Vol 20, No 1-2 (2020)

Clinical Medicine
Morphological features of the placenta in pregnant women with excessive gestational weight gain
Vachrushina A.S., Krivenko A.S., Moiseenkova S.D., Ogareva A.S., Pokusaeva V.N.
Abstract

Study objective. To evaluate the association between the placenta and excessive gestational weight gain (GWG).

Materials and methods. A prospective cohort study included the standard ultrasonography with subsequent microscopic morphology of the placenta in term pregnancy. Of 83 examined pregnant women, 46 had excessive GWG and 37 had recommended one. In addition, intensity of lipid infiltration was investigated in 24 placentas (12 in each group).

Study results. Excessive GWG resulted in significant enlargement of placenta which resulted in greater neonatal weight. Ultrasonography and subsequent microscopic evaluation revealed placentas to be less efficient in case of excessive GWG.

Conclusions. These findings indicated that excessive GWG influenced placental morphology. Future studies are necessary to determine accumulation of fat in placentas and membranes in case of excessive GWG, which can be defined as ‘fatty degeneration’.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):6-12
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Myoma of the uterus: the organ-preservation treatment (a review)
Nurmukhametova E.T.
Abstract

Uterine fibroids are one of the main causes of hysterectomy. This radical method of treatment allows to control such symptoms as pain and bleeding, but significantly worsens women’s quality of life, preventing them from their childbearing function. This article includes the results of domestic and foreign studies on organ-preserving methods of treatment of uterine fibroids published within 10 years. The method of uterine artery embolization, which is preferred by an increasing number of doctors and patients suffering from fibroids, is also described in the article. The search for articles was carried out in the databases Pubmed, Web of Science, Scopus.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):13-18
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Auxiliary reproductive technologies in the samara region: efficiency, complications, perspective strands of work
Ratmanov M.A., Benyan A.S., Tshelkovich L.S., Tumina O.V., Balter R.B., Moiseeva I.V., Rudenko J.A., Ilchenko O.A.
Abstract

The paper studies the performance of Samara fertility specialists. It is noted that in 2019 the Samara Region conducted twice as many successful IVF cycles as compared to 2012, when it was 1840 cycles. The clinical pregnancy rate in the region is 47%, which is significantly higher than the same indicator of 38.5% in Russia. The trends of scientific work of reproductologists in Samara are presented in the article. The purposes of their work are the correct choice of the in vitro fertilization protocol, the prevention of complications of induced pregnancy, the restoration of reproductive function after unsuccessful IVF attempts.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):19-27
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Analysis of providing medical care to patients with diabetes in foreign countries
Pervyshin N.A., Lebedeva I.V., Lebedeva E.A.
Abstract

The authors of the article analyzed the publications devoted to the prospects of medical care to diabetic patients abroad. The main attention is paid to gross observational pharmacoeconomical studies as the most effective tool for planning and organization of specialized medical care for patients with diabetes. There is a tendency to increase the role of outpatient care for sufferers, as well as to introduce the elements of standardization, modern information and communication technologies into clinical practice. The challenges of implementation of medical information systems (MIS) to manage the quality of medical care in clinical practice are also pointed out. Proper evaluation of quality parameters is impossible without a complex process of collecting primary medical data with the use of modern computer technology. To ensure the reliability and relevance of information, it is desirable that it is collected and entered into the database by the attending physician directly at the outpatient reception hours. AWPE DM (automated workplace of an endocrinologist for outpatient admission of patients with diabetes mellitus) is a computer program designed to collect medical data of outpatients without additional working time expenditures, followed by a printout of the formalized protocol. Its application in everyday practice allows to solve the local problem of formalization and informational support of primary care to diabetic patients, to systematize the processes of collecting primary medical information. In the future, data provided by AWPE DM can be exported to the Federal Register of Diabetes Mellitus (FRDM) in order to update its database.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):28-36
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Allografts for surgery in the closure of nasal septal intraoperative defects
Shelikhovskaia M.A., Syroezhkin F.A., Tipikin V.P., Vinichenko K.V., Kulish A.V., Kovtun S.V.
Abstract

Surgical correction of the deviation of nasal septum is the most frequently performed surgical interventions in Otorhinolaryngology departments. Sometimes such operations can be complicated by an intraoperative defect in the nasal septum, which is not always possible to close with autogenous tissue. That’s why it has become important to use new allogenic transplant materials in the treatment of these defects. The purpose of the study was to evaluate the effectiveness of allografts (allogeneic fascia and allogenic cartilage treated by Alloplant technology) in closure of intraoperative defects of the nasal septum. The treatment was performed to 40 patients aged 24–55 who developed defect of the mucous membrane of the nasal septum during the operation for nasal spetum deviation. Patients were randomly divided into two groups of 20 people. The 1st group included patients whose allogenic transplantat materials were used in addition to their own tissues during the closure of the defect. The 2nd group consisted of patients whose autogenous tissues were used during the operation. The data of functional state of the nasal mucosa and the anatomical integrity of the nasal septum of all patients before treatment, one month and one year after the treatment were checked and compared. It was revealed that allografts (allogenic fascia and allogenic cartilage treated by Alloplant technology) demonstrated an anti-inflammatory effect, and their use in closing of intraoperative defects of the nasal septum is more effective. This procedure also accelerates improvement of the functional activity of the nasal mucosa in comparison with the use of autogenous tissues solely.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):37-43
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Indicator of stroke volume in estimating patient’s volemic status in the carotid endarterectomy
Prozhoga M.G., Kositsyna G.V.
Abstract

The purpose of the study is to determine the significance of variability index of the heart stroke volume in assessing the volemic status of patients during carotid endarterectomy.

Materials and methods. The study included 60 patients who underwent carotid endarterectomy. The average age of patients is 68 ± 7.4 years. The operation was performed under general anesthesia with mechanical ventilation. Cardiorespiratory interaction was studied. The parameters of the state of central hemodynamics were recorded before and after the functional hemodynamic test with the patient’s legs raised. The patients were divided into 3 groups according to the change in the stroke volume of the heart on the test results.

Results. The 1st group included 11 patients (18%), they have a volemia deficiency in combination with a decrease in myocardial contractility, 18 patients (30%) of the 2nd group suffered deficiency of volemia, and 31 patients (52%) having satisfactory hemodynamics were included into the 3rd group. The results of the test with raising of the legs demonstrated that the changes in the SVV indicator corresponded completely to the changes in stroke volume. It was revealed that, irrespectively of the initial state of hemodynamics, the index of variability of the heart stroke volume correctly reflected the state of the volemic status of patients during carotid endarterectomy.

Findings. The rate of variability of heart stroke volume allows to evaluate the effectiveness of the infusion therapy and to control the volemic load during the carotid endarterectomy operation.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):44-48
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Non-coronarogenic causes of increased cardiac troponins in the practice of physicians (literature review)
Chaulin A.M., Grigorieva Y.V., Milutin I.N., Duplyakov D.V.
Abstract

Cardiac isoforms of troponins are the most sensitive and specific biomarkers of myocardial damage and new high-sensitivity methods can reveal very minor damages on the heart muscle. However, elevated troponin levels indicate cardiac damage, but do not define the cause of the damage. Therefore, cardiac troponin elevations are common in many disease states and do not necessarily indicate the presence of acute myocardial infarction. In clinical practice, interpretation of positive troponins may be challenging. In our article, we consider the main non-coronarogenic causes of increased cardiac troponins. The first part of the review discusses the mechanisms of increase and the diagnostic value of cardiac troponins during physical exertion, inflammatory and toxic myocardial injuries (endocarditis, myocarditis, sepsis), and renal failure. The second part of the literature review describes the mechanisms of increase and the diagnostic value of troponins in pulmonary thromboembolism, dissecting aortic aneurysms, neurogenic pathologies (stroke, subarachnoid hemorrhages), and treatment with cardiotoxic drugs (chemotherapy). Special attention is paid to the false positive causes of elevation of troponins.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):49-61
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Methods for early diagnosis of Parkinson’s disease
Kidalov M.B., Savchenko E.V.
Abstract

Currently, the diagnosis of Parkinson’s disease is carried out mainly on the ground of clinical picture. However, long before the development of motor disorders, a number of signs characteristic of parkinsonism can be detected. The review of the literature presents methods to detect non-motor disorders that can be considered as predictors of the disease and it also discusses the issue of the laboratory studies of specific biomarkers for early diagnosis. We suggested to use the combination of olfactory test and questionnaire about vegetative disorders as the most relevant solution for clinical practice. It is recommended to interpret the test data as a sign of extremely high probability of the development of clinical manifestations of Parkinson’s disease, and, therefore, as a reason to start preventive therapy.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):62-67
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Negative outcome predictors of West syndrome
Prygunova T.M., Antipenko E.A., Mukhin K.Y.
Abstract

West syndrome is infantile epileptic encephalopathy characterized by the triad of symptoms: infantile spasms, changes in the electroencephalogram (EEG) in the form of hypsarrhythmia and psychomotor retardation. Revealing of the predictors of clinical outcome can contribute to the selection of the optimal therapy and determine the terms of dynamic monitoring and improve treatment outcome.

Aim: to identify predictors of West syndrome outcome.

Materials and methods. The study included 132 patients who underwent treatment from 2000 to 2018. At the onset of the observation the age of children was from 5 months to 17 years 11 months. The age of the onset of spasms ranged from 1 day to 3 years 2 months. Depending on the etiological factor patients were divided into 3 groups: group 1 — patients with a structural form (60 patients; 45.5%), group 2 — patients with a genetic form (39 children; 29.5%), group 3 — patients with the disease of unknown etiology (33 children; 25.0%). Evaluating the treatment, special attention was paid to the effectiveness of the first three lines of AEDs and hormones, as well as the further selection of AEDs.

Results. Epileptic spasms were controlled in 76 children (57.6%), all seizures were controlled in 48 (36.4%) children. Patients of the 3rd group demonstrated a higher frequency of spasms arresting (87.9% in comparison to 48.7 and 46.7%) and complete remission (72.7% in comparison to 26.7 and 27.6% respectively). Normal neuropsychic development before the onset of spasms, the absence of epi-activity or the presence of regional epi-activity on the EEG in dynamics, the presence of diffuse changes in MRI had positive prognostic value for spasms control. Neonatal seizures, the presence of epi-activity on the EEG and focal deficiency before spasms, the presence of other seizures, pathology of vision and hearing, and necessity to use ≥2 drugs had negative prognostic value. The best compensation of motor and psychoverbal development was noted in patients who had remission.

Conclusions. Predictors of negative outcome of West syndrome include structural and genetic forms of the disease, neonatal seizures, epi-activity on EEG, impaired neuropsychic development and the focal pathology before spasms, the presence of other seizures, the persistence of epi-activity in dynamics and the absence of the first-line therapy effect.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):68-74
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Congenital cleft lip and palate in children from Tajikistan in 2009–2019
Abdurahmonov A.Z.
Abstract

We analyzed the dynamics of congenital cleft lip and palate in children and studied the archival data of the National Medical Center “Shifobakhsh” (Dushanbe) of the Republic of Tajikistan on the incidence of outpatient visits due to this abnormality in the Republic of Tajikistan over the period from 2009 to 2019. The number of children born with congenital cleft has been increasing for the last ten years. We found out this pathology to develop in families with in-and-in marriage, it also depends upon the sex of a child. Boys are more prone to maxillofacial anomalies.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):75-79
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The results of clinical examination of patients with decompensated form of dental abrasion and complaints of the state of the temporomandibular joints
Gabdrafikov R.R.
Abstract

We examined 66 patients 17–55 years old with decompensated form of dental abrasion, who had applied to the hospital “Dental Forte” with various types of complaints of discomfort in the TMJ. They were divided into several groups: 1st — 17–25 years old, 2nd — 25–35 years old, 3rd — 35–45 years old, 4th — 45–55 years old patients. It was established, that the age of 25–35 is specific for significant indication of dysfunctions in the TMJ. By the age of 35–45 years the number of complaints of discomfort in the TMJ increases. There is also increase in the diversity of complaints, and decrease in their allocation in percentage terms. The number of complaints and their diversity in patients of 45–55 years old remained, complaints of one type of pain decreased, but the number of complaints about “pain” associated with chewing, headache, crunch, etc. increased.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):80-84
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Study of the quality of the marginal gap of artificial crown frames made with the use of traditional and digital technologies
Zhulev E.N., Vokulova Y.A.
Abstract

The aim of the study is to evaluate the quality of the marginal gap of the frames of artificial crowns made with the use of CAD/CAM system and by casting method.

Materials and methods. The article presents an assessment of the quality of the edge fitting of artificial crown frames made by means of CAD/CAM system KaVo ARCTICA on digital images of dentition created by intraoral laser scanner iTero Cadent and frames made of Co-Cr by the method of casting. The Image J computer program was used to study the quality of the marginal gap of artificial crown frames. For statistical analysis of the obtained data nonparametric Wilcoxon-Mann-Whitney test was used for the evaluation of the related samples.

Results. We found that the average value of the marginal gap between the tooth stump of the experimental model and the frames of artificial crowns made in the KaVo ARCTICA Engine milling and grinding machine from the titanium blank Titan-Blank is 21.79 microns. The average value of the cement gap of the frames of artificial crowns made by the traditional casting method is 77.18 microns. The value of the marginal gap of artificial crown frames made by using the CAD/CAM system KaVo ARCTICA was 2.8 times less than the value of the marginal gap of frames made by the traditional casting method with a significance level of р < 0.05 (V-Wilcoxon criterion for related samples = 0, р = 0.001).

Conclusion. The results of our study indicate that the frames of artificial crowns made with the use of CAD/CAM system KaVo ARCTICA have a greater dimensional accuracy of the marginal gap compared to the frames of artificial crowns made by the traditional casting method.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):85-90
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X-ray tеsting of structures of keratinized gum and external cortical plate in the field of intact frontal teeth on the upper jaw
Kostionova-Ovod I.A.
Abstract

Objectives. Сomparison of radiological parameters of the keratinized gum and the external cortical plate in the area of the front teeth on the upper jaw.

Material and methods. Сlinical examination of 50 patients aged 18 to 60 years was carried out in order to perform the work. These patients underwent 3D computed tomography which allowed to study the thickness of the keratinized gums in the dental area 1.1, 1.2, 1.3, 2.1, 2.2, 2.3.

Results. The results of the research revealed a similar trend at each level of measurement for the central and lateral incisors. The average values of the gum thickness decreased in the direction of the tooth crown. The thickness of the outer cortical plate had the lowest values in all studied teeth at the level of the alveolar crest apex and gradually increased in the apical direction. The thinnest external cortical plate and gums were revealed in canines.

Conclusion. The performed study showed that thickness of the gum is directly related to the thickness of the cortical plate. In percentage terms the gum thickness is 132 ± 9.5% of the thickness of the cortical plate.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):91-95
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Analysis of orthopedic treatment of patients with stump pins inserts with artificial crown in significant destruction of the clinical dental crown: the data of archival materials of the dental clinic
Nesterov A.M., Sadykov M.I., Matrosov V.V.
Abstract

The aim of the article is to analyze the orthopedic treatment of patients with a lack of clinical tooth crowns by means of stump pin inserts with an artificial crown presented in the archival materials from the dental clinic of Samara.

Research methods. Health records of dental patients and the orthopaedic work orders of the Dental Clinic No. 2 of the city of Samara over the period of 2015–2019 were analyzed.

Results. Of the total number of patients (142,394 people), 5370 patients underwent a stump pin insert to restore a missing tooth crown. A total of 5802 pin stump tabs were made for these patients. 524 patients (10.09%) had complications. Most complications were observed in the lateral group of patients with prosthetic teeth (62.18%). Discementation of crowns from stump pin inserts was the main complication (53.32%). Ceramic crown chipping from metal-ceramic crowns was observed in 6.46% of all complications.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):96-101
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Application of local drug delivery systems in the treatment of chronic periodontitis and periodontitis, characterized by aggressive course
Osipova Y.L., Akimova S.A., Bulkina N.V., Shastin E.N., Stepanov E.A.
Abstract

The article is devoted to a review of the literature, the purpose of which is to compile and interpret existing data, to identify key issues that need to be resolved before the technology of local delivery system of antimicrobial drugs with controlled release will be widely introduced into clinical practice. The search of the material was carried out by using DBLP, ISI Proceedings, JSTOR Search, Medline, Scopus, Web of Science, Elibrary systems.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):102-107
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Modern view on integrated treatment of patients with chronic localized periodontitis of medium severity (a literature review)
Sinev I.I., Nesterov A.M., Sadykov M.I., Khaikin M.B.
Abstract

This article presents the analysis and systematization of the data presented in literature devoted to the study of condition of periodontal tissues in case of localized periodontitis in people of different age groups, treatment methods of localized periodontitis of moderate severity. The article also provides ideas on the subject and highlights the trends of possible research.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):108-121
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Selection of the urine derivation method in patients with acute urinary retention before surgical treatment of benign prostatic hyperplasia
Gusev D.O., Adilov A.D., Pikalov S.M., Zimichev A.A.
Abstract

Actuality. Nowadays it is necessary to recognize that the problem of acute urinary retention associated with benign prostatic hyperplasia is one of the most frequent causes of hospitalization into the urology hospital. A number of standard urine derivation techniques are used as a part of preoperative treatment.

The aim of the study is to optimize the choice of the preoperative bladder drainage.

Material and methods. The data of 280 patients hospitalized in the Urology Department of Samara City Clinical Hospital No. 8 over the period of 2012–2015 were studied to evaluate the results of acute urinary retention.

Results and conclusions. Retrospective pseudo-factor analysis allows to evaluate the effect of the bladder drainage method on the results of treatment of acute urinary retention in benign prostatic hyperplasia. The results can be used as the basis for a mathematical model which allows to predict the outcome of the treatment of acute urinary retention during surgical treatment of benign prostatic hyperplasia. Obtained data were used to develop recommendations on the methods of bladder drainage as a part of preoperative treatment in patients with benign prostatic hyperplasia.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):122-125
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Pharmacy
Development of suppositories based on aloe arborescens juice
Glushchenko S.N., Shmygareva A.A., Sankov A.N.
Abstract

Introduction. The aim of this work is to develop an effective herbal medicine based on Aloe arborescens juice (Aloe arborescens Mill.) with a high content of active substances. It allows to use this drug for patients suffering from hemorrhoids, as well as to develop methods for the standardization of this dosage form.

Material and methods. Homeopathic suppositories of Aloe DN (containing aloe, rhubarb and chamomile) are recommended by the state registry of medicines for the treatment of chronic constipation. This medicine having some drawbacks, we are the first to develop suppositories with aloe juice containing high level of active substances, in which selected hydrophilic base contributes to the rapid absorption of active substances.

Results and discussion. The advantages of the developed method for producing suppositories with Aloe arborescens juice are as follows: the content of active substances in one suppository on a hydrophilic base (gelatin-glycerol) determined by the UV spectrophotometry method is 2.6 mg, it ensures the optimal pharmacological effect; hydrophilic base, from which the active ingredients of Aloe arborescens juice (barbaloin) are easily absorbed, allows to recommend the use of this dosage form to people with hemorrhoids.

Conclusions. The dosage form of hydrophilic suppositories with Aloe arborescens juice provides a high release of the active substance from the base. This substance is easily absorbed into the rectum and exerts laxative effect. The proposed dosage form is of therapeutic benefit due to the higher concentration of active substances in Aloe arborescens juice (2.6 mg).

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):126-130
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Development of approaches to standardization of black walnut bark
Zimenkina N.I., Kurkin V.A.
Abstract

Significance. Black Walnut (Juglans nigra L.) is a species of trees of the Walnut family (Juglandaceae). This plant of the genus Walnut (Juglans L.) has not been sufficiently studied unlike other species e.g. Juglans regia L. This medicinal plant raw material is quite perspective, its preparations have antimicrobial, general tonic effect. We can use its leaves, unripe fruit, and the bark. However they are not widely used in medicine. In order to introduce the plants of the Walnut genus to the State Pharmacopoeia of the Russian Federation (RF State Pharmacopoeia), it is necessary to conduct of pharmacognostic studies, to develop product specification file to confirm the identification and quality of medicinal plant raw materials.

The aim of this study is to develop a method of quantitative determination of flavonoids in the bark of the black walnut (Juglans nigra L.).

Materials and methods. Material of the study was black walnut bark, stocked during the sap flow period (April) in 2018. The bark was skived up to 15 cm long and 2–3 cm wide. The bark was air-dried with the protection from direct sun light. The end of the drying was checked by the brittleness of the bark.

Results. The methods of the quantitative determination of flavonoids in walnut bark has been developed. We used the differential spectrophotometry taking into consideration state standard sample of rutin at the analytical wavelength of 416 nm. The error of a single determination with a confidence level of 95% is ±1.20%.

Conclusion. We used the developed technique and analyzed a number of samples of black walnut bark. The content of total flavonoids in the plant raw material is 5.13 ± 0.02% (as calculated on rutin). The flavonoid content should be at least 4.0%.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):131-136
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Development of quality standards and standardization of dental medicated film containing succinic acid and cethylpyridinium chloride
Nozhkina N.N., Simonyan E.V., Sinitsky A.I.
Abstract

The quality standards were determined and standardization of model samples of the dental medicated film containing succinic acid and cetylpyridinium chloride was carried out taking into account the requirements of the State Pharmacopoeia of the Russian Federation of the XIV edition.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):137-141
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Study of the elemental composition of the common oat (Avena sativa L.) fruit
Nurullaeva D.K., Farmanova N.T.
Abstract

The article presents the results of a study of the elemental composition of the fruits of common oat (Avena sativa L.) harvested in the Republic of Uzbekistan. The elemental composition was determined by inductively coupled plasma mass spectrometry (ICP-MS). The results of the analysis have shown that potassium, calcium, magnesium, sodium are the dominant elements in the raw material. The elements such as aluminum, iron, manganese, strontium, zinc, honey and lead also present in the studied raw material. The presence of essential elements to a certain degree increases the biological activity of the oat fruit. It is caused by their complex action in combination with main active substances.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):142-145
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Biological activity of new 6-[(1-naphthylmethyl)thio)]-4-chloropyrazolo[3,4-d]pyrimidines
Ofitserova E.S., Alekseeva L.N., Shklyarenko A.A., Yakovlev I.P.
Abstract

Topicality. This article presents the results of the study of the biological activity of newly synthesized 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines. The acute toxicity of 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines was determined experimentally on non-linear white male mice with a total weight of 16–20 g. A generation model was used to experimentally evaluate the analgesic activity acetic “writhing” in male mice. It was experimentally confirmed that the new synthesized 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines were non-toxic and had pronounced analgesic activity.

Aim: to study the biological activity of the new synthesized 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines.

Materials and methods. The prediction of the likely types of biological activity was carried out with the use of PASS computer program. The acute toxicity of 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines was determined experimentally on non-linear white male mice. Analgesic activity was established on a model of acetic acid “writhing” in mice.

Results. 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines were classified as non-toxic substances (LD50 — 2000 mg / kg). The analgesic activity of the test compounds was higher than that of the reference drug.

Conclusion. The new synthesized 6-[(1-naphthylmethyl]thio)-4-chloropyrazolo[3,4-d]pyrimidines are non-toxic and have pronounced analgesic activity.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):146-151
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Determination of antimicrobial activity of alcohol extractions of bark and buds of Quercus robur L.
Ryabov N.A., Kurkin V.A., Ryzhov V.M., Lyamin A.V., Zhestkov A.V., Sokhina A.A.
Abstract

Antimicrobial activity of alcoholic extractions from the aboveground organs like bark and buds of Quercus robur L. was studied. The determination of the minimum inhibitory concentration (MIC) was performed with the use of double serial dilution in broth. The following microorganisms were used as test cultures: Bacillus cereus, Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. It has been revealed that alcoholic extractions of the bark of Quercus robur have the widest spectrum of antibacterial activity in relation to Pseudomonas aeruginosa, Escherichia coli and Candida albicans. Results of microbiological analysis show that alcoholic extractions from the bark and buds have an antimicrobial effect on all of these strains with a single dilution (except for antimicrobial activity against Staphylococcus aureus with 70% extractions of bark of Quercus robur). In particular, relatively high antimicrobial activity was observed in alcoholic extracts of all buds concentrations against Candida albicans at a dilution of 2, 4 and 8 times and Escherichia coli at a dilution of 4, 8 and 16 times. The greatest antimicrobial effect against Pseudomonas aeruginosa has extracts from the bark and buds in all the studied concentrations of alcohol. A significant antimicrobial effect against Escherichia coli has 40% of extractions from the buds of Quercus robur when diluted by 2, 4, 8, 16, 32 and 64 times. The results can be used as justification for the introduction of a new type of raw material that is buds of Quercus robur, as well as drugs based on it in the State Pharmacopoeia of the Russian Federation as antibacterial phytopharmaceuticals.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):152-157
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The development of approaches to the standardization of the syringa vulgaris leaves
Serebryakova A.D., Kurkin V.A.
Abstract

The article studies the problems of phytochemical research of Syringa vulgaris L. leaves, which are a perspective raw material containing flavonoids. As a result of the study, the optimal conditions for the extraction of flavonoids in the leaves of Syringa vulgaris were determined: 70% ethanol was used as an extragent; the ratio “raw material – extragent” was 1 : 50; extraction time was 45 min; the degree of fineness was 1 mm. The method of quantitative determination of the total flavonoids in the leaves of Syringa vulgaris by means of differential spectrophotometry with the use of state standard sample of rutin at a wave length of 412 nm was developed. The content of the total flavonoids for Syringa vulgaris leaves varies from 2,76% to 3,89% (calculated with reference to rutin). The error of a single determination with a 95% confidence probability is 6,12%.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):158-163
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Fruits of Crataegus submollis Sarg.: morphological and anatomical study
Shaikhutdinov I.K., Kurkin V.A., Ryzhov V.M., Tarasenko L.V., Stenyaeva V.V., Zhavkina T.M., Rozno S.A.
Abstract

Actuality. Fruit of Crataegus L. are successfully used in medicine in the Russian Federation as a source of cardiotonic substances. One of the sources of raw material is Crataegus submollis Sarg., which is cultivated as an edible and ornamental plant everywhere. Previous studies revealed that preparations from fruit of Crataegus submollis Sarg. show diuretic activity and antidepressant action. Therefore, we consider Crataegus submollis Sarg. to be of interest as a new source of medicinal plant raw material, its fruit can be used both fresh and dried.

Purpose. The aim of the article is to study the morphological and anatomical features of fresh fruit of Crataegus submollis Sarg.

Materials and methods. The material of the study was the fresh and frozen fruit of Crataegus submollis Sarg. collected in September – October 2018 in the Botanical garden of Samara University (Samara). The research was carried out by the method of light microscopy in transmitted and reflected light in the light background.

Results. Performed analysis allowed to reveal structural characteristics of fruit of Crataegus submollis Sarg. which included cellular structure of the epidermis, lack of stomatal apparatus in epidermis, presence of cortical pores, trichome characteristics, and seed constitution.

Conclusion. Our findings allow to develop the section “Microscopic Signs” of the pharmacopoeial monograph “Fresh Fruit of Crataegus submollis Sarg.”.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):164-170
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Analysis of the problems in the field of medical devices circulation in healthcare organizations
Paygin F.R., Gladunova E.P., Tymoshchuk V.V.
Abstract

The article is devoted to the analysis of violations in the field of circulation of medical devices in healthcare organizations. The results of planned and unscheduled inspections of healthcare organizations of the Samara Region, the findings of sampling of medical devices for control tests, statistical data on the identification of unregistered medical devices with expired shelf life and non-conforming to the established requirements are presented.

Aspirantskiy Vestnik Povolzhiya. 2020;20(1-2):171-178
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