟниякоɬоɪыɯɫɬɚнɞɚɪɬоɜиɞɟнɬиɮициɪоɜɚноɬолько 13 ɜɟщɟɫɬɜпокɚзɚɬɟлɟйɛиоɞоɫɬɭпноɫɬияɜляɟɬɫяэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияиɫɫлɟɞɭɟмыɯɜɟщɟɫɬɜмɟжɞɭɬɚкжɟɪɚзличныɯɛиолоɝичɟɫкиɯжиɞкоɫɬяɯПɟкɬиноɜыɟɜɟщɟɫɬɜɚоɛлɚɞɚюɬпɪоɮилɚкɬичɟɫкимɞɟйɫɬɜиɟмɬиɜоɜоɫпɚлиɬɟльнымɚнɬиɛɚкɬɟɪиɚльнымпɪоɬиɜоɫклɟɪоɬичɟɫкимɚнɬиɚллɟɪɝɟнɬнымЦɟльɪɚɛоɬыɜыɞɟлɟниɟиɞɟнɬиɮикɚцияɮɟнольныɯɫоɟɞинɟнийиɫɫлɟɞоɜɚниɟкоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияпиɞɟмичɟɫкойɚкɬиɜноɫɬипɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚоɬклонɟнноɝо (Grossularia reclinata (L.) МɚɬɟɪиɚлымɟɬоɞыОɛɟзжиɪɟнноɟкɪыжоɜникɚоɬклонɟнноɝоэкɫɬɪɚɝиɪоɜɚли 40% ɫпиɪɬомэɬилоɜымнɚɝɪɟɜɚнииɬɟчɟниɟ 60 ОɛъɟɞинɟнныɟизɜлɟчɟнияɞɟлиɬɟльнойɜоɪонкɟпоɫлɟɞоɜɚɬɟльнооɛɪɚɛɚɬыɜɚлиɯлоɪоɮоɪмомɞиэɬилоɜымэɮиɪомэɬилɚцɟɬɚɬомɛɭɬɚноломȼыɞɟлɟниɟпɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚоɬклонɟнноɝопɪоɜоɞилимɟɬоɞикɟКочɟɬкоɜɚ M. Sinner. опɪɟɞɟлɟниикоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияоɪɝɚничɟɫкиɯɪɚɫɬɜоɪиɬɟлɟйиɫпользоɜɚныɞиэɬилоɜыйэɮиɪокɬɚнолкоɬоɪыɯизɭчɚɟмыɟоɛъɟкɬыɯоɪошоɪɚɫɬɜоɪимыПɪоцɟɞɭɪыɜɜɟɞɟнияɪɚɫɬɜоɪоɜиɫɫлɟɞɭɟмыɯпɟкɬиноɜыɯɜɟщɟɫɬɜɪɚɫɬɜоɪɚɯолɟɫɬɟɪинɚɪɚзɞɟлɟны 2-чɚɫоɜыминɬɟɪɜɚломиɫɬɟчɟнии 14 жиɜоɬныɯпɪоизɜоɞилизɚɛоɪɜɟнознойпоɫлɟɞɭющимполɭчɟниɫыɜоɪоɬкиопɪɟɞɟлɟниɟмпɚɪɚмɟɬɪоɜɯɚɪɚкɬɟɪизɭющиɯɫоɫɬояниɟɯолɟɫɬɟɪиноɜоɝоɊɟзɭльɬɚɬыоɛɫɭжɞɟниɟɪɟзɭльɬɚɬɟиɫɫлɟɞоɜɚнияɮɟнольныɯɫоɟɞинɟнийкɪыжоɜниоɬклонɟнноɝоɜыɞɟлɟныɝипɟɪозиɞɪɭɬинкоɮɟйнɚякиɫлоɬɚПолɭчɟнныɟɫɜиɞɟɬɟльɫɬɜɭюɬɜоɞныɯɪɚɫɬɜоɪоɜпɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚлɭчшɟɟɪɚɫпɪɟɞɟлɟниɟɜоɞноɝоɫлояоɪɝɚничɟɫкийпɪоиɫɯоɞиɬокɬɚнолпɟкɬиноɜыɯɜɟщɟɫɬɜполɭчɟнныɯкɪыжоɜникɚжиɜоɬнымиконɬɪоляɝɪɭппынɚɛлюɞɚлɚɫьноɪмɚлизɚцияɯолɟɫɬɟɪиноɜоɝоЗɚключɟниɟМɟɬоɞомколоночнойɯɪомɚɬоɝɪɚɮииизɜлɟчɟниикɪыжоɜникɚоɬклонɟнноɝооɛнɚɪɭжɟныкɜɟɪцɟɬинɝипɟɪозиɞɪɭɬинкɟмпɮɟɪолкоɮɟйнɚякиɫлоɬɚМожнопɪɟɞположиɬьпɟкɬиноɜыɟɜɟщɟɫɬɜɚжоɜникɚщɟлочнойɫɪɟɞɟкишɟчноɝоɫоɞɟɪжимоɝоɛɭɞɭɬлɭчшɟɪɚɫпɪɟɞɟляɬьɫяɜɫɚɫыɜɚɬьɫяиɫɫлɟɞɭɟмыɯпɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚɫпоɫоɛɫɬɜоɜɚлокоɪɪɟкцииɞиɫлипиɞɟмииКлючɟɜыɟɫлоɜɚкɪыжоɜникɚоɬклонɟнноɝоɮлɚɜоноиɞыɮɟнолокиɫлоɬыполиɫɚɯɚɪиɞыкоэɮɪɚɫпɪɟɞɟлɟнияɞиɫлипиɞɟмияциɬиɪоɜɚнияȺɞжиɚɯмɟɬоɜɚПозɞнякоɜЧɟɪɜоннɚяМыкоцȼоɪонкоɜОɝɚнɟɫянɎȿНОЛЬНЫȿȼȿЩȿɋɌȼȺКɊЫЖОȼНИКȺ (GROSSULARIA RECLINATA (L.) MILL.). ɎɚɪмɚцияɮɚɪмɚколоɝияȺɞжиɚɯмɟɬоɜɚПозɞнякоɜЧɟɪɜоннɚяМыкоцȼоɪонкоɜОɝɚнɟɫянFor citation:Myikots L.P.,Voronkov A.V., Oganesyan E.T. PHENOLIC COMPOUNDS AND PECTIC SUBSTANCES OF EUROPEAN GOOSEBERRY LEAVES (GROSSULARIA RECLINATA (L.) MILL.).Pharmacy & Pharmacology.122ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝиякɪыжоɜникɚоɬклонɟнноɝомɟɬоɞомɜыɫокоэɮɮɟкɬиɜнойжиɞкоɫɬноймɚɬоɝɪɚɮииɪɭжɟно 23 ɫоɟɞинɟниякоɬоɪыɯнɚличиюɫɬɚнɞɚɪɬоɜиɞɟнɬиɮициɪоɜɚноɬолько 13 ɜɟщɟɫɬɜпɪɟɞɫɬɚɜлɟныɮлɚɜоноиɞɚмикɭмɚɪиɮɟнолкɚɪɛоноɜымикиɫлоɬɚми 13 Ⱦля ɛолɟɟпоɞɪоɛнойинɮоɪмɚцииполиɮɟнольномɫоɫɬɚɜɟизɜлɟчɟниякɪыжоɜникɚоɬклонɟнноɝоGrossularia reclinata (L.) Mill.лɭчɟнноɝоэкɫɬɪɚкциɟй 40% ɫпиɪɬомпɪоɜɟлиɟɝоɮɪɚкциониɪоɜɚниɟоɛɪɚɛоɬкойɪɚɫɬɜоɪиɬɟлямиɫочɟɬɚниипɪɟпɚɪɚɬиɜнойколоночнойɯɪомɚɬоɝɪɚɮиɟйОɞнимпокɚзɚɬɟлɟйɛиоɞоɫɬɭпноɫɬипɪɟпɚɪɚɬоɜяɜляɟɬɫякоэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияиɫɫлɟɞɭɟмыɯɜɟщɟɫɬɜɬɚкжɟɛиолоɝичɟɫкиɯжиɞкоɫɬяɯ [2].ПɟкɬиноɜыɟɜɟщɟɫɬɜɚоɛлɚɞɚюɬɬичɟɫкимпɪоɬиɜоɜоɫпɚлиɬɟльнымɛɚкɬɟɪиɚльнымпɪоɬиɜоɫклɟɪоɬичɟɫкимɚнɬиɚллɟɪPHENOLIC COMPOUNDS AND PECTIC SUBSTANCES OF EUROPEAN GOOSEBERRY LEAVES GROSSULARIA RECLINATA MILL.S.L. Adjiahmetova, D.I. Pozdnyakov, N.M. Chervonnaya, L.P. Myikots, A.V. Voronkov, E.T. OganesyanPyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State University 11, Kalinin ave., Pyatigorsk, Russia, 357532In the leaves of European gooseberry 23 compounds have been found, and only 13 of them have been identi ed by the presence of standards. One of the indicators of the bioavailability of drugs is the distribution coef cient of the sub-stances under investigation between the hydrophilic and lipophilic phases, as well as in various biological uids. Pec-tic substances have a preventive effect: anti-in ammatory, antibacterial, anti-sclerotic, anti-allergic. isolation, identi cation of phenolic compounds, investigation of the distribution coef cient and the antidis-lipidemic activity of the pectic substances in European gooseberry leaves (Grossularia reclinata (L.) Mill.). Degreased raw materials (leaves of European gooseberry) were extracted three times with 40% ethanol by heating for 60 minutes. In the separatory funnel the combined extracts were subsequently treated with chloroform, diethyl ether, ethyl acetate and n-butanol. Isolation of pectin substances from the leaves of European gooseberry was carried out according to N.K. Kochetkov and M. Sinner?s method. When determining the distribution coef cient from organic solvents, diethyl ether and n-octanol, in which the objects under study are readily soluble, have been used. The procedures of the solutions input of the investigated pectic substances and cholesterol solution were separated by a 2-hour interval. After 14 days, venous blood was collected from the animals, followed by the production of se-rum and determination of the parameters characterizing the state of the lipid-cholesterol blood pro As a result of the study of phenolic compounds of European gooseberry leaves, hyperoside, rutin, caffeic rst time. The data obtained show that for aqueous solutions of pectic substances of European gooseberry, the best distribution from the aqueous layer to the organic layer occurs in n-octanol. When using the pectic substances obtained from European gooseberry leaves, the normalization of the lipid-cholesterol pro- le in blood was observed in rats in comparison with the animals of the negative control group. By the method of preparative column chromatography, quercetin, hyperoside, rutin, kaempferol, astragaline, and caffeic acid were detected in European gooseberry leaves. It can be assumed that the pectic substances from gooseberry leaves will be better distributed and absorbed in the alkaline environment of intestinal contents. The use of the studied pectic substances from gooseberry leaves promoted the correction of dyslipidemia. European gooseberry leaves, avonoids, phenolic acids, polysaccharides, distribution coefɝɟнɬнымОɛлɚɞɚюɬɫɜойɫɬɜомкоɪɪɟкциилипиɞноɝооɛмɟнɚЦȿЛЬɊȺȻОɌЫиɞɟнɬиɮикɚцияɮɟнольныɯɫоɟɞинɟнийиɫɫлɟɞоɜɚниɟкоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияɚнɬиɞиɫлипиɞɟмичɟɫкойɚкɬиɜнопɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚоɬклонɟнноɝоGrossularia reclinata (L.) MillМȺɌȿɊИȺЛЫМȿɌОȾЫПолɭчɟниɟизɜлɟчɟнияɜыɞɟлɟниɟинɞиɜиɞɭɫоɟɞинɟнийкɚчɟɫɬɜɟиɫɫлɟɞɭɟмоɝоиɫпользоɜɚкɪыжоɜникɚоɬклонɟнноɝоиюлɟ 2015?2017 ɮɚзɭцɜɟɬɟнияɫоɪɬМоɫкоɜɫкийКɚɜкɚзɫкиɟМинɟɪɚльныɟȼоɞыɋɬɚɜɪопольОɛɟзжиɪɟнноɟкɪыжоɜникɚоɬклонɟнноɝоɬɪижɞыэкɫɬɪɚɝиɪоɜɚли 40% ɫпиɪɬомнɚɝɪɟɜɚнииɬɟчɟниɟ 60 Полɭчɟнноɟизɜлɟчɟниɟɫɝɭщɚлиоɛъɟмɚ 100 поɫлɟэɬоɝо123Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134123ɜоɪонкɟпоɫлɟɞоɜɚɬɟльнооɛɪɚɛɚɬыɜɚлиɯлоɪоɮоɪмомэɮиɪомэɬилɚцɟɬɚɬомɛɭɬɚноломПолɭчɟнныɟпоɫлɟɭɞɚлɟнияɪɚɫɬɜоɪипɟɪɟноɫилиɯɪомɚɬоɝɪɚɮичɟɫкиɟколонкизɚполнɟнныɟɫиликɚɝɟлɟммɚɪки 60 лиɚмиɞоммɚɪкиPolyamide WoelmЭлюɟнɬомɫлɭжилɚɜоɞɚочищɟннɚяɫиликɚɝɟляɯлоɪоɮоɪмПɪоцɟɫɫэлюиɪоɜɚнияконɬɪолиɪоɜɚлимɟɬоɞɚмиɬонкоɫлойнойɯɪомɚɬоɝɪɚɮииɛɭмɚжнойɯɪомɚɬоɝɪɚɮииMUNKTELL FN 7). кɚчɟɫɬɜɟпоɞɜижнойпользоɜɚлиɛɭɬɚнол : киɫлоɬɚɭкɫɭɫнɚя : ɜоɞɚȻɍȼкиɫлоɬɚɭкɫɭɫнɚяэɬилɚцɟɬɚɬ ? киɫлоɬɚɭкɫɭɫнɚяɜоɞɚȼыɞɟлɟниɟпɟкɬиноɜыɯɜɟщɟɫɬɜопɪɟɞɟлɟниɟкоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияпɟкɬиноɜыɯɜɟщɟɫɬɜжоɜникɚоɬклонɟнноɝопɪоɜоɞилимɟɬоɞикɟКочɟɬкоɜɚ M. Sinner [6]. ПоɫлɟɝиɞɪолизɚпɟкɬиноɜыɯɜɟщɟɫɬɜɪɭжɟныɝлюкозɚкɫиɝɚлɚкɬɭɪоноɜɚякиɫлоɬɚȽɪɚɜимɟɬɪичɟɫкийɚнɚлизɭкɚзыɜɚɟɬпɪɟоɛлɚɞɚниɟ (6,4%). Поэɬомɭɞɚльнɟйшиɟиɫɫлɟɞоɜɚнияпɪоɜоɞилиэɬой (6,4%). Пɪи опɪɟɞɟлɟниикоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияоɪɝɚничɟɫкиɯɪɚɫɬɜоɪиɬɟлɟйиɫпользоɜɚныэɮиɪокɬɚнолкоɬоɪыɯоɛъɟкɬыɯоɪошоɪɚɫɬɜоɪимыЭкɫɬɪɚкцияпɪоɜоɞилɚɫькɚкɜоɞныɯɪɚɫɬɜоɪоɜ (0,1-1%), ɬɚкɪɚɫɬɜоɪоɜɞоɜɟɞɟнныɯ pH=2 pH=8 ɪɚɫɬɜоɪоɜкиɫлоɬыɯлоɪиɫɬоɜоɞоɪоɞнойɝиɞɪокɫиɞɚнɚɬɪияɫооɬɜɟɬɫɬɜɟнноэкɫɬɪɚкцииоɛъɟмыиɫɫлɟɞɭɟмыɯɪɚɫɬɜоɪоɜэлɟкɬɪолиɬɚоɞинɚкоɜоКонцɟнɬɪɚцияɜоɞныɯɪɚɫɬɜоɪоɜнɚчɚльнɚяɪɚɮинɚɬɟопɪɟɞɟлялɚɫьконɞɭкɬомɟɬɪичɟɫкиммɟɬоɞомиɫпользоɜɚниɟмкɚлиɛɪоɜочноɝɪɚɮикɚзɚɜиɫимоɫɬи pH=8 ОпɪɟɞɟлɟниɟɚнɬиɞиɫлипиɞɟмичɟɫкойɚкɬиɜЖиɜоɬныɟОпɪɟɞɟлɟниɟɯолɟɫɬɟɪиноɜоɝоɜыполняли 30 кɪыɫɚɯ ? ɫɚмцɚɯWistarмɚɫɫой 200?220 ɪɚзɞɟлɟнныɯ 3 ɝɪɭппы (n=10). ɋоɞɟɪжɚниɟжиɜоɬныɯпɪоɜɟɞɟниɟэкɫпɟɪимɟнɬоɜɫооɬɜɟɬɫɬɜоɜɚлоɬоɪнойпɪɚкɬики (GLP) пɪоɜɟɞɟниииɫɫлɟɞоɜɚнийɊоɫɫийɫкойɎɟɞɟɪɚцииȽОɋɌ 51000.4-96), мɟжɞɭнɚɪоɞнымɪɟкомɟнȿɜɪопɟйɫкойконɜɟнциипозɜоночжиɜоɬныɯИɫɫлɟɞоɜɚнияпɟкɬиноɜыɯɜɟщɟɫɬɜɜыɞɟлɟнныɯкɪыжоɜникɚоɬклонɟннокоɪɪɟкциюɞиɫлипиɞɟмииПɟɪɜɚяɝɪɭппɚ ? инɬɚкɬныɟжиɜоɬныɟоɫɬɚɜшиɯɫямоɞɟлиɪоɜɚлиɝипɟɪɯолɟɫɬɟɪинɟкɭɪɫоɜоɝо (14 пɟɪоɪɚльноɝоɜɜɟɞɟ 3% ɪɚɫɬɜоɪɚɯолɟɫɬɟɪинɚɫɟɪияпоɞɫолнɟчноммɚɫлɟ [8]. эɬомɜɬоɪɭюɝɪɭппɭɫоɫɬɚɜилижиɜоɬныɟнɟɝɚɬиɜноɝоконɬɪоляполɭчɚɜшиɟɮɚɪмɚколоɝичɟɫкɭюпоɞɞɟɪжкɭɌɪɟɬьɟйɝɪɭппɟɜɜоɞилииɫɫлɟɞɭпɟкɬиноɜыɟɜɟщɟɫɬɜɚкɪыжоɜникɚоɬклонɟнноɝоколичɟɫɬɜɟ 1 ɪɚɫɬɜоɪɚɜɟɫɚжиɜоɬноɝоПɪоцɟɞɭɪыɜɜɟɞɟнияɪɚɫɬɜоɪоɜиɫɫлɟɞɭɟмыɯɜɟщɟɫɬɜɪɚɫɬɜоɪɚɯолɟɫɬɟɪинɚɪɚзɞɟ 2-инɬɟɪɜɚломиɫɬɟчɟнии 14 жиɜоɬныɯпɪоизɜоɞилиɜɟныпоɫлɟɞɭющимполɭчɟниɟмɫыɜоɪоɬкиопɪɟɞɟлɟнипɚɪɚмɟɬɪоɜɯɚɪɚкɬɟɪизɭющиɯɫоɫɬояниɟɯолɟɫɬɟɪиноɜоɝооɛщɟɝоɯолɟɫɬɟɯолɯолɟɫɬɟɪинɚлипопɪоɬɟиɞоɜнизкойплоɬноɫɬиɯолɯолɟɫɬɟɪинɚлипопɪоɬɟиɞоɜɜыɫокойплоɬноɫɬиɯолɬɪиɝлицɟɪиɞоɜɪɚɛоɬɟиɫпользоɜɚлиɫьɫɬɚнɞɚɪɬныɟɪɟɚкɬиɜоɜпɪоизɜоɞɫɬɜɚОльɜɟкɫȾиɚɝноɫɬикɭмɯолɟɫɬɟɪинɫɟɪия 01042017, ɯолɟɫɬɟɪинɫɟɪия 080022017, ɯолɟɫɬɟɪинɫɟɪия 20022017, ɬɪиɝлицɟɪиɞыɫɟɪия 01122016, ШɜɟцияПɪоɛопоɞɝоɬоɜкɚɞɚльнɟйшийɯоɞɚнɚлизɚɫооɬɜɟɬɫɬɜоɜɚлиинɫɬɪɭкциикɚжɞомɭнɚɛоɪɭɋɬɚɬиɫɬичɟɫкɚяоɛɪɚɛоɬкɚɊɟзɭльɬɚɬыэкɫпɟɪимɟнɬɚоɛɪɚɛɚɬыɜɚлимɟɬоɞɚɜɚɪиɚционнойɫɬɚɬиɫɬикииɫпользоɜɚниɟмпɚкɟ STATISTICA 6.0 (ПолɭчɟнныɟпоɞɜɟɪɝɚлиɬɟɫɬɭмɚльноɫɬьɪɚɫпɪɟɞɟлɟнияиɫпользоɜɚниɟмШɚпиɪоɍилкɚпоɞчинɟниязɚконɭноɪмɚльноɝоɪɚɫпɪɟɞɟлɟнияɫɪɟɞниɯ t-ɋɬьюɞɟнɬɚпɪоɬиɜномɞɚльнɟйшɭюɫɬɚɬиɫɬичɟɫкɭюоɛɪɚɛоɬкɭпɪоизɜоɞилипомощьюɍиɬниɊȿЗɍЛЬɌȺɌЫОȻɋɍЖȾȿНИȿȼыɞɟлɟниɟинɞиɜиɞɭɚльныɯɜɟщɟɫɬɜмɟɬоɞомколоночнойɯɪомɚɬоɝɪɚɮииɏлоɪоɮоɪмнɭюпɟɪɟноɫиликолонкɭполиɚмиɞомэлюиɪоɜɚлипоɫлɟɞоɜɚɬɟльноɜоɞойочищɟннойɫпиɪɬомконцɟнɬɪɚцииɫпиɪɬом 95%. Полɭчɟныɞɜɚɜɟщɟɫɬɜɚоɞинкоɬоɪыɯɯɚɪɚкɬɟɪизɭɟɬɫяɝолɭɛымɫɜɟчɟниɟмɜɟщɟɫɬɜоɜɬоɪоɟɛɭɪɭюокɪɚɫкɭзоныɫɜɟɬɟɜɟщɟɫɬɜоэɮиɪколонкɟɫиликɚɝɟлɟмɞɜɚɜɟщɟɫɬɜɚ ? ɜɟщɟɫɬɜоɜɟщɟɫɬɜоɫɜɟɬɟзонɭɛɭɪоɝоцɜɟɬɚЭɬилɚцɟɬɚɬнɭюɫɝɭщɚлинɟɛольшооɛъɟмɚɫмɟшиɜɚлиɛɟзɜоɞнымɯлоɪоɮоɪмомоɫɚɞокпɟɪɟноɫиликолонкɭполиɚмиɞомэлюиɪоɜɚлиɜоɞноэɬɚнольнымиɭɜɟличɟниɟмɫпиɪɬɚэɬилоɜоɝополɭчилиɜɟщɟɫɬɜɚɏлоɪоɮоɪмноɬɚɬныйɮильɬɪɚɬпоɫлɟоɬɞɟлɟнияоɫɚɞкɚɫɝɭщɚлипɟɪɟноɫиликолонкɭɫиликɚɝɟлɟмЭлюиɪоɜɚниɟмɯлоɪоɮоɪмноэɬɚнольнымиɫмɟɫямиɭɜɟличɟниɟмɫпиɪɬɚэɬилоɜоɝополɭчɟныɞɜɟоɞнойɫоɞɟɪжɚɬɫяɞɜɚɫоɟɞинɟнияполиɮɟнольнойпɪиɪоɞыɜɟщɟɫɬɜɚɜɬоɪой124ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияɜɟщɟɫɬɜɚ ? ɜɟщɟɫɬɜɚ11 ȾɚлɟɟоɛɟпоɞɜɟɪɝлипоɜɬоɪномɭɪɚзɞɟлɟниюколонкɚɯполиɚмиɞомЭлюиɪоɜɚниɟоɫɭщɟɫɬɜлялиɜоɞноэɬɚнольнымиɫмɟɫямиɏɪомɚɬоɝɪɚɮичɟɫкийɚнɚлизмɚлыйинɬɟɪɜɚлɬɟмпɟɪɚɬɭɪплɚɜлɟнияɜɟщɟɫɬɜɬɟльɫɬɜɭɟɬɬомчɬояɜляюɬɫяинɞиɜиɞɭɚльныɬɚɛл ɌɚɛлицɚЗнɚчɟниякоэɮɮициɟнɬоɜпоɞɜижноɫɬиполɭчɟнныɯɜɟщɟɫɬɜЗнɚчɟниɟкоэɮɮициɟнɬɚпоɞɜижноɫɬиНомɟɪполɭчɟнноɝоɜɟщɟɫɬɜɚкиɫлоɬɚɭкɫɭɫнɚяȻɍȼэɬилɚцɟɬɚɬкиɫлоɬɚɭкɫɭɫнɚяɜоɞɚ0,36?0,020,42?0,030,44?0,030,59?0,040,64?0,020,32?0,020,26?0,020,78?0,040,83?0,0450,52?0,030,77?0,030,80?0,0370,43?0,020,73?0,020,64?0,0280,07?0,010,89?0,030,90?0,0210ɫпɟкɬɪɜɟщɟɫɬɜɚɬɚкжɟɬɟльноɝоɯɪомɚɬоɝɪɚɮичɟɫкоɝоɚнɚлизɚɬɪоɮоɬомɟɬɪииионизиɪɭющимикомплɟкɫооɛɪɚзɭющимиɞоɛɚɜкɚмиɚнɚлоɝичнымпɚɪɚмɟɬɪɚмɜɟщɟɫɬɜɚэɬомɫɜиɞɟɬɟльɫɬɜɭɬɚкжɟоɬɫɭɬɫɬɜиɟɞɟпɪɟɫɫииɬɟмпɟɪɚɬɭɪыплɚɜлɟпɪоɛɟȼыяɜлɟнɚɫɯожɟɫɬьнɟкоɬоɪыɯɮизикоɯимичɟɫкиɯпɚɪɚмɟɬɪоɜɜɟщɟɫɬɜȼɟщɟɫɬɜɚпɪиоɛɪɟɬɚюɬкɪɚɫноɟшиɜɚниɟциɚниɞиноɜойчɬоɞɟɬɟльɫɬɜɭɟɬпɪинɚɞлɟжноɫɬипɪоизɜоɞнымɮлɚɜонолɚȺнɚлизɫɬɪɭкɬɭɪɫоɟɞинɟнийоɫɭщɟɫɬɜлялимɟɬоɞомɫпɟкɬɪоɫкопииионизиɪɭюкомплɟкɫооɛɪɚзɭющимиɞоɛɚɜкɚмипоɫкольмɚкɫимɚльноинɮоɪмɚɬиɜнɚɞоɫɬоɜɟɪнɚ10ɍɎ-ɫпɟкɬɪыɚɝликоноɜɜɟщɟɫɬɜɚɯɚɪɚкɬɟɪизɭюɬɫянɚличиɟмɞɜɭɯполоɫоɞинɚкоɜойинɬɟнɫиɜноɫɬиПолɭчɟнныɟɫɜиɞɟɬɟльɫɬɜɭɬомчɬоɫоɟɞинɟнийположɟниипɪиɫɭɬɫɬɜɭɟɬɫɜоɛоɞнɚяɝиɞɪокɫиɝɪɭппɚчɬоɭкɚзыɜɚɟɬзнɚчиɬɟльныйɛɚɬоɯɪомныйоɛɟиɯполоɫпоɝлощɟнияпɪиɫɭɬɫɬɜииɚлюминияȼɟщɟɫɬɜɚпɪиɫɭɬɫɬɜииɚлюминияɞɚюɬзнɚчиɬɟльныйɛɚɬоɯɪомныйоɛɟиɯполоɫчɬоɫɜиɞɟɬɟльɫɬɜɭɟɬнɚличииɝиɞɪокɫиɝɪɭппɞоɛɚɜлɟнииɫɜɟжɟплɚɜлɟнноɝонɚɬɪияɚцɟɬɚɚнɚлизиɪɭɟмымɪɚɫɬɜоɪɚмɜɟщɟɫɬɜнɚɛлюɞɚɟɬɫяɛɚɬоɯɪомныйкоɪоɬкоɜолноɜой II полоɫыэɬоɫɜиɞɟɬɟльɫɬɜɭɟɬнɚличииɫɜоɛоɞнойɝиɞɪокɫиɝɪɭппыɜɫɟɯɫоɟɞинɟнийȻɚɬоɯɪомɞлинноɜолноɜойполоɫыɫпɟкɬɪɚɯщɟɫɬɜɜызыɜɚɟɬɞоɛɚɜлɟниɟɫɜɟжɟплɚɜлɟнноɚцɟɬɚɬɚнɚɬɪиякиɫлоɬычɬоɯɚɪɚкɬɟɪноɮлɚɜоноиɞоɜоɪɬоɞиɝиɞɪокɫиɝɪɭппиɪоɜкɭположɟнияɯ 3? 4? ɛокоɜоɝоɮɟнильноɝоɪɚɞикɚлɚȻɚɬоɯɪомный I-полоɫыɛɟзɫнижɟнияинɬɟнɫиɜноɫɬинɚɛлюɞɚɟɬɫяпоɞɜлияниɟммɟɬилɚɬɚнɚɬɪиячɬоɭкɚзыɜɚɟɬнɚличиɟɝиɞɪокɫиɝɪɭппɚɬомоɜɫнижɟниɟинɬɟнɫиɜноɫɬичɟɪɟз 3-5 ɜɟщɟɫɬɜɬɚкжɟнɚɛлюɞɚɟɬɫяɛɚɬоɯɪомный I-полоɫыɛɟзизмɟнɟнияинɬɟнɫиɜноɫɬичɬоɫɜиɞɟɬɟльɫɬɜɭɟɬɫɜоɛоɞнойɝиɞɪокɫиɝɪɭппɟɫоɟɞинɟнияɯ I-Ⱦля ɭɬочнɟнияположɟнияɭɝлɟɜоɞноɝооɫɬɚɬкɚиɫпользоɜɚлиɫпɟкɬɪоɫкопиюɚɝликонɚпɪиɫɭɬɚлюминияɫпɟкɬɪоɜпоɝлощɟнияɜɟщɟɫɬɜпоɫлɟɝиɞɪолизɚɫɟɛяɜнимɚниɟпояɜлɟниɟɛɚɬоɯɪомноɝоɚɝликонɚчɬооɫноɜɚниɟɭɬɜɟɪжɞɚɬьчɬоɫоɟɞинɟнияɯмɟɫɬоɝликозиɞиɪоɜɚниɟположɟниюɫпɟкɬɪыизмɟɪялиɮɭɪьɟɫпɟкɬɪомɟɬɪ 1201?. Кɚɪɛонильнɚяɝɪɭппɚзɚмɟщɟнноɝоɮлɚɜонɚпоɝлощɚɟɬ 1650 ɮлɚɜонɫоɞɟɪжиɬɝɪɭппɭположɟниичɚɫɬоɬɚпонижɚɟɬɫя 1619 ȼɜɟɞɟниɟɝɪɭппыположɟнииɮлɚɜоноиɞоɜɫколькоɭɜɟличиɜɚɟɬчɚɫɬоɬɭпоɝлощɟниякɚɪɛонильɝɪɭппы 1655 нɟɫмоɬɪяɜозможноɫɬьоɛɪɚзоɜɚнияɜнɭɬɪимолɟкɭляɪнойɜоɞоɪоɞнойɫɜязи 1655 ȼ ɪɟзɭльɬɚɬɟщɟлочноɝоиɫɫлɟɞɭɚɝликоноɜкɚлияɝиɞɪокɫиɞомполɭчɟныпɪоɬокɚɬɟɯоɜɚякиɫлоɬɚɮлоɪоɝлюцинɪɟзɭльɬɚɬɟщɟлочноɝоɚɝликоноɜкɚлияɝиɞɪокɫиɞомполɭчɟныɝиɞɪокɫиɛɟнзойнɚякиɫлоɬɚɮлоɪоɝлюцинчɬоɞокɚзɚнопоɞɜижнɚя ? Ȼɍȼ (4:1:5), пɪояɜиɬɟль ? 1% ɪɚɫɬɜоɪжɟлɟзɚ (III), 0,1% ɞиɚзоɬиɪоɜɚннɚяɫɭльɮɚнилоɜɚякиɫлоɬɚ (III), 0,1% ȼ ɪɟзɭльɬɚɬɟполноɝокиɫлоɬноɝоɝиɞɪолизɚщɟɫɬɜɚполɭчɟныɚɝликон ? кɜɟɪцɟɬинɝɚлɚкɬозɚȽиɞɪолизɚɬоɛɪɚɛɚɬыɜɚлиэɮиɪомɜоɪонкɟМɟɬоɞомпользоɜɚниɟмɫɬɚнɞɚɪɬноɝо ? кɜɟɪцɟɬинɚпоɞɜижныɯȻɍȼ (4:1:5) 15% ɭкɫɭɫнойлоɬыɯɪомɚɬоɝɪɚɮиɪоɜɚлиполɭчɟнноɟизɜлɟчɟниɟȽиɞɪолизɚɬнɟйɬɪɚлизоɜɚликɚɪɛонɚɬомɭниɜɟɪɫɚльномɭинɞикɚɬоɪɭɮильɬɪоɜɚлиɭпɚɪиɜɚнɟɛольшоɝооɫɬɚɬкɚɯɪомɚɬоɝɪɚɮиɪоɜɚлипоɞɜижнойȻɍȼ (4:1:5) иɫпользоɜɚниɟмɫɬɚнɞɚɪɬныɯПоɫлɟпɪояɜлɟнияɚнилинɮɬɚлɚɬɪɟɚкɬиɜомоɛнɚɪɭжилиɝɚлɚкɬозɭ (4:1:5) ȼ ЯМɊ-1Н-ɫпɟкɬɪɟ (400MHz, DMSO-dɜɟщɟɫɬɜɚпɪояɜляюɬɫяɫиɝнɚлыпɪоɬоноɜ125Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134125ɋпɟкɬɪɜɟщɟɫɬɜɚ); 115,58 (Ɍɚкимоɛɪɚзомɫпɟкɬɪоɫкопиипоɞɬɜɟɪжɞɚюɬɜыɜоɞыполɭчɟнныɟоɫноɜɚнииɫпɟкɬɪоɮоɬомɟɬɪииионизиɪɭющикомплɟкɫооɛɪɚзɭющимиɞоɛɚɜкɚмиɫпɟкɬɪɜɟщɟɫɬɜɚɯɚɪɚкɬɟɪизɭɟɬɫяɬɪɟмялоɫɚмипоɝлощɟниямɚкɫимɭмɚми 216, 243, 325 ɫпɟкɬɪоɫкопииɛɭмɚжнойɯɪомɚɬоɝɪɚɮииможноɫɞɟлɚɬьɜыɜоɞчɬоɜɟщɟɫɬɜоэɬокоɮɟйнɚякиɫлоɬɚ 216, 243, 325 Полɭчɟнныɟɪɟзɭльɬɚɬыɫɜиɞɟɬɟльɫɬɜɭюɬчɬоɜɟщɟɫɬɜо ? эɬо 3,5,7,3?,4?-пɟнɬɚокɫиɮлɚɜонкɜɟɪɜɟщɟɫɬɜо ? кɜɟɪцɟɬинɝɚлɚкɬоɫоɟɞинɟниɟкɜɟɪцɟɬинɪɭɬиɪɭɬинɫоɟɞинɟниɟпɪɟɞɫɬɚɜляɟɬɬɟɬɪɚокɫиɮлɚɜонкɟмпɮɟɪолɜɟщɟɫɬɜокɟмпɮɟɪолɝлюкопиɪɚнозиɞɚɫɬɪɚɝɚлинɜɟщɟɫɬɜокоɮɟйнɚякиɫлоɬɚОпɪɟɞɟлɟниɟкоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияКоэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияокɬɚнолɜоɞɚочищɟннɚяшиɪокоиɫпользɭɟɬɫямɟɞицинɟɮɚɪмɚколоɝииопиɫɚнияɬɪɚнɫпоɪɬɚɜɟщɟɫɬɜчɟɪɟзɛиолоɝичɟɫкиɟ ? Покɚзɚночɬозɚɜиɫимоɫɬькоэɮɮициɟнɬомɪɚɫпɪɟɞɟлɟнияɛиолоɝичɟɫкимможɟɬноɫиɬьпɚɪɚɛоличɟɫкийɯɚɪɚкɬɟɪэɬомɞоɫɬижɟнииопɬимɭмɚлипоɮильноɫɬиɞɚльнɟйшɟɟможɟɬпɪиɜоɞиɬьɫнижɟниюɛиолоɝичɟɫкойɚкɬиɜноɫɬи [17]. ɊɟзɭльɬɚɬыопɪɟɞɟлɟниякоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияпɪɟɞɫɬɚɜлɟныɬɚɛлицɚɯɌɚɛлицɚКоэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияɫиɫɬɟмɚɯɜоɞɚочищɟннɚяокɬɚнолɜоɞɚочищɟннɚяɞиэɬилоɜыйэɮиɪиɫɯоɞноɟэкɫɬɪɚкцияэɮиɪом0,10,420,370,070,030,20,750,710,180,020,41,391,050,290,11экɫɬɪɚкцияокɬɚнолом0,61,951,890,590,010,82,582,470,780,021,03,172,870,900,10Пɪимɟчɚниɟ ? иɫɯоɞнɚяконцɟнɬɪɚцияɫопɪоɬиɜлɟниɟɜоɞныɯɪɚɫɬɜоɪоɜ ? ɪɚɜноɜɟɫныɟконцɟнɬɪɚɫɬɜоɪоɜпоɫлɟэкɫɬɪɚкцииɌɚɛлицɚɫɪɟɞыкоэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияɫиɫɬɟмɟɜоɞɚочищɟннɚяокɬɚнолиɫɯоɞноɟиɫɯоɞноɟокɬɚнолиɫɯоɞноɟокɬɚнол0,60,986,866,573,433,100,570,590,81,786,856,412,592,480,750,761,02,186,724,063,182,450,600,77КоэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияɪɚɫɫчиɬыɜɚличɟɪɟзɪɚɜноɜɟɫныɟконцɟнɬɪɚцииɜоɞɟочиокɬɚнолɟКонцɟнɬɪɚцияɬɚнольноɝоɪɚɫɬɜоɪɚɜозɜоɞилɚɫьоɬɜɟɬɫɬɜɭющɟйПоɫлɟɞняяопɪɟɞɟлялɚɫьɝɪɚɮичɟɫкимɫпоɫоɛомɪиɫɭнкɟ 1 покɚзɚнɚлинɟйноɫɬьɬɚкоймоɫɬипозɜоляющɚяɝɪɚɮичɟɫкиопɪɟɞɟлиɬьɜɟличинɭкоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияПолɭчɟнныɟɫɜиɞɟɬɟльɫɬɜɭюɬчɬоɪɚɫɬɜоɪоɜкɪыжоɜникɚɪɚɫпɪɟɞɟлɟниɟɜоɞноɝоɫлояоɪɝɚничɟɫкийпɪоиɫɯоɞиɬокɬɚнолЭкɫпɟɪимɟнɬɚльныɟɜлиянииɪɚɫпɪɟɞɟлɟниɟɫɪɟɞыпокɚзɚличɬокɪыжоɜникɚпɪɟɞɟлɟниɟпɪоиɫɯоɞиɬщɟлочнойɫɪɟɞɟɬɚɛлɌɚɛлицɚЗнɚчɟниякоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияɪɚзличныɯɫɪɟɞɚɯ№Коэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияɋɪɟɞɚ11,59ɜоɞɚочищɟннɚяокɬɚнол22,60ɜоɞɚочищɟннɚяэɮиɪ31,84ɜоɞɚочищɟннɚяокɬɚнол41,43ɜоɞɚочищɟннɚяокɬɚнол126ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияɊиɫɭнокȽɪɚɮичɟɫкийɪɚɫчɟɬпɚɪɚмɟɬɪоɜɪɚɫпɪɟɞɟлɟниякɪыжоɜникɚоɬклонɟнноɝоПɪимɟчɚниɟɪɚɜноɜɟɫныɟконцɟнɬɪɚцииɪɚɫɬɜоɪоɜпоɫлɟэкɫɬɪɚкцииПокɚзɚночɬопɪоцɟɫɫпɟɪɟɯоɞɚɫкɭюɮɚзɭокɬɚнолноɫиɬэкзоɬɟɪмичɟɫкийɯɚɪɚкизмɟнɟнииизоɛɚɪноизоɬɟɪмичɟɫкопоɬɟнциɚлɚэнɬɚльпийнойɫоɫɬɚɜляющɟйɛольшɟэнɬɪопийноɝоɮɚкɬоɪɚпоэɬомɭЭɬоɫɜиɞɟɬɟльɫɬɜɭɟɬɫɚмопɪоизɜольноɫɬипɪоцɟɫɫɚɪɚɫпɪɟɞɟлɟнияполиɫɚɯɚɪиɞоɜоɪɝɚничɟɫкийпɟкɬиноɜыɯɜɟщɟɫɬɜɜыɞɟлɟнныɯкɪыжоɜникɚоɬклонɟнноɝоɯолɟɫɬɟɪиноɜыйɝɪɭппыинɬɚкɬныɯɫоɞɟɪжɚниɟоɛщɟɝолɟɫɬɟɪинɚɯолɟɫɬɟɪинɚɯолɟɫɬɟɪинɚɫоɫɬɚɜляло 2,42?0,08 ммольммоль 0,47?0,03 ммольɫооɬɜɟɬɫɬɜɟнноɊиɫɭнокɯолɟɫɬɟɪиноɜыйПɪимɟчɚниɟоɛщоɛщийɯолɟɫɬɟɪин ? ɯолɟɫɬɟɪиннизкойплоɬноɫɬиɯолɟɫɬɟɪинɜыɫокойплоɬноɫɬи ? ɬɪиɝлицɟɪиɞы ? инɬɚкɬныɟжиɜоɬныɟ ? конɬɪольпɟкɬиноɜыɟɜɟщɟɫɬɜɚɜыɞɟлɟнныɟкɪыжоɜникɚоɬклонɟнноɝоɫɬɚɬиɫɬичɟɫкизнɚчимоɋɬьюɞɟнɬɚоɬноɫиɬɟльноɝɪɭппыжиɜоɬныɯɫɬɚɬиɫɬичɟɫкизнɚчимоɋɬьюɞɟнɬɚоɬноɫиɬɟльноɝɪɭппыжиɜоɬныɯ127Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134127 By methods of high-perfor- ed by the presence of standards. avonoids, coumarins and phe-avonoids, coumarins and phe-For more detailed information on the polyphenol ex-tract from European gooseberry leavesGrossularia rec- (L.) Mill.), obtained by extraction with 40% ethyl chromatography. cient of the substances under study uids [2].Pectic substances (PS) have a preventive effect: an- ammatory, antibacterial, anti-sclerotic, anti-aller-gic. They have the property of lipid metabolism correc-They have the property of lipid metabolism correc-THE AIM OF THE WORK is isolation, identi cient and the antidislipidemic activity of the Gros-sularia reclinataMATERIALS AND METHODSEuropean gooseberry leaves collected in July-August owering and fruiting, the ?Mos-cow red? variety (the region of the Caucasian Mineral Wa-ters (Stavropol Territory)) were used as the test material. by heating for 60 minutes. The resulting recovery was treated with chloroform, diethyl ether, ethyl acetate and tions were transferred to chromatographic columns lled with silica gel of the Silicagel 60 brand or poly-amide of the Polyamide Woelm brand. The eluent for ed water, and for silica gel it was chloroform. The elution process was monitored by l? plates) and paper chromatography (PC) (MUNKTELL FN 7). As the mobile phase n-butanol: acetic acid: water (BAW ter (5: 1: 1) were used. cient substances from European goose-kov and M. Sinner?s methods [6]. After hydrolysis, glu-ysis indicated PS predominance (6.4%). Therefore, fur--When determining the distribution coef? cient from organic solvents, diethyl ether and n-octanol were used, экɫпɟɪимɟнɬɚльнойɝипɟɪɯолɟɫɬɟɪинɟмиижиɜоɬныɯɝɪɭппынɟɝɚɬиɜноɝоконɬɪоляинɬɚкɬнымикɪыɫɚмиоɬмɟчɟноконцɟнɬɪɚцииоɛщɟɝоɯолɟɫɬɟɪинɚɯолɟɫɬɟ 95,5% (p)() 110,6% (p)ɫооɬɜɟɬɫɬɜɟнноɋоɞɟɪжɚниɟлɟɫɬɟɪинɚɝɪɭппыжиɜоɬныɯнɚпɪоɬиɜɭмɟньшилоɫьоɬношɟниюкɪыɫɚм 86,5% чɬоɫоɝлɚɫɭɟɬɫялиɬɟɪɚɬɭɪными 86,5% Поɞпɟкɬиноɜыɯɜɟщɟɫɬɜполɭчɟнкɪыжоɜникɚжиɜоɬнымиɝɪɭппынɚɛлюɞɚлɚɫьноɪмɚлизɚцияɯолɟɫɬɟɪиноɜоɝоɜыɪɚжɚɟмɚяɫнижɟнииконцɟнɬɪɚцииоɛщɟɝоɯолɟɯолɟɫɬɟɪинɚ 27,1% (p)ɫооɬɜɟɬɫɬɜɟнноɬɚкɫоɞɟɪжɚнияɯолɟɫɬɟɪинɚПолɭчɟнныɟɯоɞɟиɫɫлɟɞоɜɚнияɝлɚɫɭюɬɫялиɬɟɪɚɬɭɪыпоɞɬɜɟɪжɞɚюɬɛлɚɝопɪияɬноɟɜлияниɟпɟкɬиноɜыɯɜɟщɟɫɬɜɯолɟɫɬɟɪиноɜыйоɛмɟн [18]. ɋнижɟниɟоɛщɟɝоɯолɟɫɬɟɪинɚɬɪиɝлицɟɪиɞоɜноɪмɚлизɚцияоɬношɟнияɯол / ɯолɜɜɟɞɟнияизɭчɚɟмыɯпɟкɬиноɜыɯɜɟщɟɫɬɜɜозможноɫɜязɚнопɪоцɟɫɫɚɜɫɚɫыɜɚнияизɛыɬкɚɯолɟɫɬɟɪиɬɪиɝлицɟɪиɞоɜчɬоɫɜоюочɟɪɟɞьпɪɟɞоɬɜɪɚпɪоɚɬɟɪоɝɟнныɟэɮɮɟкɬы / ЗȺКЛЮЧȿНИȿМɟɬоɞомпɪɟпɚɪɚɬиɜнойколоночɯɪомɚɬоɝɪɚɮииизɜлɟчɟниикɪыжоɜникɚоɬклонɟнноɝоGrossularia reclinata (L.) Mill.полɭчɟнноɝоɫпиɪɬом 40%, ɪɭжɟныкɜɟɪцɟɬинɪɭɬинкɟмпɮɟɪолɚɫɬɪɚɝɚлинкоɮɟйнɚякиɫлоɬɚȽипɟɪозиɞɪɭɬинкоɮɟйнɚякиɫлоɬɚкɪыжоɜникɚоɬклонɟнноɝоОпɪɟɞɟлɟниɟɜɟличинкоэɮɮициɟнɬоɜɪɚɫпɪɟɞɟпɟкɬиноɜыɯɜɟщɟɫɬɜɫиɫɬɟмɚɯзɚɜиɫимоɫɬькиɫлоɬноɫɬиɫɪɟɞыпозɜолиɬɝнозиɪоɜɚɬьɜозможноɫɬьпɪоникноɜɟниячɟɪɟзɛиолоɝичɟɫкиɟɋлɟɞоɜɚɬɟльноможнопɪɟɞположиɬьчɬокɪыжоɜникɚлочнойɫɪɟɞɟкишɟчноɝоɫоɞɟɪжимоɝоɛɭɞɭɬɪɚɫпɪɟɞɟляɬьɫяɜɫɚɫыɜɚɬьɫяэкɫпɟɪимɟнɬɚльнойɝипɟɪɯолɟɫɬɟɪинɟмииоɬмɟчɚɟɬɫяɭɜɟличɟниɟконцɟнɬɪɚцииоɛщɟɝоɯолɟɫɬɟɪинɚɯолɟ 95,5% (p)() 110,6% (p)ɫооɬɜɟɬɫɬɜɟнноɬɚкжɟɫнижɟниɟɫоɞɟɪжɚнияɯолɟɫɬɟɪинɚиɫɫлɟɞɭɟмыɯпɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚоɬклонɟнноɝоɫпоɫоɛɫɬɜоɜɚлокоɪɪɟкцииноɪмɚлизɚконцɟнɬɪɚцииоɛщɟɝоɯолɟɫɬɟɪинɚɯолɟɫɬɟɪинɚɯолɟɫɬɟɪинɚ128ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияhydroxide, respectively. and the electrolyte were taken equally. The concentration nate was determined by the conductometric method us-nate was determined by the conductometric method us-Determination of antidislipemic activityAnimalsDetermination of the lipid-cholesterol pro? le of blood was performed on 30 male Wistar rats weighing 200-220 grams, divided into 3 equal groups (n = 10). The animal care and the experiments were in accordance with the rules of laboratory practice (GLP) for pre-clinical studies in the Russian Federation (GOST 3 51000.396 and 51000.4-96), tion for the Protection of Vertebrates (1997).Investigations of the effect of pectic substances iso- rst group of rats were intact animals (IA). In ower oil [8]. Hereby, the second group of rats were negative con-port. The third group of rats were injected per os with the per 100 g of the animal?s weight. le: total cholesterol (TC), tein cholesterol (LDLP cholesterol), high density lipo-protein cholesterol (HDLP cholesterol), and triglycerides (TG). The standard sets of reagents produced by Olvex Diagnosticum (total cholesterol: series 01042017, HDLP cholesterol: series 080022017, LDLP cholesterol: series 20022017, triglycerides: series 01122016, Sweden) were ational statistics methods using STATISTICA 6.0 applica-tion software package (StatSoft, USA). The obtained data iro-Wilk test. In the case of subordination of data to the pare the averages, otherwise further statistical processing RESULTS AND DISCUSSIONumn chromatography. ed water, ethyl alcohol of different concentrations and 95% ethyl alcohol. Two substances were obtained, one of which other had a dark brown color in UV light in the adsorp-Substance 4 having a dark-brown color in UV light in the The ethyl acetate fraction was concentrated to a small volume and mixed with anhydrous chloroform (1:5). The precipitate was transferred onto the column of polyamide, eluted with water-ethanol mixtures with a gradient increase in ethyl alcohol, and Substances 5 and 6 were obtained. The chloroform-ethyl acetate l-tate and transferred to a silica gel column. Elution with chloroform-ethanol mixtures with a gradient increase in ethyl alcohol yielded two fractions: one contained two ond contained four substances ? 9, 10, 11 and 12. Then umns. The elution was carried out with water-ethanol they are individual (Table 1) [9 ? 12].Table 1 ? Values of mobility coef cients of the obtained substancesValue of the mobility coef cient15% acetic acidBAW (4:1:5)0.36?0.020.42?0.030.44?0.030.59?0.040.64?0.020.32?0.020.26?0.020.78?0.040.83?0.040.52?0.030.77?0.030.80?0.030.43?0.020.73?0.020.64?0.020.07?0.010.89?0.030.90?0.02The IR spectrum of Substance 3, as well as the data of comparative chromatographic analysis, UV spectro-photometry with ionizing and complexing additives, are identical to those for Substance 11. This is also indicated by the absence of depression of the melting temperature in the sample of mixing. The similarity of some physical and 129Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134129 avonol. The structural analysis of these compounds was carried out by UV spec- spec-The UV spectra of aglycons (Substances 5 and 10) imately the same intensity. The obtained data indicate cant cant bathochro-all compounds at C7. The bathochromic shift of the avonoids having uence of without changing its intensity, which indicated a free hy-droxy group in C4? in these compounds [10].To clarify the position of the carbohydrate residue, UV spectroscopy of the aglycon in the presence of alumi-num chloride was used. When comparing the absorption spectra of the substances before and after hydrolysis, the cone attracts attention, which suggests that in Compounds trometer FCM 1201?. The carbonyl group of the unsub- avone was absorbed at 1650 cm-1. If a avone cy is reduced to 1619 cm-1. The introduction of a hy- avonoids slightly slightly As a result of alkaline cleavage of the investigated aglycones (2, 3, 5) with potassium hydroxide, protocat-echic acid and ? oroglucin were obtained, and as a result oroglucin were PC (the mobile phase was ? BAW (4: 1: 5), the developer (4: 1: 5), the developer As a result of complete acid hydrolysis of Substance 2, aglucone - quercetin and D-galactose were obtained. The hydrolyzate was treated with diethyl ether in a separatory funnel. The obtained extract was chroma-quercetin sample in the mobile phases of PC BAW (4: 1: 5) and 15% acetic acid. The hydrolyzate was neutral-ized with barium carbonate using a universal indicator, ltered, evaporated to a small residue and chroma-tographed in the mobile phase of PC BAW (4: 1: 5) using standard samples. After the development of anilinphthal-After the development of anilinphthal-The proton signals appear in NMR-1H spectrum (400MHz, DMSO-d6, δ, ppm, J / Hz) of Substance 10: 6.19 (1H, d, 1.96 Hz, H-6), 6 , 37 (1H, d, J = 1.96 Hz, H-8), 8.04 (2H, m, H-2 ?, 6?), 6.82 (2H, m, H-3 ?, 5 ?), 12.94 (1H, s, OH-5).NMR-13C spectrum of Substance 10 (101 MHz, DM-SO-d6): 154.21 (C-2); 135.98 (P-3); 180.52 (P-4); 104.77 9); 162.31 (P-10); 121.55 (C-1?); 130.15 (C-2 & apos;, 6 & apos;); 115.58 (C-3 & apos;, 5?); 157.73 (C-4?). rm our conclusions obtained on the basis of UV spectropho-The UV spectrum of Substance 7 is characterized by According to UV, IR spectroscopy and paper chromatog-raphy, it can be concluded that substance 7 is a coffee fee The results obtained indicate that Substance 5 is 3,5,7,3?,4?-pentaoxy? avone (quercetin), Substance 2 is avone (kaempferol), Substance 8 is kappepro-7 is coffee acid. cient cient in the n-octanol-puri ed water system is widely used in chemistry, medicine, and cient and the biological effect can be biological activity [17]. The results of determining the cient are presented in Tables 2, 3. cient was calculated ed water (C1) and n-octanol (C2). The concentration of the octanol solution was raised to the degree of the corresponding degree of association. The latter was cient and the degree of association.130ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияTable 2 ? Distribution Coef cient in systems water ? n-octanol, ed water-diethyl ether0.10.420.370.070.030.20.750.710.180.020.41.391.050.290.110.61.951.890.590.010.82.582.470.780.021.03.172.870.900.10Note: C is the initial concentration; R is the resistance of aqueous solutions of PS; C and Care the equilibrium concentra-Table 3 ? Effect of pH-medium on the distribution coef cient in the system ed water - n-octanol0.60.986.866.573.433.100.570.590.81.786.856.412.592.480.750.761.02.186.724.063.182.450.600.77are equilibrium concentrations of PC solutions after extractionof PS from European gooseberry, a better distribution from the aqueous layer to the organic layer occurs in n-octanol. Experimental data on the effect on the pH dis-for gooseberry goose is in the alkaline medium (Table 4).131Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134131Table 4 ? Values of the distribution coef cient in various media cientMedium11.59puri ed water ? n-octanol22.60puri ed water ? diethyl ether31.84puri ed water (pH = 2) ? n-octanol41.43puri ed water (pH = 8) ? n-octanolIt has been shown that the process of PC transition into the organic phase (n-octanol) is exothermic (H ) tion of the enthalpy component is greater than the entropy G This indicates the spontaneous distribution of polysaccharides to the organic layer.The effect of pectic substances isolated from European le of bloodlesterol, LDL cholesterol, HDL cholesterol and TG was Figure 2 ? Effect of PC on lipid-cholesterol blood pro leNote: tot. chol. ? total cholesterol, LDL chol. ? cholesterol of low density lipoproteins, HDL chol. ? cholesterol lipoprotein high density, TG-triglycerides, IN ? intact animals, NC ? negative control, PC ? pecti substances isolated from European goose-berry, leaves; cant (p)elative to the group of animals; * ? statistically signi cant (pt-Student criterion) relative to the group of NC animals tration of total cholesterol, LDL-C and TG by 95.5% (p)()10.6% (p)spectively. In the NC group of animals the content of HDL cholesterol, on the contrary, decreased by 86.5% , decreased by 86.5% Under the action of pectic substances obtained from European gooseberry leaves, the normalization of the le of blood was observed in rats, compared with that in the animals of the NC group (Figure 2), expressed in the concentration decrease of total cholesterol, LDL cholesterol and TG by 27.1% tively, and an increase in HDL cholesterol by 32.7% rm the bene cial effect of pectic substances on lipid-cholesterol metabo-fect of pectic substances on lipid-cholesterol metabo-glycerides, normalization of the ratio LDL cholesterol / HDL cholesterol with the input of the studied pectic sub-prevents their pro-atherogenic effects [20, 21]. Preparative column chromatog-raphy was used to extract quercetin, hyperoside, rutin, kaempferol, astragaline and caffeic acid from Europe-Grossularia reclinata (L.) Mill.,obtained by 40% Ethanol. Hyperoside, rutin, coffee 132ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияȻиɛлиоɝɪɚɮичɟɫкийПɟлиɜɚноɜɚɋɟлинɚȺнɞɪɟɟɜɚОɝɚнɟɫянПолиɮɟнольныйɫоɫɬɚɜкɪыжоɜниоɬклонɟнноɝошɟлкоɜицы // НɚɭчныɟɜɟɞомоɫɬиɋɟɪияМɟɞицинɚɎɚɪмɚцияНɚɭчȺльɛɟɪɬИзɛиɪɚɬɟльнɚяɬокɫичноɫɬьМоɫкɜɚОɜоɞоɜпɪɟɞɫɬɚɜлɟнияпɟкɬиноɜыɯɜɟщɟɫɬɜɚɯ // 3. КɪыжɚноɜɫкийȻоɝɞɚноɜичȻɟɫɟɞноɜɚИɜɚнɭшкоȽолоɜɚчɟɜɚȽиполипиɞɟмичɟпɪоɬиɜоɜоɫпɚлиɬɟльныɟэɮɮɟкɬыполиɫɚɯɚɪиɞоɜɛɭɪыɯɜоɞоɪоɫлɟйпɚциɟнɬоɜɎɭнɞɚмɟнɬɚльныɟиɫɫлɟɞоɜɚнияȻɭɛɟнчикоɜɚЛɟɜчɟнкоНɚɫɟɞкинИзɭчɟниɟполиɫɚɯɚɪиɞоɜɬɪɚɜыɯонɞɪиллыɫиɬникоɜиɞɌɪɚɞиционнɚямɟɞицинɚКочɟɬкоɜɛиолоɝичɟɫкиɚкɬиɜныɯɫоɟɞинɟнийПɟлиɜɚноɜɚȺнɞɪɟɟɜɚМыкоцОɝɚнɟɫянПолиɫɚɯɚɪиɞыкɪыжоɜникɚклонɟнноɝо (Grossularia reclinata (L.) Mill.) // ɊɚзɪɚɛоɬкɚиɫɫлɟɞоɜɚниɟмɚɪкɟɬинɝноɜойɮɚɪмɚцɟɜɬичɟɫкойпɪоɞɭкциинɚɭчПяɬиɝоɪɫкɊɭкоɜоɞɫɬɜоэкɫпɟɪимɟнɬɚльномɭɞоклиничɟɫкомɭизɭчɟниюɮɚɪмɚколоɝичɟɫкиɯɜɟщɟɫɬɜ / ПоɞɪɟɞɏɚɛɪиɟɜɚизɞИзɞɚɬɟльɫɬɜоМɟɞицинɚКочɟɬоɜɚɋɟмɟниɫɬɚяɊɟɜинɚОпɪɟɞɟлɟниɟɛиолоɝичɟɫкиɚкɬиɜныɯɫоɟɮɟнольнойполиɮɟнольнойпɪиɪоɞыоɛъɟкɬɚɯмɟɬоɞɚмиɯɪомɚɬоɝɪɚɮииɍɫпɟɯиИɜɚноɜɚɎɟɞоɪоɜɚИɜɚноɜɚɎɟɞоɪоɜОɫɬɪоɭɯоɜɚМɚлкоɜɎлɚɜоноиɞныɟɫоɟɞинɟниякоɪылиɫɬɜɟнницыɫиɛиɪɫкойлиɫɬɜɟнницыɝмɟлинɚ // ɪɚɫɬиɬɟльноɝоКɭличɟнкоȺнɞɪɟɟɜɚЛɭкɚшɭкМɚзɭɪинɚИɫɫлɟɞоɜɚниɟɯимичɟɫкоɝоɫоɫɬɚɜɚɚкɬиɜноɫɬипɟɪиɞɟɪмыклɭɛнɟйкɚɪɬоɮɟляɎɚɪмɚцияɮɚɪмɚколоɝия3. 4(11). DOI: 10.19163/2307-9266-2015-3-4(11)-4-7Ȼомɛɟлɚ T.B., ПɟɬɪичɟнкоКɪоɬкоɜɚ O.A. ɎɟнольныɟɫоɟɞинɟниянɟкоɬоɪыɯɪоɞɚОчɚнкɚɮлоɪыПɟɪмɫкоɝоɪɚɫɬиɬɟльноɝо. 2011. ȻɚнɞюкоɜɚШинкɚɪɟнкоКɚзɚкоɜМɟɬоɞыиɫɫлɟɞоɜɚнияпɪиɪоɞныɯɮлɚɜоноиɞоɜɝоɪɫкИзɞȻɚльнɟолКоɪɭлькинȺɛилоɜМɭзычкинɚɌолɫɬикоɜПɪиɪоɞныɟɮлɚɜоноиɞыНоɜоɫиɛиɪɫкȽɟоȽолоɜкинɊɭɞɟнɫкɚяɌɪоɮимоɜɚȻиолоɝичɟɫкиɚкɬиɜныɟɜɟщɟɫɬɜɚɬɟльноɝопɪоиɫɯожɞɟнияНɚɭкɚЧɟɪɜоннɚяОɝɚнɟɫянȺнɞɪɟɟɜɚɋɟнчɟнкоȻоɪоɜɫкийɋпоɫоɛполɭчɟнияɫɬɚнɞɚɪɬɚпɚɬɭлɟɬинɚɫоцɜɟɬийɛɚɪɯɚɬцɟɜɪɚɫпɪоɫɬɟɪɬыɯ (Tagetes patula L.) // Зɞоɪоɜьɟоɛɪɚзоɜɚниɟ XXI ɜɟкɟКолɛинɋоломиноɜɚɌюɪинɚКɪылоɜɚɊɚɫчɟɬɜɟɪиɮикɚциякоэɮɮициɟнɬоɜокɬɚнолɜоɞɚɝɟɪɛициɞоɜɯимичɟɫкийжɭɪнɚлKu C.S., Kim B, Pham T.X., Yang Y., Weller C.L., Carr T.P., Park Y.K., Lee J.Y. Hypolipidemic Effect of a Blue-Green Alga (Nostoc commune) Is Attributed to Its Nonlipid Fraction by Decreasing Intestinal Cholesterol Absorption in C57BL/6J Mice // Journal of Medicinal Food. 2015. 18(11). P. 1214 ? 1222. DOI: 10.1089/Jiang T., Gao X., Wu C., Tian F., Lei Q., Bi J., Xie B., Wang H.Y., Chen S., Wang X. Apple-Derived Pectin Modulates Gut Microbiota, Improves Gut Barrier Function, and Attenuates Metabolic Endotoxemia in Rats with Diet-Induced Obesity Mice // Nutrients. 2016. 8(3). P. 126.Jesch E.D., Carr T.P. Food Ingredients That Inhibit Cholesterol Absorption // Preventive Nutrition and Food Science. 2017. 22(2). P. 67-80. DOI: 10.3746/pnf.2017.22.2.67Jakobsdottir G., Xu J., Molin G., Ahrne S., Numan M. High-Fat Diet Reduces the Formation of Butyrate, but ammation, Liver Fat and Cholesterol in Rats, while Dietary Fibre Counteracts These Effects // . 2013. No. 8(11): e80476. rst isolated from European gooseberry cients values biological membranes. 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DOI: 10.1371/journal.pone.008047134ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияКонɮликɬинɬɟɪɟɫоɜȺɜɬоɪызɚяɜляюɬоɬɫɭɬɫɬɜииконɮликɬɚинɬɟɪɟɫоɜȺɞжиɚɯмɟɬоɜɚЛɟонɬьɟɜнɚ ? ɞɚɜɚɬɟлькɚɮɟɞɪыоɪɝɚничɟɫкойПяɬиɝоɪɫкомɟɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ɚлɚȼолɝȽМɍМинзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯинɬɟɪɟɫоɜпɪиɪоɞныɯɫоɟɞинɟнийПозɞнякоɜ ? пɪɟпоɞɚɜɚɬɟлькɚɮɟɞɪыɮɚɪмɚколоɝиикɭɪɫомклиничɟɫкойɮɚɪмɚколоɝииПяɬиɝоɪɫкоɝомɟɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ɮилиɚлɚȼолɝȽМɍМинзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯинɬɟɪɟɫоɜɜɟщɟɫɬɜлɚɞɚющиɯэнɞоɬɟлиопɪоɬɟкɬиɜнойɚкɬиɜноɫɬьюE-mail: pozdniackow.dmitry@yandex.ruЧɟɪɜоннɚяНɚɞɟжɞɚМиɯɚйлоɜнɚ ? кɚɮɟɞɪыоɪɝɚничɟɫкойПяɬиɝоɪɫкоɝоɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ɮилиɚлɚȼолɝȽМɍМинзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯɬɟɪɟɫоɜпɪиɪоɞныɯɫоɟɞинɟнийМыкоцПɟɬɪоɜнɚ ? кɚнɞиɞɚɬɮɚɪмɚцɟɜɬичɟɫкиɯнɚɭкɞоцɟнɬкɚɮɟɞɪынɟоɪɝɚничɟɫкойɮизичɟɫкойколлоиɞнойПяɬиɝоɪɫкоɝоɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ɮилиɚлɚȼолɝȽМɍМинзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯɪɟɫоɜɮизикоɯимичɟɫкиɟɫɜойɫɬɜɚпɪиɪоɞныɯɫоɟȼоɪонкоɜȼлɚɞиɫлɚɜоɜич ? ɞокɬоɪнɚɭкзɚɜɟɞɭющийкɚɮɟɞɪойɮɚɪмɚколоɝиикɭɪɫомклиничɟɫкойɮɚɪмɚколоɝииПяɬиɝоɪɫкоɝомɟɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ȼолɝȽМɍМинзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯинɬɟɪɟɫоɜɜɟщɟɫɬɜоɛлɚɞɚющиɯэнɞоɬɟлиопɪоɬɟкɬиɜнойɚкɬиɜноɫɬьюɪɚзɪɚɛоɬкɚɮɚɪмɚколоɝичɟɫкойкоɪɪɟкцииɫоɫɬоянийɜозникɚюиɫпыɬыɜɚющиɯпоɫɬоянноɟэкɫɬɪɟмɚльɮизичɟɫкоɟпɫиɯоэмоционɚльноɟнɚпɪяжɟниɟɬомчиɫлɟɫпоɪɬɟɜыɫокиɯɞоɫɬижɟнийa.v.voronkov@pmedpharm.ruОɝɚнɟɫянЭɞɭɚɪɞɌоникоɜич ? ɞокɬоɪɮɚɪмɚцɟɜɬичɟɫкиɯнɚɭкпɪоɮɟɫɫоɪзɚɜɟɞɭющийкɚɮɟɞɪойоɪɝɚничɟɫкойПяɬиɝоɪɫкоɝомɟɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ɮилиɚлɚȼолɝȽМɍзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯинɬɟɪɟɫоɜпɪиɪоɞныɯɫоɟɞинɟнийɫинɬɟɬичɟɫкиɯɚнɚлоɝоɜиɫɫлɟɞоɜɚниɟпɪомышлɟнныɯоɬɯоɞоɜпищɟɜоɝоɮɚɪмɚцɟɜɬичɟɫкоɝопɪоизɜоɞɫɬɜɚкɚкɞополниɬɟльиɫɬочникɚполɭчɟниялɟчɟɛнопɪоɮилɚкɬичɟɫɪɟɞɫɬɜмɟɞицинɫкɚя. E-mail: edwardov@ПоɫɬɭпилɚɪɟɞɚкциюОɬпɪɚɜлɟнɚɞоɪɚɛоɬкɭПɪиняɬɚпɟчɚɬи ict of interest ict of interest.? Teacher of the Department of Organic Chemistry, Pyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State University. Research interests: chemistry Teacher of the De-Pharmacology, Pyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State Univer-sity. Research interests: search for substances of endo-thelioprotective activity. E-mail: pozdniackow.dmitry@ - postgradu-ate student of the Department of Organic Chemistry, Py-of Volgograd Medical State University. Research inter-? PhD (Pharmacy), Asso-ciate Professor of the Department of Inorganic, Physical and Colloid Chemistry, Pyatigorsk Medical and Pharma-ceutical Institute, a branch of Volgograd Medical State University. Research interests: physical and chemical properties of natural compounds. E-mail: 58041@mail.Voronkov Andrey Vladislavovich course of clinical pharmacology,Pharmaceutical Institute, a branch of Volgograd Medi-cal State University. Research interests: search for sub-stances with endothelioprotective activity; development of ways of pharmacological correction of conditions arising in individuals experiencing constant extreme physical and psychoemotional stress, including sports innovative approaches in the sphere of postgraduate ed-ucation specialists. E-mail: prohor.77@mail.ruOganesyan Eduard Tonikovich Professor, Head of the Department of Organic Chem-Volgograd Medical State University.Research interests: alogues; research of industrial wastes of food and phar-maceutical production as an additional source of medi-cal and prophylactic drugs, medical chemistry. E-mail: edwardov@mail.ru121Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134121ɍȾК 582.711.31:547.56/.458.88:615.076.9 ˇʫʻОЛːʻˏʫʿʫК˃ʰʻОʦˏʫʦʫˍʫˁ˃ʦʤКˀˏʮОʦʻʰКʤО˃КЛОʻʫʻʻОʧОGROSSULARIA RECLINATA MILL.ȺɞжиɚɯмɟɬоɜɚПозɞнякоɜЧɟɪɜоннɚяМыкоцȼоɪонкоɜОɝɚнɟɫянмɟɞикоɮɚɪмɚцɟɜɬичɟɫкийинɫɬиɬɭɬɎȽȻОɍȼолɝоɝɪɚɞɫкийɝоɫɭɞɚɪɫɬɜɟнныймɟɞицинɫкийɭниɜɟɪɫиɬɟɬМинзɞɪɚɜɚɊоɫɫииɊоɫɫия, 11кɪыжоɜникɚоɬклонɟнноɝооɛнɚɪɭжɟно 23 ɫоɟɞинɟниякоɬоɪыɯɫɬɚнɞɚɪɬоɜиɞɟнɬиɮициɪоɜɚноɬолько 13 ɜɟщɟɫɬɜпокɚзɚɬɟлɟйɛиоɞоɫɬɭпноɫɬияɜляɟɬɫяэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияиɫɫлɟɞɭɟмыɯɜɟщɟɫɬɜмɟжɞɭɬɚкжɟɪɚзличныɯɛиолоɝичɟɫкиɯжиɞкоɫɬяɯПɟкɬиноɜыɟɜɟщɟɫɬɜɚоɛлɚɞɚюɬпɪоɮилɚкɬичɟɫкимɞɟйɫɬɜиɟмɬиɜоɜоɫпɚлиɬɟльнымɚнɬиɛɚкɬɟɪиɚльнымпɪоɬиɜоɫклɟɪоɬичɟɫкимɚнɬиɚллɟɪɝɟнɬнымЦɟльɪɚɛоɬыɜыɞɟлɟниɟиɞɟнɬиɮикɚцияɮɟнольныɯɫоɟɞинɟнийиɫɫлɟɞоɜɚниɟкоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияпиɞɟмичɟɫкойɚкɬиɜноɫɬипɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚоɬклонɟнноɝо (Grossularia reclinata (L.) МɚɬɟɪиɚлымɟɬоɞыОɛɟзжиɪɟнноɟкɪыжоɜникɚоɬклонɟнноɝоэкɫɬɪɚɝиɪоɜɚли 40% ɫпиɪɬомэɬилоɜымнɚɝɪɟɜɚнииɬɟчɟниɟ 60 ОɛъɟɞинɟнныɟизɜлɟчɟнияɞɟлиɬɟльнойɜоɪонкɟпоɫлɟɞоɜɚɬɟльнооɛɪɚɛɚɬыɜɚлиɯлоɪоɮоɪмомɞиэɬилоɜымэɮиɪомэɬилɚцɟɬɚɬомɛɭɬɚноломȼыɞɟлɟниɟпɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚоɬклонɟнноɝопɪоɜоɞилимɟɬоɞикɟКочɟɬкоɜɚ M. Sinner. опɪɟɞɟлɟниикоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияоɪɝɚничɟɫкиɯɪɚɫɬɜоɪиɬɟлɟйиɫпользоɜɚныɞиэɬилоɜыйэɮиɪокɬɚнолкоɬоɪыɯизɭчɚɟмыɟоɛъɟкɬыɯоɪошоɪɚɫɬɜоɪимыПɪоцɟɞɭɪыɜɜɟɞɟнияɪɚɫɬɜоɪоɜиɫɫлɟɞɭɟмыɯпɟкɬиноɜыɯɜɟщɟɫɬɜɪɚɫɬɜоɪɚɯолɟɫɬɟɪинɚɪɚзɞɟлɟны 2-чɚɫоɜыминɬɟɪɜɚломиɫɬɟчɟнии 14 жиɜоɬныɯпɪоизɜоɞилизɚɛоɪɜɟнознойпоɫлɟɞɭющимполɭчɟниɫыɜоɪоɬкиопɪɟɞɟлɟниɟмпɚɪɚмɟɬɪоɜɯɚɪɚкɬɟɪизɭющиɯɫоɫɬояниɟɯолɟɫɬɟɪиноɜоɝоɊɟзɭльɬɚɬыоɛɫɭжɞɟниɟɪɟзɭльɬɚɬɟиɫɫлɟɞоɜɚнияɮɟнольныɯɫоɟɞинɟнийкɪыжоɜниоɬклонɟнноɝоɜыɞɟлɟныɝипɟɪозиɞɪɭɬинкоɮɟйнɚякиɫлоɬɚПолɭчɟнныɟɫɜиɞɟɬɟльɫɬɜɭюɬɜоɞныɯɪɚɫɬɜоɪоɜпɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚлɭчшɟɟɪɚɫпɪɟɞɟлɟниɟɜоɞноɝоɫлояоɪɝɚничɟɫкийпɪоиɫɯоɞиɬокɬɚнолпɟкɬиноɜыɯɜɟщɟɫɬɜполɭчɟнныɯкɪыжоɜникɚжиɜоɬнымиконɬɪоляɝɪɭппынɚɛлюɞɚлɚɫьноɪмɚлизɚцияɯолɟɫɬɟɪиноɜоɝоЗɚключɟниɟМɟɬоɞомколоночнойɯɪомɚɬоɝɪɚɮииизɜлɟчɟниикɪыжоɜникɚоɬклонɟнноɝооɛнɚɪɭжɟныкɜɟɪцɟɬинɝипɟɪозиɞɪɭɬинкɟмпɮɟɪолкоɮɟйнɚякиɫлоɬɚМожнопɪɟɞположиɬьпɟкɬиноɜыɟɜɟщɟɫɬɜɚжоɜникɚщɟлочнойɫɪɟɞɟкишɟчноɝоɫоɞɟɪжимоɝоɛɭɞɭɬлɭчшɟɪɚɫпɪɟɞɟляɬьɫяɜɫɚɫыɜɚɬьɫяиɫɫлɟɞɭɟмыɯпɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚɫпоɫоɛɫɬɜоɜɚлокоɪɪɟкцииɞиɫлипиɞɟмииКлючɟɜыɟɫлоɜɚкɪыжоɜникɚоɬклонɟнноɝоɮлɚɜоноиɞыɮɟнолокиɫлоɬыполиɫɚɯɚɪиɞыкоэɮɪɚɫпɪɟɞɟлɟнияɞиɫлипиɞɟмияциɬиɪоɜɚнияȺɞжиɚɯмɟɬоɜɚПозɞнякоɜЧɟɪɜоннɚяМыкоцȼоɪонкоɜОɝɚнɟɫянɎȿНОЛЬНЫȿȼȿЩȿɋɌȼȺКɊЫЖОȼНИКȺ (GROSSULARIA RECLINATA (L.) MILL.). ɎɚɪмɚцияɮɚɪмɚколоɝияȺɞжиɚɯмɟɬоɜɚПозɞнякоɜЧɟɪɜоннɚяМыкоцȼоɪонкоɜОɝɚнɟɫянFor citation:Myikots L.P.,Voronkov A.V., Oganesyan E.T. PHENOLIC COMPOUNDS AND PECTIC SUBSTANCES OF EUROPEAN GOOSEBERRY LEAVES (GROSSULARIA RECLINATA (L.) MILL.).Pharmacy & Pharmacology.122ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝиякɪыжоɜникɚоɬклонɟнноɝомɟɬоɞомɜыɫокоэɮɮɟкɬиɜнойжиɞкоɫɬноймɚɬоɝɪɚɮииɪɭжɟно 23 ɫоɟɞинɟниякоɬоɪыɯнɚличиюɫɬɚнɞɚɪɬоɜиɞɟнɬиɮициɪоɜɚноɬолько 13 ɜɟщɟɫɬɜпɪɟɞɫɬɚɜлɟныɮлɚɜоноиɞɚмикɭмɚɪиɮɟнолкɚɪɛоноɜымикиɫлоɬɚми 13 Ⱦля ɛолɟɟпоɞɪоɛнойинɮоɪмɚцииполиɮɟнольномɫоɫɬɚɜɟизɜлɟчɟниякɪыжоɜникɚоɬклонɟнноɝоGrossularia reclinata (L.) Mill.лɭчɟнноɝоэкɫɬɪɚкциɟй 40% ɫпиɪɬомпɪоɜɟлиɟɝоɮɪɚкциониɪоɜɚниɟоɛɪɚɛоɬкойɪɚɫɬɜоɪиɬɟлямиɫочɟɬɚниипɪɟпɚɪɚɬиɜнойколоночнойɯɪомɚɬоɝɪɚɮиɟйОɞнимпокɚзɚɬɟлɟйɛиоɞоɫɬɭпноɫɬипɪɟпɚɪɚɬоɜяɜляɟɬɫякоэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияиɫɫлɟɞɭɟмыɯɜɟщɟɫɬɜɬɚкжɟɛиолоɝичɟɫкиɯжиɞкоɫɬяɯ [2].ПɟкɬиноɜыɟɜɟщɟɫɬɜɚоɛлɚɞɚюɬɬичɟɫкимпɪоɬиɜоɜоɫпɚлиɬɟльнымɛɚкɬɟɪиɚльнымпɪоɬиɜоɫклɟɪоɬичɟɫкимɚнɬиɚллɟɪPHENOLIC COMPOUNDS AND PECTIC SUBSTANCES OF EUROPEAN GOOSEBERRY LEAVES GROSSULARIA RECLINATA MILL.S.L. Adjiahmetova, D.I. Pozdnyakov, N.M. Chervonnaya, L.P. Myikots, A.V. Voronkov, E.T. OganesyanPyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State University 11, Kalinin ave., Pyatigorsk, Russia, 357532In the leaves of European gooseberry 23 compounds have been found, and only 13 of them have been identi ed by the presence of standards. One of the indicators of the bioavailability of drugs is the distribution coef cient of the sub-stances under investigation between the hydrophilic and lipophilic phases, as well as in various biological uids. Pec-tic substances have a preventive effect: anti-in ammatory, antibacterial, anti-sclerotic, anti-allergic. isolation, identi cation of phenolic compounds, investigation of the distribution coef cient and the antidis-lipidemic activity of the pectic substances in European gooseberry leaves (Grossularia reclinata (L.) Mill.). Degreased raw materials (leaves of European gooseberry) were extracted three times with 40% ethanol by heating for 60 minutes. In the separatory funnel the combined extracts were subsequently treated with chloroform, diethyl ether, ethyl acetate and n-butanol. Isolation of pectin substances from the leaves of European gooseberry was carried out according to N.K. Kochetkov and M. Sinner?s method. When determining the distribution coef cient from organic solvents, diethyl ether and n-octanol, in which the objects under study are readily soluble, have been used. The procedures of the solutions input of the investigated pectic substances and cholesterol solution were separated by a 2-hour interval. After 14 days, venous blood was collected from the animals, followed by the production of se-rum and determination of the parameters characterizing the state of the lipid-cholesterol blood pro As a result of the study of phenolic compounds of European gooseberry leaves, hyperoside, rutin, caffeic rst time. The data obtained show that for aqueous solutions of pectic substances of European gooseberry, the best distribution from the aqueous layer to the organic layer occurs in n-octanol. When using the pectic substances obtained from European gooseberry leaves, the normalization of the lipid-cholesterol pro- le in blood was observed in rats in comparison with the animals of the negative control group. By the method of preparative column chromatography, quercetin, hyperoside, rutin, kaempferol, astragaline, and caffeic acid were detected in European gooseberry leaves. It can be assumed that the pectic substances from gooseberry leaves will be better distributed and absorbed in the alkaline environment of intestinal contents. The use of the studied pectic substances from gooseberry leaves promoted the correction of dyslipidemia. European gooseberry leaves, avonoids, phenolic acids, polysaccharides, distribution coefɝɟнɬнымОɛлɚɞɚюɬɫɜойɫɬɜомкоɪɪɟкциилипиɞноɝооɛмɟнɚЦȿЛЬɊȺȻОɌЫиɞɟнɬиɮикɚцияɮɟнольныɯɫоɟɞинɟнийиɫɫлɟɞоɜɚниɟкоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияɚнɬиɞиɫлипиɞɟмичɟɫкойɚкɬиɜнопɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚоɬклонɟнноɝоGrossularia reclinata (L.) MillМȺɌȿɊИȺЛЫМȿɌОȾЫПолɭчɟниɟизɜлɟчɟнияɜыɞɟлɟниɟинɞиɜиɞɭɫоɟɞинɟнийкɚчɟɫɬɜɟиɫɫлɟɞɭɟмоɝоиɫпользоɜɚкɪыжоɜникɚоɬклонɟнноɝоиюлɟ 2015?2017 ɮɚзɭцɜɟɬɟнияɫоɪɬМоɫкоɜɫкийКɚɜкɚзɫкиɟМинɟɪɚльныɟȼоɞыɋɬɚɜɪопольОɛɟзжиɪɟнноɟкɪыжоɜникɚоɬклонɟнноɝоɬɪижɞыэкɫɬɪɚɝиɪоɜɚли 40% ɫпиɪɬомнɚɝɪɟɜɚнииɬɟчɟниɟ 60 Полɭчɟнноɟизɜлɟчɟниɟɫɝɭщɚлиоɛъɟмɚ 100 поɫлɟэɬоɝо123Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134123ɜоɪонкɟпоɫлɟɞоɜɚɬɟльнооɛɪɚɛɚɬыɜɚлиɯлоɪоɮоɪмомэɮиɪомэɬилɚцɟɬɚɬомɛɭɬɚноломПолɭчɟнныɟпоɫлɟɭɞɚлɟнияɪɚɫɬɜоɪипɟɪɟноɫилиɯɪомɚɬоɝɪɚɮичɟɫкиɟколонкизɚполнɟнныɟɫиликɚɝɟлɟммɚɪки 60 лиɚмиɞоммɚɪкиPolyamide WoelmЭлюɟнɬомɫлɭжилɚɜоɞɚочищɟннɚяɫиликɚɝɟляɯлоɪоɮоɪмПɪоцɟɫɫэлюиɪоɜɚнияконɬɪолиɪоɜɚлимɟɬоɞɚмиɬонкоɫлойнойɯɪомɚɬоɝɪɚɮииɛɭмɚжнойɯɪомɚɬоɝɪɚɮииMUNKTELL FN 7). кɚчɟɫɬɜɟпоɞɜижнойпользоɜɚлиɛɭɬɚнол : киɫлоɬɚɭкɫɭɫнɚя : ɜоɞɚȻɍȼкиɫлоɬɚɭкɫɭɫнɚяэɬилɚцɟɬɚɬ ? киɫлоɬɚɭкɫɭɫнɚяɜоɞɚȼыɞɟлɟниɟпɟкɬиноɜыɯɜɟщɟɫɬɜопɪɟɞɟлɟниɟкоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияпɟкɬиноɜыɯɜɟщɟɫɬɜжоɜникɚоɬклонɟнноɝопɪоɜоɞилимɟɬоɞикɟКочɟɬкоɜɚ M. Sinner [6]. ПоɫлɟɝиɞɪолизɚпɟкɬиноɜыɯɜɟщɟɫɬɜɪɭжɟныɝлюкозɚкɫиɝɚлɚкɬɭɪоноɜɚякиɫлоɬɚȽɪɚɜимɟɬɪичɟɫкийɚнɚлизɭкɚзыɜɚɟɬпɪɟоɛлɚɞɚниɟ (6,4%). Поэɬомɭɞɚльнɟйшиɟиɫɫлɟɞоɜɚнияпɪоɜоɞилиэɬой (6,4%). Пɪи опɪɟɞɟлɟниикоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияоɪɝɚничɟɫкиɯɪɚɫɬɜоɪиɬɟлɟйиɫпользоɜɚныэɮиɪокɬɚнолкоɬоɪыɯоɛъɟкɬыɯоɪошоɪɚɫɬɜоɪимыЭкɫɬɪɚкцияпɪоɜоɞилɚɫькɚкɜоɞныɯɪɚɫɬɜоɪоɜ (0,1-1%), ɬɚкɪɚɫɬɜоɪоɜɞоɜɟɞɟнныɯ pH=2 pH=8 ɪɚɫɬɜоɪоɜкиɫлоɬыɯлоɪиɫɬоɜоɞоɪоɞнойɝиɞɪокɫиɞɚнɚɬɪияɫооɬɜɟɬɫɬɜɟнноэкɫɬɪɚкцииоɛъɟмыиɫɫлɟɞɭɟмыɯɪɚɫɬɜоɪоɜэлɟкɬɪолиɬɚоɞинɚкоɜоКонцɟнɬɪɚцияɜоɞныɯɪɚɫɬɜоɪоɜнɚчɚльнɚяɪɚɮинɚɬɟопɪɟɞɟлялɚɫьконɞɭкɬомɟɬɪичɟɫкиммɟɬоɞомиɫпользоɜɚниɟмкɚлиɛɪоɜочноɝɪɚɮикɚзɚɜиɫимоɫɬи pH=8 ОпɪɟɞɟлɟниɟɚнɬиɞиɫлипиɞɟмичɟɫкойɚкɬиɜЖиɜоɬныɟОпɪɟɞɟлɟниɟɯолɟɫɬɟɪиноɜоɝоɜыполняли 30 кɪыɫɚɯ ? ɫɚмцɚɯWistarмɚɫɫой 200?220 ɪɚзɞɟлɟнныɯ 3 ɝɪɭппы (n=10). ɋоɞɟɪжɚниɟжиɜоɬныɯпɪоɜɟɞɟниɟэкɫпɟɪимɟнɬоɜɫооɬɜɟɬɫɬɜоɜɚлоɬоɪнойпɪɚкɬики (GLP) пɪоɜɟɞɟниииɫɫлɟɞоɜɚнийɊоɫɫийɫкойɎɟɞɟɪɚцииȽОɋɌ 51000.4-96), мɟжɞɭнɚɪоɞнымɪɟкомɟнȿɜɪопɟйɫкойконɜɟнциипозɜоночжиɜоɬныɯИɫɫлɟɞоɜɚнияпɟкɬиноɜыɯɜɟщɟɫɬɜɜыɞɟлɟнныɯкɪыжоɜникɚоɬклонɟннокоɪɪɟкциюɞиɫлипиɞɟмииПɟɪɜɚяɝɪɭппɚ ? инɬɚкɬныɟжиɜоɬныɟоɫɬɚɜшиɯɫямоɞɟлиɪоɜɚлиɝипɟɪɯолɟɫɬɟɪинɟкɭɪɫоɜоɝо (14 пɟɪоɪɚльноɝоɜɜɟɞɟ 3% ɪɚɫɬɜоɪɚɯолɟɫɬɟɪинɚɫɟɪияпоɞɫолнɟчноммɚɫлɟ [8]. эɬомɜɬоɪɭюɝɪɭппɭɫоɫɬɚɜилижиɜоɬныɟнɟɝɚɬиɜноɝоконɬɪоляполɭчɚɜшиɟɮɚɪмɚколоɝичɟɫкɭюпоɞɞɟɪжкɭɌɪɟɬьɟйɝɪɭппɟɜɜоɞилииɫɫлɟɞɭпɟкɬиноɜыɟɜɟщɟɫɬɜɚкɪыжоɜникɚоɬклонɟнноɝоколичɟɫɬɜɟ 1 ɪɚɫɬɜоɪɚɜɟɫɚжиɜоɬноɝоПɪоцɟɞɭɪыɜɜɟɞɟнияɪɚɫɬɜоɪоɜиɫɫлɟɞɭɟмыɯɜɟщɟɫɬɜɪɚɫɬɜоɪɚɯолɟɫɬɟɪинɚɪɚзɞɟ 2-инɬɟɪɜɚломиɫɬɟчɟнии 14 жиɜоɬныɯпɪоизɜоɞилиɜɟныпоɫлɟɞɭющимполɭчɟниɟмɫыɜоɪоɬкиопɪɟɞɟлɟнипɚɪɚмɟɬɪоɜɯɚɪɚкɬɟɪизɭющиɯɫоɫɬояниɟɯолɟɫɬɟɪиноɜоɝооɛщɟɝоɯолɟɫɬɟɯолɯолɟɫɬɟɪинɚлипопɪоɬɟиɞоɜнизкойплоɬноɫɬиɯолɯолɟɫɬɟɪинɚлипопɪоɬɟиɞоɜɜыɫокойплоɬноɫɬиɯолɬɪиɝлицɟɪиɞоɜɪɚɛоɬɟиɫпользоɜɚлиɫьɫɬɚнɞɚɪɬныɟɪɟɚкɬиɜоɜпɪоизɜоɞɫɬɜɚОльɜɟкɫȾиɚɝноɫɬикɭмɯолɟɫɬɟɪинɫɟɪия 01042017, ɯолɟɫɬɟɪинɫɟɪия 080022017, ɯолɟɫɬɟɪинɫɟɪия 20022017, ɬɪиɝлицɟɪиɞыɫɟɪия 01122016, ШɜɟцияПɪоɛопоɞɝоɬоɜкɚɞɚльнɟйшийɯоɞɚнɚлизɚɫооɬɜɟɬɫɬɜоɜɚлиинɫɬɪɭкциикɚжɞомɭнɚɛоɪɭɋɬɚɬиɫɬичɟɫкɚяоɛɪɚɛоɬкɚɊɟзɭльɬɚɬыэкɫпɟɪимɟнɬɚоɛɪɚɛɚɬыɜɚлимɟɬоɞɚɜɚɪиɚционнойɫɬɚɬиɫɬикииɫпользоɜɚниɟмпɚкɟ STATISTICA 6.0 (ПолɭчɟнныɟпоɞɜɟɪɝɚлиɬɟɫɬɭмɚльноɫɬьɪɚɫпɪɟɞɟлɟнияиɫпользоɜɚниɟмШɚпиɪоɍилкɚпоɞчинɟниязɚконɭноɪмɚльноɝоɪɚɫпɪɟɞɟлɟнияɫɪɟɞниɯ t-ɋɬьюɞɟнɬɚпɪоɬиɜномɞɚльнɟйшɭюɫɬɚɬиɫɬичɟɫкɭюоɛɪɚɛоɬкɭпɪоизɜоɞилипомощьюɍиɬниɊȿЗɍЛЬɌȺɌЫОȻɋɍЖȾȿНИȿȼыɞɟлɟниɟинɞиɜиɞɭɚльныɯɜɟщɟɫɬɜмɟɬоɞомколоночнойɯɪомɚɬоɝɪɚɮииɏлоɪоɮоɪмнɭюпɟɪɟноɫиликолонкɭполиɚмиɞомэлюиɪоɜɚлипоɫлɟɞоɜɚɬɟльноɜоɞойочищɟннойɫпиɪɬомконцɟнɬɪɚцииɫпиɪɬом 95%. Полɭчɟныɞɜɚɜɟщɟɫɬɜɚоɞинкоɬоɪыɯɯɚɪɚкɬɟɪизɭɟɬɫяɝолɭɛымɫɜɟчɟниɟмɜɟщɟɫɬɜоɜɬоɪоɟɛɭɪɭюокɪɚɫкɭзоныɫɜɟɬɟɜɟщɟɫɬɜоэɮиɪколонкɟɫиликɚɝɟлɟмɞɜɚɜɟщɟɫɬɜɚ ? ɜɟщɟɫɬɜоɜɟщɟɫɬɜоɫɜɟɬɟзонɭɛɭɪоɝоцɜɟɬɚЭɬилɚцɟɬɚɬнɭюɫɝɭщɚлинɟɛольшооɛъɟмɚɫмɟшиɜɚлиɛɟзɜоɞнымɯлоɪоɮоɪмомоɫɚɞокпɟɪɟноɫиликолонкɭполиɚмиɞомэлюиɪоɜɚлиɜоɞноэɬɚнольнымиɭɜɟличɟниɟмɫпиɪɬɚэɬилоɜоɝополɭчилиɜɟщɟɫɬɜɚɏлоɪоɮоɪмноɬɚɬныйɮильɬɪɚɬпоɫлɟоɬɞɟлɟнияоɫɚɞкɚɫɝɭщɚлипɟɪɟноɫиликолонкɭɫиликɚɝɟлɟмЭлюиɪоɜɚниɟмɯлоɪоɮоɪмноэɬɚнольнымиɫмɟɫямиɭɜɟличɟниɟмɫпиɪɬɚэɬилоɜоɝополɭчɟныɞɜɟоɞнойɫоɞɟɪжɚɬɫяɞɜɚɫоɟɞинɟнияполиɮɟнольнойпɪиɪоɞыɜɟщɟɫɬɜɚɜɬоɪой124ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияɜɟщɟɫɬɜɚ ? ɜɟщɟɫɬɜɚ11 ȾɚлɟɟоɛɟпоɞɜɟɪɝлипоɜɬоɪномɭɪɚзɞɟлɟниюколонкɚɯполиɚмиɞомЭлюиɪоɜɚниɟоɫɭщɟɫɬɜлялиɜоɞноэɬɚнольнымиɫмɟɫямиɏɪомɚɬоɝɪɚɮичɟɫкийɚнɚлизмɚлыйинɬɟɪɜɚлɬɟмпɟɪɚɬɭɪплɚɜлɟнияɜɟщɟɫɬɜɬɟльɫɬɜɭɟɬɬомчɬояɜляюɬɫяинɞиɜиɞɭɚльныɬɚɛл ɌɚɛлицɚЗнɚчɟниякоэɮɮициɟнɬоɜпоɞɜижноɫɬиполɭчɟнныɯɜɟщɟɫɬɜЗнɚчɟниɟкоэɮɮициɟнɬɚпоɞɜижноɫɬиНомɟɪполɭчɟнноɝоɜɟщɟɫɬɜɚкиɫлоɬɚɭкɫɭɫнɚяȻɍȼэɬилɚцɟɬɚɬкиɫлоɬɚɭкɫɭɫнɚяɜоɞɚ0,36?0,020,42?0,030,44?0,030,59?0,040,64?0,020,32?0,020,26?0,020,78?0,040,83?0,0450,52?0,030,77?0,030,80?0,0370,43?0,020,73?0,020,64?0,0280,07?0,010,89?0,030,90?0,0210ɫпɟкɬɪɜɟщɟɫɬɜɚɬɚкжɟɬɟльноɝоɯɪомɚɬоɝɪɚɮичɟɫкоɝоɚнɚлизɚɬɪоɮоɬомɟɬɪииионизиɪɭющимикомплɟкɫооɛɪɚзɭющимиɞоɛɚɜкɚмиɚнɚлоɝичнымпɚɪɚмɟɬɪɚмɜɟщɟɫɬɜɚэɬомɫɜиɞɟɬɟльɫɬɜɭɬɚкжɟоɬɫɭɬɫɬɜиɟɞɟпɪɟɫɫииɬɟмпɟɪɚɬɭɪыплɚɜлɟпɪоɛɟȼыяɜлɟнɚɫɯожɟɫɬьнɟкоɬоɪыɯɮизикоɯимичɟɫкиɯпɚɪɚмɟɬɪоɜɜɟщɟɫɬɜȼɟщɟɫɬɜɚпɪиоɛɪɟɬɚюɬкɪɚɫноɟшиɜɚниɟциɚниɞиноɜойчɬоɞɟɬɟльɫɬɜɭɟɬпɪинɚɞлɟжноɫɬипɪоизɜоɞнымɮлɚɜонолɚȺнɚлизɫɬɪɭкɬɭɪɫоɟɞинɟнийоɫɭщɟɫɬɜлялимɟɬоɞомɫпɟкɬɪоɫкопииионизиɪɭюкомплɟкɫооɛɪɚзɭющимиɞоɛɚɜкɚмипоɫкольмɚкɫимɚльноинɮоɪмɚɬиɜнɚɞоɫɬоɜɟɪнɚ10ɍɎ-ɫпɟкɬɪыɚɝликоноɜɜɟщɟɫɬɜɚɯɚɪɚкɬɟɪизɭюɬɫянɚличиɟмɞɜɭɯполоɫоɞинɚкоɜойинɬɟнɫиɜноɫɬиПолɭчɟнныɟɫɜиɞɟɬɟльɫɬɜɭɬомчɬоɫоɟɞинɟнийположɟниипɪиɫɭɬɫɬɜɭɟɬɫɜоɛоɞнɚяɝиɞɪокɫиɝɪɭппɚчɬоɭкɚзыɜɚɟɬзнɚчиɬɟльныйɛɚɬоɯɪомныйоɛɟиɯполоɫпоɝлощɟнияпɪиɫɭɬɫɬɜииɚлюминияȼɟщɟɫɬɜɚпɪиɫɭɬɫɬɜииɚлюминияɞɚюɬзнɚчиɬɟльныйɛɚɬоɯɪомныйоɛɟиɯполоɫчɬоɫɜиɞɟɬɟльɫɬɜɭɟɬнɚличииɝиɞɪокɫиɝɪɭппɞоɛɚɜлɟнииɫɜɟжɟплɚɜлɟнноɝонɚɬɪияɚцɟɬɚɚнɚлизиɪɭɟмымɪɚɫɬɜоɪɚмɜɟщɟɫɬɜнɚɛлюɞɚɟɬɫяɛɚɬоɯɪомныйкоɪоɬкоɜолноɜой II полоɫыэɬоɫɜиɞɟɬɟльɫɬɜɭɟɬнɚличииɫɜоɛоɞнойɝиɞɪокɫиɝɪɭппыɜɫɟɯɫоɟɞинɟнийȻɚɬоɯɪомɞлинноɜолноɜойполоɫыɫпɟкɬɪɚɯщɟɫɬɜɜызыɜɚɟɬɞоɛɚɜлɟниɟɫɜɟжɟплɚɜлɟнноɚцɟɬɚɬɚнɚɬɪиякиɫлоɬычɬоɯɚɪɚкɬɟɪноɮлɚɜоноиɞоɜоɪɬоɞиɝиɞɪокɫиɝɪɭппиɪоɜкɭположɟнияɯ 3? 4? ɛокоɜоɝоɮɟнильноɝоɪɚɞикɚлɚȻɚɬоɯɪомный I-полоɫыɛɟзɫнижɟнияинɬɟнɫиɜноɫɬинɚɛлюɞɚɟɬɫяпоɞɜлияниɟммɟɬилɚɬɚнɚɬɪиячɬоɭкɚзыɜɚɟɬнɚличиɟɝиɞɪокɫиɝɪɭппɚɬомоɜɫнижɟниɟинɬɟнɫиɜноɫɬичɟɪɟз 3-5 ɜɟщɟɫɬɜɬɚкжɟнɚɛлюɞɚɟɬɫяɛɚɬоɯɪомный I-полоɫыɛɟзизмɟнɟнияинɬɟнɫиɜноɫɬичɬоɫɜиɞɟɬɟльɫɬɜɭɟɬɫɜоɛоɞнойɝиɞɪокɫиɝɪɭппɟɫоɟɞинɟнияɯ I-Ⱦля ɭɬочнɟнияположɟнияɭɝлɟɜоɞноɝооɫɬɚɬкɚиɫпользоɜɚлиɫпɟкɬɪоɫкопиюɚɝликонɚпɪиɫɭɬɚлюминияɫпɟкɬɪоɜпоɝлощɟнияɜɟщɟɫɬɜпоɫлɟɝиɞɪолизɚɫɟɛяɜнимɚниɟпояɜлɟниɟɛɚɬоɯɪомноɝоɚɝликонɚчɬооɫноɜɚниɟɭɬɜɟɪжɞɚɬьчɬоɫоɟɞинɟнияɯмɟɫɬоɝликозиɞиɪоɜɚниɟположɟниюɫпɟкɬɪыизмɟɪялиɮɭɪьɟɫпɟкɬɪомɟɬɪ 1201?. Кɚɪɛонильнɚяɝɪɭппɚзɚмɟщɟнноɝоɮлɚɜонɚпоɝлощɚɟɬ 1650 ɮлɚɜонɫоɞɟɪжиɬɝɪɭппɭположɟниичɚɫɬоɬɚпонижɚɟɬɫя 1619 ȼɜɟɞɟниɟɝɪɭппыположɟнииɮлɚɜоноиɞоɜɫколькоɭɜɟличиɜɚɟɬчɚɫɬоɬɭпоɝлощɟниякɚɪɛонильɝɪɭппы 1655 нɟɫмоɬɪяɜозможноɫɬьоɛɪɚзоɜɚнияɜнɭɬɪимолɟкɭляɪнойɜоɞоɪоɞнойɫɜязи 1655 ȼ ɪɟзɭльɬɚɬɟщɟлочноɝоиɫɫлɟɞɭɚɝликоноɜкɚлияɝиɞɪокɫиɞомполɭчɟныпɪоɬокɚɬɟɯоɜɚякиɫлоɬɚɮлоɪоɝлюцинɪɟзɭльɬɚɬɟщɟлочноɝоɚɝликоноɜкɚлияɝиɞɪокɫиɞомполɭчɟныɝиɞɪокɫиɛɟнзойнɚякиɫлоɬɚɮлоɪоɝлюцинчɬоɞокɚзɚнопоɞɜижнɚя ? Ȼɍȼ (4:1:5), пɪояɜиɬɟль ? 1% ɪɚɫɬɜоɪжɟлɟзɚ (III), 0,1% ɞиɚзоɬиɪоɜɚннɚяɫɭльɮɚнилоɜɚякиɫлоɬɚ (III), 0,1% ȼ ɪɟзɭльɬɚɬɟполноɝокиɫлоɬноɝоɝиɞɪолизɚщɟɫɬɜɚполɭчɟныɚɝликон ? кɜɟɪцɟɬинɝɚлɚкɬозɚȽиɞɪолизɚɬоɛɪɚɛɚɬыɜɚлиэɮиɪомɜоɪонкɟМɟɬоɞомпользоɜɚниɟмɫɬɚнɞɚɪɬноɝо ? кɜɟɪцɟɬинɚпоɞɜижныɯȻɍȼ (4:1:5) 15% ɭкɫɭɫнойлоɬыɯɪомɚɬоɝɪɚɮиɪоɜɚлиполɭчɟнноɟизɜлɟчɟниɟȽиɞɪолизɚɬнɟйɬɪɚлизоɜɚликɚɪɛонɚɬомɭниɜɟɪɫɚльномɭинɞикɚɬоɪɭɮильɬɪоɜɚлиɭпɚɪиɜɚнɟɛольшоɝооɫɬɚɬкɚɯɪомɚɬоɝɪɚɮиɪоɜɚлипоɞɜижнойȻɍȼ (4:1:5) иɫпользоɜɚниɟмɫɬɚнɞɚɪɬныɯПоɫлɟпɪояɜлɟнияɚнилинɮɬɚлɚɬɪɟɚкɬиɜомоɛнɚɪɭжилиɝɚлɚкɬозɭ (4:1:5) ȼ ЯМɊ-1Н-ɫпɟкɬɪɟ (400MHz, DMSO-dɜɟщɟɫɬɜɚпɪояɜляюɬɫяɫиɝнɚлыпɪоɬоноɜ125Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134125ɋпɟкɬɪɜɟщɟɫɬɜɚ); 115,58 (Ɍɚкимоɛɪɚзомɫпɟкɬɪоɫкопиипоɞɬɜɟɪжɞɚюɬɜыɜоɞыполɭчɟнныɟоɫноɜɚнииɫпɟкɬɪоɮоɬомɟɬɪииионизиɪɭющикомплɟкɫооɛɪɚзɭющимиɞоɛɚɜкɚмиɫпɟкɬɪɜɟщɟɫɬɜɚɯɚɪɚкɬɟɪизɭɟɬɫяɬɪɟмялоɫɚмипоɝлощɟниямɚкɫимɭмɚми 216, 243, 325 ɫпɟкɬɪоɫкопииɛɭмɚжнойɯɪомɚɬоɝɪɚɮииможноɫɞɟлɚɬьɜыɜоɞчɬоɜɟщɟɫɬɜоэɬокоɮɟйнɚякиɫлоɬɚ 216, 243, 325 Полɭчɟнныɟɪɟзɭльɬɚɬыɫɜиɞɟɬɟльɫɬɜɭюɬчɬоɜɟщɟɫɬɜо ? эɬо 3,5,7,3?,4?-пɟнɬɚокɫиɮлɚɜонкɜɟɪɜɟщɟɫɬɜо ? кɜɟɪцɟɬинɝɚлɚкɬоɫоɟɞинɟниɟкɜɟɪцɟɬинɪɭɬиɪɭɬинɫоɟɞинɟниɟпɪɟɞɫɬɚɜляɟɬɬɟɬɪɚокɫиɮлɚɜонкɟмпɮɟɪолɜɟщɟɫɬɜокɟмпɮɟɪолɝлюкопиɪɚнозиɞɚɫɬɪɚɝɚлинɜɟщɟɫɬɜокоɮɟйнɚякиɫлоɬɚОпɪɟɞɟлɟниɟкоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияКоэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияокɬɚнолɜоɞɚочищɟннɚяшиɪокоиɫпользɭɟɬɫямɟɞицинɟɮɚɪмɚколоɝииопиɫɚнияɬɪɚнɫпоɪɬɚɜɟщɟɫɬɜчɟɪɟзɛиолоɝичɟɫкиɟ ? Покɚзɚночɬозɚɜиɫимоɫɬькоэɮɮициɟнɬомɪɚɫпɪɟɞɟлɟнияɛиолоɝичɟɫкимможɟɬноɫиɬьпɚɪɚɛоличɟɫкийɯɚɪɚкɬɟɪэɬомɞоɫɬижɟнииопɬимɭмɚлипоɮильноɫɬиɞɚльнɟйшɟɟможɟɬпɪиɜоɞиɬьɫнижɟниюɛиолоɝичɟɫкойɚкɬиɜноɫɬи [17]. ɊɟзɭльɬɚɬыопɪɟɞɟлɟниякоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияпɪɟɞɫɬɚɜлɟныɬɚɛлицɚɯɌɚɛлицɚКоэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияɫиɫɬɟмɚɯɜоɞɚочищɟннɚяокɬɚнолɜоɞɚочищɟннɚяɞиэɬилоɜыйэɮиɪиɫɯоɞноɟэкɫɬɪɚкцияэɮиɪом0,10,420,370,070,030,20,750,710,180,020,41,391,050,290,11экɫɬɪɚкцияокɬɚнолом0,61,951,890,590,010,82,582,470,780,021,03,172,870,900,10Пɪимɟчɚниɟ ? иɫɯоɞнɚяконцɟнɬɪɚцияɫопɪоɬиɜлɟниɟɜоɞныɯɪɚɫɬɜоɪоɜ ? ɪɚɜноɜɟɫныɟконцɟнɬɪɚɫɬɜоɪоɜпоɫлɟэкɫɬɪɚкцииɌɚɛлицɚɫɪɟɞыкоэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияɫиɫɬɟмɟɜоɞɚочищɟннɚяокɬɚнолиɫɯоɞноɟиɫɯоɞноɟокɬɚнолиɫɯоɞноɟокɬɚнол0,60,986,866,573,433,100,570,590,81,786,856,412,592,480,750,761,02,186,724,063,182,450,600,77КоэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияɪɚɫɫчиɬыɜɚличɟɪɟзɪɚɜноɜɟɫныɟконцɟнɬɪɚцииɜоɞɟочиокɬɚнолɟКонцɟнɬɪɚцияɬɚнольноɝоɪɚɫɬɜоɪɚɜозɜоɞилɚɫьоɬɜɟɬɫɬɜɭющɟйПоɫлɟɞняяопɪɟɞɟлялɚɫьɝɪɚɮичɟɫкимɫпоɫоɛомɪиɫɭнкɟ 1 покɚзɚнɚлинɟйноɫɬьɬɚкоймоɫɬипозɜоляющɚяɝɪɚɮичɟɫкиопɪɟɞɟлиɬьɜɟличинɭкоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияПолɭчɟнныɟɫɜиɞɟɬɟльɫɬɜɭюɬчɬоɪɚɫɬɜоɪоɜкɪыжоɜникɚɪɚɫпɪɟɞɟлɟниɟɜоɞноɝоɫлояоɪɝɚничɟɫкийпɪоиɫɯоɞиɬокɬɚнолЭкɫпɟɪимɟнɬɚльныɟɜлиянииɪɚɫпɪɟɞɟлɟниɟɫɪɟɞыпокɚзɚличɬокɪыжоɜникɚпɪɟɞɟлɟниɟпɪоиɫɯоɞиɬщɟлочнойɫɪɟɞɟɬɚɛлɌɚɛлицɚЗнɚчɟниякоэɮɮициɟнɬɚɪɚɫпɪɟɞɟлɟнияɪɚзличныɯɫɪɟɞɚɯ№Коэɮɮициɟнɬɪɚɫпɪɟɞɟлɟнияɋɪɟɞɚ11,59ɜоɞɚочищɟннɚяокɬɚнол22,60ɜоɞɚочищɟннɚяэɮиɪ31,84ɜоɞɚочищɟннɚяокɬɚнол41,43ɜоɞɚочищɟннɚяокɬɚнол126ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияɊиɫɭнокȽɪɚɮичɟɫкийɪɚɫчɟɬпɚɪɚмɟɬɪоɜɪɚɫпɪɟɞɟлɟниякɪыжоɜникɚоɬклонɟнноɝоПɪимɟчɚниɟɪɚɜноɜɟɫныɟконцɟнɬɪɚцииɪɚɫɬɜоɪоɜпоɫлɟэкɫɬɪɚкцииПокɚзɚночɬопɪоцɟɫɫпɟɪɟɯоɞɚɫкɭюɮɚзɭокɬɚнолноɫиɬэкзоɬɟɪмичɟɫкийɯɚɪɚкизмɟнɟнииизоɛɚɪноизоɬɟɪмичɟɫкопоɬɟнциɚлɚэнɬɚльпийнойɫоɫɬɚɜляющɟйɛольшɟэнɬɪопийноɝоɮɚкɬоɪɚпоэɬомɭЭɬоɫɜиɞɟɬɟльɫɬɜɭɟɬɫɚмопɪоизɜольноɫɬипɪоцɟɫɫɚɪɚɫпɪɟɞɟлɟнияполиɫɚɯɚɪиɞоɜоɪɝɚничɟɫкийпɟкɬиноɜыɯɜɟщɟɫɬɜɜыɞɟлɟнныɯкɪыжоɜникɚоɬклонɟнноɝоɯолɟɫɬɟɪиноɜыйɝɪɭппыинɬɚкɬныɯɫоɞɟɪжɚниɟоɛщɟɝолɟɫɬɟɪинɚɯолɟɫɬɟɪинɚɯолɟɫɬɟɪинɚɫоɫɬɚɜляло 2,42?0,08 ммольммоль 0,47?0,03 ммольɫооɬɜɟɬɫɬɜɟнноɊиɫɭнокɯолɟɫɬɟɪиноɜыйПɪимɟчɚниɟоɛщоɛщийɯолɟɫɬɟɪин ? ɯолɟɫɬɟɪиннизкойплоɬноɫɬиɯолɟɫɬɟɪинɜыɫокойплоɬноɫɬи ? ɬɪиɝлицɟɪиɞы ? инɬɚкɬныɟжиɜоɬныɟ ? конɬɪольпɟкɬиноɜыɟɜɟщɟɫɬɜɚɜыɞɟлɟнныɟкɪыжоɜникɚоɬклонɟнноɝоɫɬɚɬиɫɬичɟɫкизнɚчимоɋɬьюɞɟнɬɚоɬноɫиɬɟльноɝɪɭппыжиɜоɬныɯɫɬɚɬиɫɬичɟɫкизнɚчимоɋɬьюɞɟнɬɚоɬноɫиɬɟльноɝɪɭппыжиɜоɬныɯ127Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134127 By methods of high-perfor- ed by the presence of standards. avonoids, coumarins and phe-avonoids, coumarins and phe-For more detailed information on the polyphenol ex-tract from European gooseberry leavesGrossularia rec- (L.) Mill.), obtained by extraction with 40% ethyl chromatography. cient of the substances under study uids [2].Pectic substances (PS) have a preventive effect: an- ammatory, antibacterial, anti-sclerotic, anti-aller-gic. They have the property of lipid metabolism correc-They have the property of lipid metabolism correc-THE AIM OF THE WORK is isolation, identi cient and the antidislipidemic activity of the Gros-sularia reclinataMATERIALS AND METHODSEuropean gooseberry leaves collected in July-August owering and fruiting, the ?Mos-cow red? variety (the region of the Caucasian Mineral Wa-ters (Stavropol Territory)) were used as the test material. by heating for 60 minutes. The resulting recovery was treated with chloroform, diethyl ether, ethyl acetate and tions were transferred to chromatographic columns lled with silica gel of the Silicagel 60 brand or poly-amide of the Polyamide Woelm brand. The eluent for ed water, and for silica gel it was chloroform. The elution process was monitored by l? plates) and paper chromatography (PC) (MUNKTELL FN 7). As the mobile phase n-butanol: acetic acid: water (BAW ter (5: 1: 1) were used. cient substances from European goose-kov and M. Sinner?s methods [6]. After hydrolysis, glu-ysis indicated PS predominance (6.4%). Therefore, fur--When determining the distribution coef? cient from organic solvents, diethyl ether and n-octanol were used, экɫпɟɪимɟнɬɚльнойɝипɟɪɯолɟɫɬɟɪинɟмиижиɜоɬныɯɝɪɭппынɟɝɚɬиɜноɝоконɬɪоляинɬɚкɬнымикɪыɫɚмиоɬмɟчɟноконцɟнɬɪɚцииоɛщɟɝоɯолɟɫɬɟɪинɚɯолɟɫɬɟ 95,5% (p)() 110,6% (p)ɫооɬɜɟɬɫɬɜɟнноɋоɞɟɪжɚниɟлɟɫɬɟɪинɚɝɪɭппыжиɜоɬныɯнɚпɪоɬиɜɭмɟньшилоɫьоɬношɟниюкɪыɫɚм 86,5% чɬоɫоɝлɚɫɭɟɬɫялиɬɟɪɚɬɭɪными 86,5% Поɞпɟкɬиноɜыɯɜɟщɟɫɬɜполɭчɟнкɪыжоɜникɚжиɜоɬнымиɝɪɭппынɚɛлюɞɚлɚɫьноɪмɚлизɚцияɯолɟɫɬɟɪиноɜоɝоɜыɪɚжɚɟмɚяɫнижɟнииконцɟнɬɪɚцииоɛщɟɝоɯолɟɯолɟɫɬɟɪинɚ 27,1% (p)ɫооɬɜɟɬɫɬɜɟнноɬɚкɫоɞɟɪжɚнияɯолɟɫɬɟɪинɚПолɭчɟнныɟɯоɞɟиɫɫлɟɞоɜɚнияɝлɚɫɭюɬɫялиɬɟɪɚɬɭɪыпоɞɬɜɟɪжɞɚюɬɛлɚɝопɪияɬноɟɜлияниɟпɟкɬиноɜыɯɜɟщɟɫɬɜɯолɟɫɬɟɪиноɜыйоɛмɟн [18]. ɋнижɟниɟоɛщɟɝоɯолɟɫɬɟɪинɚɬɪиɝлицɟɪиɞоɜноɪмɚлизɚцияоɬношɟнияɯол / ɯолɜɜɟɞɟнияизɭчɚɟмыɯпɟкɬиноɜыɯɜɟщɟɫɬɜɜозможноɫɜязɚнопɪоцɟɫɫɚɜɫɚɫыɜɚнияизɛыɬкɚɯолɟɫɬɟɪиɬɪиɝлицɟɪиɞоɜчɬоɫɜоюочɟɪɟɞьпɪɟɞоɬɜɪɚпɪоɚɬɟɪоɝɟнныɟэɮɮɟкɬы / ЗȺКЛЮЧȿНИȿМɟɬоɞомпɪɟпɚɪɚɬиɜнойколоночɯɪомɚɬоɝɪɚɮииизɜлɟчɟниикɪыжоɜникɚоɬклонɟнноɝоGrossularia reclinata (L.) Mill.полɭчɟнноɝоɫпиɪɬом 40%, ɪɭжɟныкɜɟɪцɟɬинɪɭɬинкɟмпɮɟɪолɚɫɬɪɚɝɚлинкоɮɟйнɚякиɫлоɬɚȽипɟɪозиɞɪɭɬинкоɮɟйнɚякиɫлоɬɚкɪыжоɜникɚоɬклонɟнноɝоОпɪɟɞɟлɟниɟɜɟличинкоэɮɮициɟнɬоɜɪɚɫпɪɟɞɟпɟкɬиноɜыɯɜɟщɟɫɬɜɫиɫɬɟмɚɯзɚɜиɫимоɫɬькиɫлоɬноɫɬиɫɪɟɞыпозɜолиɬɝнозиɪоɜɚɬьɜозможноɫɬьпɪоникноɜɟниячɟɪɟзɛиолоɝичɟɫкиɟɋлɟɞоɜɚɬɟльноможнопɪɟɞположиɬьчɬокɪыжоɜникɚлочнойɫɪɟɞɟкишɟчноɝоɫоɞɟɪжимоɝоɛɭɞɭɬɪɚɫпɪɟɞɟляɬьɫяɜɫɚɫыɜɚɬьɫяэкɫпɟɪимɟнɬɚльнойɝипɟɪɯолɟɫɬɟɪинɟмииоɬмɟчɚɟɬɫяɭɜɟличɟниɟконцɟнɬɪɚцииоɛщɟɝоɯолɟɫɬɟɪинɚɯолɟ 95,5% (p)() 110,6% (p)ɫооɬɜɟɬɫɬɜɟнноɬɚкжɟɫнижɟниɟɫоɞɟɪжɚнияɯолɟɫɬɟɪинɚиɫɫлɟɞɭɟмыɯпɟкɬиноɜыɯɜɟщɟɫɬɜкɪыжоɜникɚоɬклонɟнноɝоɫпоɫоɛɫɬɜоɜɚлокоɪɪɟкцииноɪмɚлизɚконцɟнɬɪɚцииоɛщɟɝоɯолɟɫɬɟɪинɚɯолɟɫɬɟɪинɚɯолɟɫɬɟɪинɚ128ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияhydroxide, respectively. and the electrolyte were taken equally. The concentration nate was determined by the conductometric method us-nate was determined by the conductometric method us-Determination of antidislipemic activityAnimalsDetermination of the lipid-cholesterol pro? le of blood was performed on 30 male Wistar rats weighing 200-220 grams, divided into 3 equal groups (n = 10). The animal care and the experiments were in accordance with the rules of laboratory practice (GLP) for pre-clinical studies in the Russian Federation (GOST 3 51000.396 and 51000.4-96), tion for the Protection of Vertebrates (1997).Investigations of the effect of pectic substances iso- rst group of rats were intact animals (IA). In ower oil [8]. Hereby, the second group of rats were negative con-port. The third group of rats were injected per os with the per 100 g of the animal?s weight. le: total cholesterol (TC), tein cholesterol (LDLP cholesterol), high density lipo-protein cholesterol (HDLP cholesterol), and triglycerides (TG). The standard sets of reagents produced by Olvex Diagnosticum (total cholesterol: series 01042017, HDLP cholesterol: series 080022017, LDLP cholesterol: series 20022017, triglycerides: series 01122016, Sweden) were ational statistics methods using STATISTICA 6.0 applica-tion software package (StatSoft, USA). The obtained data iro-Wilk test. In the case of subordination of data to the pare the averages, otherwise further statistical processing RESULTS AND DISCUSSIONumn chromatography. ed water, ethyl alcohol of different concentrations and 95% ethyl alcohol. Two substances were obtained, one of which other had a dark brown color in UV light in the adsorp-Substance 4 having a dark-brown color in UV light in the The ethyl acetate fraction was concentrated to a small volume and mixed with anhydrous chloroform (1:5). The precipitate was transferred onto the column of polyamide, eluted with water-ethanol mixtures with a gradient increase in ethyl alcohol, and Substances 5 and 6 were obtained. The chloroform-ethyl acetate l-tate and transferred to a silica gel column. Elution with chloroform-ethanol mixtures with a gradient increase in ethyl alcohol yielded two fractions: one contained two ond contained four substances ? 9, 10, 11 and 12. Then umns. The elution was carried out with water-ethanol they are individual (Table 1) [9 ? 12].Table 1 ? Values of mobility coef cients of the obtained substancesValue of the mobility coef cient15% acetic acidBAW (4:1:5)0.36?0.020.42?0.030.44?0.030.59?0.040.64?0.020.32?0.020.26?0.020.78?0.040.83?0.040.52?0.030.77?0.030.80?0.030.43?0.020.73?0.020.64?0.020.07?0.010.89?0.030.90?0.02The IR spectrum of Substance 3, as well as the data of comparative chromatographic analysis, UV spectro-photometry with ionizing and complexing additives, are identical to those for Substance 11. This is also indicated by the absence of depression of the melting temperature in the sample of mixing. The similarity of some physical and 129Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134129 avonol. The structural analysis of these compounds was carried out by UV spec- spec-The UV spectra of aglycons (Substances 5 and 10) imately the same intensity. The obtained data indicate cant cant bathochro-all compounds at C7. The bathochromic shift of the avonoids having uence of without changing its intensity, which indicated a free hy-droxy group in C4? in these compounds [10].To clarify the position of the carbohydrate residue, UV spectroscopy of the aglycon in the presence of alumi-num chloride was used. When comparing the absorption spectra of the substances before and after hydrolysis, the cone attracts attention, which suggests that in Compounds trometer FCM 1201?. The carbonyl group of the unsub- avone was absorbed at 1650 cm-1. If a avone cy is reduced to 1619 cm-1. The introduction of a hy- avonoids slightly slightly As a result of alkaline cleavage of the investigated aglycones (2, 3, 5) with potassium hydroxide, protocat-echic acid and ? oroglucin were obtained, and as a result oroglucin were PC (the mobile phase was ? BAW (4: 1: 5), the developer (4: 1: 5), the developer As a result of complete acid hydrolysis of Substance 2, aglucone - quercetin and D-galactose were obtained. The hydrolyzate was treated with diethyl ether in a separatory funnel. The obtained extract was chroma-quercetin sample in the mobile phases of PC BAW (4: 1: 5) and 15% acetic acid. The hydrolyzate was neutral-ized with barium carbonate using a universal indicator, ltered, evaporated to a small residue and chroma-tographed in the mobile phase of PC BAW (4: 1: 5) using standard samples. After the development of anilinphthal-After the development of anilinphthal-The proton signals appear in NMR-1H spectrum (400MHz, DMSO-d6, δ, ppm, J / Hz) of Substance 10: 6.19 (1H, d, 1.96 Hz, H-6), 6 , 37 (1H, d, J = 1.96 Hz, H-8), 8.04 (2H, m, H-2 ?, 6?), 6.82 (2H, m, H-3 ?, 5 ?), 12.94 (1H, s, OH-5).NMR-13C spectrum of Substance 10 (101 MHz, DM-SO-d6): 154.21 (C-2); 135.98 (P-3); 180.52 (P-4); 104.77 9); 162.31 (P-10); 121.55 (C-1?); 130.15 (C-2 & apos;, 6 & apos;); 115.58 (C-3 & apos;, 5?); 157.73 (C-4?). rm our conclusions obtained on the basis of UV spectropho-The UV spectrum of Substance 7 is characterized by According to UV, IR spectroscopy and paper chromatog-raphy, it can be concluded that substance 7 is a coffee fee The results obtained indicate that Substance 5 is 3,5,7,3?,4?-pentaoxy? avone (quercetin), Substance 2 is avone (kaempferol), Substance 8 is kappepro-7 is coffee acid. cient cient in the n-octanol-puri ed water system is widely used in chemistry, medicine, and cient and the biological effect can be biological activity [17]. The results of determining the cient are presented in Tables 2, 3. cient was calculated ed water (C1) and n-octanol (C2). The concentration of the octanol solution was raised to the degree of the corresponding degree of association. The latter was cient and the degree of association.130ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияTable 2 ? Distribution Coef cient in systems water ? n-octanol, ed water-diethyl ether0.10.420.370.070.030.20.750.710.180.020.41.391.050.290.110.61.951.890.590.010.82.582.470.780.021.03.172.870.900.10Note: C is the initial concentration; R is the resistance of aqueous solutions of PS; C and Care the equilibrium concentra-Table 3 ? Effect of pH-medium on the distribution coef cient in the system ed water - n-octanol0.60.986.866.573.433.100.570.590.81.786.856.412.592.480.750.761.02.186.724.063.182.450.600.77are equilibrium concentrations of PC solutions after extractionof PS from European gooseberry, a better distribution from the aqueous layer to the organic layer occurs in n-octanol. Experimental data on the effect on the pH dis-for gooseberry goose is in the alkaline medium (Table 4).131Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-121-134131Table 4 ? Values of the distribution coef cient in various media cientMedium11.59puri ed water ? n-octanol22.60puri ed water ? diethyl ether31.84puri ed water (pH = 2) ? n-octanol41.43puri ed water (pH = 8) ? n-octanolIt has been shown that the process of PC transition into the organic phase (n-octanol) is exothermic (H ) tion of the enthalpy component is greater than the entropy G This indicates the spontaneous distribution of polysaccharides to the organic layer.The effect of pectic substances isolated from European le of bloodlesterol, LDL cholesterol, HDL cholesterol and TG was Figure 2 ? Effect of PC on lipid-cholesterol blood pro leNote: tot. chol. ? total cholesterol, LDL chol. ? cholesterol of low density lipoproteins, HDL chol. ? cholesterol lipoprotein high density, TG-triglycerides, IN ? intact animals, NC ? negative control, PC ? pecti substances isolated from European goose-berry, leaves; cant (p)elative to the group of animals; * ? statistically signi cant (pt-Student criterion) relative to the group of NC animals tration of total cholesterol, LDL-C and TG by 95.5% (p)()10.6% (p)spectively. In the NC group of animals the content of HDL cholesterol, on the contrary, decreased by 86.5% , decreased by 86.5% Under the action of pectic substances obtained from European gooseberry leaves, the normalization of the le of blood was observed in rats, compared with that in the animals of the NC group (Figure 2), expressed in the concentration decrease of total cholesterol, LDL cholesterol and TG by 27.1% tively, and an increase in HDL cholesterol by 32.7% rm the bene cial effect of pectic substances on lipid-cholesterol metabo-fect of pectic substances on lipid-cholesterol metabo-glycerides, normalization of the ratio LDL cholesterol / HDL cholesterol with the input of the studied pectic sub-prevents their pro-atherogenic effects [20, 21]. Preparative column chromatog-raphy was used to extract quercetin, hyperoside, rutin, kaempferol, astragaline and caffeic acid from Europe-Grossularia reclinata (L.) Mill.,obtained by 40% Ethanol. Hyperoside, rutin, coffee 132ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияȻиɛлиоɝɪɚɮичɟɫкийПɟлиɜɚноɜɚɋɟлинɚȺнɞɪɟɟɜɚОɝɚнɟɫянПолиɮɟнольныйɫоɫɬɚɜкɪыжоɜниоɬклонɟнноɝошɟлкоɜицы // НɚɭчныɟɜɟɞомоɫɬиɋɟɪияМɟɞицинɚɎɚɪмɚцияНɚɭчȺльɛɟɪɬИзɛиɪɚɬɟльнɚяɬокɫичноɫɬьМоɫкɜɚОɜоɞоɜпɪɟɞɫɬɚɜлɟнияпɟкɬиноɜыɯɜɟщɟɫɬɜɚɯ // 3. КɪыжɚноɜɫкийȻоɝɞɚноɜичȻɟɫɟɞноɜɚИɜɚнɭшкоȽолоɜɚчɟɜɚȽиполипиɞɟмичɟпɪоɬиɜоɜоɫпɚлиɬɟльныɟэɮɮɟкɬыполиɫɚɯɚɪиɞоɜɛɭɪыɯɜоɞоɪоɫлɟйпɚциɟнɬоɜɎɭнɞɚмɟнɬɚльныɟиɫɫлɟɞоɜɚнияȻɭɛɟнчикоɜɚЛɟɜчɟнкоНɚɫɟɞкинИзɭчɟниɟполиɫɚɯɚɪиɞоɜɬɪɚɜыɯонɞɪиллыɫиɬникоɜиɞɌɪɚɞиционнɚямɟɞицинɚКочɟɬкоɜɛиолоɝичɟɫкиɚкɬиɜныɯɫоɟɞинɟнийПɟлиɜɚноɜɚȺнɞɪɟɟɜɚМыкоцОɝɚнɟɫянПолиɫɚɯɚɪиɞыкɪыжоɜникɚклонɟнноɝо (Grossularia reclinata (L.) Mill.) // ɊɚзɪɚɛоɬкɚиɫɫлɟɞоɜɚниɟмɚɪкɟɬинɝноɜойɮɚɪмɚцɟɜɬичɟɫкойпɪоɞɭкциинɚɭчПяɬиɝоɪɫкɊɭкоɜоɞɫɬɜоэкɫпɟɪимɟнɬɚльномɭɞоклиничɟɫкомɭизɭчɟниюɮɚɪмɚколоɝичɟɫкиɯɜɟщɟɫɬɜ / ПоɞɪɟɞɏɚɛɪиɟɜɚизɞИзɞɚɬɟльɫɬɜоМɟɞицинɚКочɟɬоɜɚɋɟмɟниɫɬɚяɊɟɜинɚОпɪɟɞɟлɟниɟɛиолоɝичɟɫкиɚкɬиɜныɯɫоɟɮɟнольнойполиɮɟнольнойпɪиɪоɞыоɛъɟкɬɚɯмɟɬоɞɚмиɯɪомɚɬоɝɪɚɮииɍɫпɟɯиИɜɚноɜɚɎɟɞоɪоɜɚИɜɚноɜɚɎɟɞоɪоɜОɫɬɪоɭɯоɜɚМɚлкоɜɎлɚɜоноиɞныɟɫоɟɞинɟниякоɪылиɫɬɜɟнницыɫиɛиɪɫкойлиɫɬɜɟнницыɝмɟлинɚ // ɪɚɫɬиɬɟльноɝоКɭличɟнкоȺнɞɪɟɟɜɚЛɭкɚшɭкМɚзɭɪинɚИɫɫлɟɞоɜɚниɟɯимичɟɫкоɝоɫоɫɬɚɜɚɚкɬиɜноɫɬипɟɪиɞɟɪмыклɭɛнɟйкɚɪɬоɮɟляɎɚɪмɚцияɮɚɪмɚколоɝия3. 4(11). DOI: 10.19163/2307-9266-2015-3-4(11)-4-7Ȼомɛɟлɚ T.B., ПɟɬɪичɟнкоКɪоɬкоɜɚ O.A. ɎɟнольныɟɫоɟɞинɟниянɟкоɬоɪыɯɪоɞɚОчɚнкɚɮлоɪыПɟɪмɫкоɝоɪɚɫɬиɬɟльноɝо. 2011. ȻɚнɞюкоɜɚШинкɚɪɟнкоКɚзɚкоɜМɟɬоɞыиɫɫлɟɞоɜɚнияпɪиɪоɞныɯɮлɚɜоноиɞоɜɝоɪɫкИзɞȻɚльнɟолКоɪɭлькинȺɛилоɜМɭзычкинɚɌолɫɬикоɜПɪиɪоɞныɟɮлɚɜоноиɞыНоɜоɫиɛиɪɫкȽɟоȽолоɜкинɊɭɞɟнɫкɚяɌɪоɮимоɜɚȻиолоɝичɟɫкиɚкɬиɜныɟɜɟщɟɫɬɜɚɬɟльноɝопɪоиɫɯожɞɟнияНɚɭкɚЧɟɪɜоннɚяОɝɚнɟɫянȺнɞɪɟɟɜɚɋɟнчɟнкоȻоɪоɜɫкийɋпоɫоɛполɭчɟнияɫɬɚнɞɚɪɬɚпɚɬɭлɟɬинɚɫоцɜɟɬийɛɚɪɯɚɬцɟɜɪɚɫпɪоɫɬɟɪɬыɯ (Tagetes patula L.) // Зɞоɪоɜьɟоɛɪɚзоɜɚниɟ XXI ɜɟкɟКолɛинɋоломиноɜɚɌюɪинɚКɪылоɜɚɊɚɫчɟɬɜɟɪиɮикɚциякоэɮɮициɟнɬоɜокɬɚнолɜоɞɚɝɟɪɛициɞоɜɯимичɟɫкийжɭɪнɚлKu C.S., Kim B, Pham T.X., Yang Y., Weller C.L., Carr T.P., Park Y.K., Lee J.Y. Hypolipidemic Effect of a Blue-Green Alga (Nostoc commune) Is Attributed to Its Nonlipid Fraction by Decreasing Intestinal Cholesterol Absorption in C57BL/6J Mice // Journal of Medicinal Food. 2015. 18(11). P. 1214 ? 1222. DOI: 10.1089/Jiang T., Gao X., Wu C., Tian F., Lei Q., Bi J., Xie B., Wang H.Y., Chen S., Wang X. Apple-Derived Pectin Modulates Gut Microbiota, Improves Gut Barrier Function, and Attenuates Metabolic Endotoxemia in Rats with Diet-Induced Obesity Mice // Nutrients. 2016. 8(3). P. 126.Jesch E.D., Carr T.P. Food Ingredients That Inhibit Cholesterol Absorption // Preventive Nutrition and Food Science. 2017. 22(2). P. 67-80. DOI: 10.3746/pnf.2017.22.2.67Jakobsdottir G., Xu J., Molin G., Ahrne S., Numan M. High-Fat Diet Reduces the Formation of Butyrate, but ammation, Liver Fat and Cholesterol in Rats, while Dietary Fibre Counteracts These Effects // . 2013. No. 8(11): e80476. rst isolated from European gooseberry cients values biological membranes. 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Raschet i veri kaciya koef cientov raspredeleniya ok-cientov raspredeleniya ok-? cation of herbicide distribution coef cient between n-octanol and Ku CS, Kim B, Pham TX, Yang Y, Weller CL, Carr TP, Park YK, Lee JY. Hypolipidemic Effect of a Blue-Green Alga (Nostoc commune) Is Attributed to Its Nonlipid Fraction by Decreasing Intestinal Cholesterol Absorption in C57BL/6J Mice. Journal of Medicinal Food. 2015 Nov;18(11):1214-22. DOI: 10.1089/jmf.2014.0121Jiang T, Gao X, Wu C, Tian F, Lei Q, Bi J, Xie B, Wang HY, Chen S, Wang X. Apple-Derived Pectin Modulates Gut Microbiota, Improves Gut Barrier Function, and Attenuates Metabolic Endotoxemia in Rats with Diet-In-Jesch ED, Carr TP. Food Ingredients That Inhibit Cholesterol Absorption. Preventive Nutrition and Food Science. Jakobsdottir G, Xu J, Molin G, Ahrne S, Numan M. High-Fat Diet Reduces the Formation of Butyrate, but ammation, Liver Fat and Cholesterol in Rats, while Dietary Fibre Counteracts These Effects. PLoS ONE. 2013 Nov 13;8(11):e80476. DOI: 10.1371/journal.pone.008047134ɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɎɚɪмɚцияɮɚɪмɚколоɝияКонɮликɬинɬɟɪɟɫоɜȺɜɬоɪызɚяɜляюɬоɬɫɭɬɫɬɜииконɮликɬɚинɬɟɪɟɫоɜȺɞжиɚɯмɟɬоɜɚЛɟонɬьɟɜнɚ ? ɞɚɜɚɬɟлькɚɮɟɞɪыоɪɝɚничɟɫкойПяɬиɝоɪɫкомɟɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ɚлɚȼолɝȽМɍМинзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯинɬɟɪɟɫоɜпɪиɪоɞныɯɫоɟɞинɟнийПозɞнякоɜ ? пɪɟпоɞɚɜɚɬɟлькɚɮɟɞɪыɮɚɪмɚколоɝиикɭɪɫомклиничɟɫкойɮɚɪмɚколоɝииПяɬиɝоɪɫкоɝомɟɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ɮилиɚлɚȼолɝȽМɍМинзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯинɬɟɪɟɫоɜɜɟщɟɫɬɜлɚɞɚющиɯэнɞоɬɟлиопɪоɬɟкɬиɜнойɚкɬиɜноɫɬьюE-mail: pozdniackow.dmitry@yandex.ruЧɟɪɜоннɚяНɚɞɟжɞɚМиɯɚйлоɜнɚ ? кɚɮɟɞɪыоɪɝɚничɟɫкойПяɬиɝоɪɫкоɝоɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ɮилиɚлɚȼолɝȽМɍМинзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯɬɟɪɟɫоɜпɪиɪоɞныɯɫоɟɞинɟнийМыкоцПɟɬɪоɜнɚ ? кɚнɞиɞɚɬɮɚɪмɚцɟɜɬичɟɫкиɯнɚɭкɞоцɟнɬкɚɮɟɞɪынɟоɪɝɚничɟɫкойɮизичɟɫкойколлоиɞнойПяɬиɝоɪɫкоɝоɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ɮилиɚлɚȼолɝȽМɍМинзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯɪɟɫоɜɮизикоɯимичɟɫкиɟɫɜойɫɬɜɚпɪиɪоɞныɯɫоɟȼоɪонкоɜȼлɚɞиɫлɚɜоɜич ? ɞокɬоɪнɚɭкзɚɜɟɞɭющийкɚɮɟɞɪойɮɚɪмɚколоɝиикɭɪɫомклиничɟɫкойɮɚɪмɚколоɝииПяɬиɝоɪɫкоɝомɟɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ȼолɝȽМɍМинзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯинɬɟɪɟɫоɜɜɟщɟɫɬɜоɛлɚɞɚющиɯэнɞоɬɟлиопɪоɬɟкɬиɜнойɚкɬиɜноɫɬьюɪɚзɪɚɛоɬкɚɮɚɪмɚколоɝичɟɫкойкоɪɪɟкцииɫоɫɬоянийɜозникɚюиɫпыɬыɜɚющиɯпоɫɬоянноɟэкɫɬɪɟмɚльɮизичɟɫкоɟпɫиɯоэмоционɚльноɟнɚпɪяжɟниɟɬомчиɫлɟɫпоɪɬɟɜыɫокиɯɞоɫɬижɟнийa.v.voronkov@pmedpharm.ruОɝɚнɟɫянЭɞɭɚɪɞɌоникоɜич ? ɞокɬоɪɮɚɪмɚцɟɜɬичɟɫкиɯнɚɭкпɪоɮɟɫɫоɪзɚɜɟɞɭющийкɚɮɟɞɪойоɪɝɚничɟɫкойПяɬиɝоɪɫкоɝомɟɞикоɮɚɪмɚцɟɜɬичɟɫкоɝоинɫɬиɬɭɬɚ ? ɮилиɚлɚȼолɝȽМɍзɞɪɚɜɚɊоɫɫииОɛлɚɫɬьнɚɭчныɯинɬɟɪɟɫоɜпɪиɪоɞныɯɫоɟɞинɟнийɫинɬɟɬичɟɫкиɯɚнɚлоɝоɜиɫɫлɟɞоɜɚниɟпɪомышлɟнныɯоɬɯоɞоɜпищɟɜоɝоɮɚɪмɚцɟɜɬичɟɫкоɝопɪоизɜоɞɫɬɜɚкɚкɞополниɬɟльиɫɬочникɚполɭчɟниялɟчɟɛнопɪоɮилɚкɬичɟɫɪɟɞɫɬɜмɟɞицинɫкɚя. E-mail: edwardov@ПоɫɬɭпилɚɪɟɞɚкциюОɬпɪɚɜлɟнɚɞоɪɚɛоɬкɭПɪиняɬɚпɟчɚɬи ict of interest ict of interest.? Teacher of the Department of Organic Chemistry, Pyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State University. Research interests: chemistry Teacher of the De-Pharmacology, Pyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State Univer-sity. Research interests: search for substances of endo-thelioprotective activity. E-mail: pozdniackow.dmitry@ - postgradu-ate student of the Department of Organic Chemistry, Py-of Volgograd Medical State University. Research inter-? PhD (Pharmacy), Asso-ciate Professor of the Department of Inorganic, Physical and Colloid Chemistry, Pyatigorsk Medical and Pharma-ceutical Institute, a branch of Volgograd Medical State University. Research interests: physical and chemical properties of natural compounds. E-mail: 58041@mail.Voronkov Andrey Vladislavovich course of clinical pharmacology,Pharmaceutical Institute, a branch of Volgograd Medi-cal State University. Research interests: search for sub-stances with endothelioprotective activity; development of ways of pharmacological correction of conditions arising in individuals experiencing constant extreme physical and psychoemotional stress, including sports innovative approaches in the sphere of postgraduate ed-ucation specialists. E-mail: prohor.77@mail.ruOganesyan Eduard Tonikovich Professor, Head of the Department of Organic Chem-Volgograd Medical State University.Research interests: alogues; research of industrial wastes of food and phar-maceutical production as an additional source of medi-cal and prophylactic drugs, medical chemistry. E-mail: edwardov@mail.ru
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About the authors

S. L. Adjiahmetova

Pyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State University

Email: fake@neicon.ru

D. I. Pozdnyakov

Pyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State University

Email: pozdniackow.dmitry@yandex.ru

N. M. Chervonnaya

Pyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State University

Email: nadezhda.chervonnaya@yandex.ru

L. P. Myikots

Pyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State University

Email: 58041@mail.ru

A. V. Voronkov

Pyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State University

Email: a.v.voronkov@pmedpharm.ru

E. T. Oganesyan

Pyatigorsk Medical and Pharmaceutical Institute, a branch of Volgograd Medical State University

Email: edwardov@mail.ru

References

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