ANTI-AGING EFFECT OF HUMAN PLACENTA HYDROLYSATE. EVIDENCE-BASED STANDARD


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Human placenta hydrolyzates contain biologically active peptides that stimulate the regeneration of damaged tissues. Anti-aging, regenerative and immunomodulating action of Laennek allows to assume that this drug has properties for prolongation of fife. The present article is devoted to the evaluation of the geroprotective action of Laennec using model organism of Caenorhabditis efegans (C. efegans) under the thermal, toxic and oxidative stress conditions. It was shown that under no stress conditions, the addition of Laennec increased the lifespan of C. elegans by 92% compared to the control. Geroprotective effect was observed in a wide range of drug dilutions - from 1: 500 to 1: 312500. Under conditions of thermal stress (t=35°C, 8 h), the survival rate of C. elegans in the presence of Laennec was 20-27% higher compared to the control. Under the conditions of prolonged toxic-oxidative stress (Paraquat 100 mM, 120 h), the addition of Laennec at the optimal dilution 1:62500 increased the average lifespan of C. elegans from 24 to 120 h. Under the conditions of prolonged oxidative stress (H2O2 100 pM, 140 hours), the addition of Laennec increased the lifespan of C. elegans from 22 to 100 h. Thus, Laennec possesses a pronounced geroprotective effect.

Full Text

Restricted Access

About the authors

Sergey V. LEONOV

candidate of biological science, associate professor of the Department of intelligent systems, Moscow institute of physics and technology (state university). 141701, Moscow region, Dolgoprudny, 9 Institutsky pereulok

Yelena I. MARUSICH

candidate of biological sciences, associate professor of the Department of intelligent systems, Moscow institute of physics and technology (state university). 141701, Moscow region, Dolgoprudny, 9 Institutsky pereulok

Olga A. GROMOVA

Email: unesco.gromova@gmail.com
MD, professor of the Department of pharmacology and clinical pharmacology, Ivanovo state medical academy 153012, Ivanovo, 8 Sheremetevsky prospect

Ivan Yu. TORSHIN

candidate of physico-mathematical sciences, associate professor of the Department of intelligent systems, Moscow institute of physics and technology (state university). 141701, Moscow region, Dolgoprudny, 9 Institutsky pereulok

Yekaterina A. DIBROVA

PhD, president of Medical Corporation RHANA 123242, Moscow, 22 Zoologicheskaya St

References

  1. Громова О.А., Торшин И.Ю., Гилельс А.В., Диброва Е.А., Гришина Т.Р., Волков А.Ю., Лиманова О.А., Томилова И.К., Демидов В.И. Препараты плаценты человека: фундаментальные и клинические исследования. Врач. 2014;4:67-72. [Gromova O.A., Torshin I.Yu., Gilel's A.V., Dibrova Ye.A., Grishina T.R., Volkov A.Yu., Limanova O.A., Tomilova I.K., Demidov V.I. Preparations of human placenta: basic science and clinical research. Doctor. 2014;4:67-72 (in Russ.)]
  2. Громова О.А., Марусич Е.И., Торшин И.Ю., Диброва Е.А., Гилельс А.В., Гришина Т.Р., Назаренко О.А., Демидов В.И., Касяник М.С. Кильчевский А.В. Влияние препарата Лаеннек на маркеры старения. Эстетическая медицина. 2017; 1. [Gromova O.A., Marusich Ye.I., Torshin I.Yu., Dibrova Ye.A., Gilel's A.V., Grishina T.R., Nazarenko O.A., Demidov V.l., Kasyanik M.S., Kil'chevsky A.V. Effect of Laennec on the markers of aging. Aesthetic medicine. 2017;1 (in Russ.)]
  3. Ye X., Linton J.M., Schork N.Y., Buck L.B., Petrasheck M.M. A pharmacological network for lifespan extentiom in Caenorhabditis elegans. Aging Cell. 2014;13:206-15.
  4. Biswas T.K., Auddy B., Bhattacharya N.P., Bhattacharya S., Mukherjee B. Wound healing activity of human placental extracts in rats. Acta Pharmacol. Sin. 2001;22(12):1113-6.
  5. Lee K.H., Kim T.H., Lee W.C., Kim S.H., Lee S.Y., Lee S.M. Anti-inflammatory and analgesic effects of human placenta extract. Nat. Prod. Res. 2011;25(1 1):1090-100.
  6. Wu J., Wang C., Liu Q., Yang T., Zhang Q., Peng J., Gao Y., Sun H., Kaku T., Liu K. Protective effect of JBP485 on concanavalin A-induced hepatocyte toxicity in primary cultured rat hepatocytes. Eur. J. Pharmacol. 2008;589(1-3):299-305.
  7. Торшин И.Ю., Згода В.Г., Громова О.А., Баранов И.И., Демидов В.И., Назаренко О.А., Сотникова Н.Ю. Анализ легкой пептидной фракции Лаеннека методами современной протеомики. Фармакокинетика и фармакодинамика. 2016;4:31-42. [Torshin I.Yu., Zgoda V.G., Gromova O.A., Baranov I.I., Demidov V.l., Nazarenko O.A., Sotnikova N.Yu. Analysis of light peptide fractions of Laennec by methods of modern proteomics. Pharmacokinetics and pharmacodynamics. 2016;4:31-42 (in Russ.)]
  8. Song Y., Bi L., Zhang Z., Huang Z., Hou W., Lu X., Sun P., Han Y. Increased levels of calcitonin gene-related peptide in serum accelerate fracture healing following traumatic brain injury. Mol. Med. Report. 2012;5(2):432-8.
  9. Leventoglu S., Koksal H., Sancak B., Taneri F., Onuk E. The effect of calcitonin gene-related peptide on healing of intestinal anastomosis in rats with experimental obstructive jaundice. J. Hepatobiliary Pancreat. Surg. 2009;16(4):546-51.
  10. Анисимов В.Н. Молекулярные и физиологические механизмы старения. 2-е изд., перераб. и доп. СПб.: Наука, 2008. Т. 1, 481 с.; Т. 2, 434 с. [Anisimov V.N. Molecular and physiological mechanisms of aging. 2nd ed. SPb.: Science, 2008. Vol. 1, p. 481; vol. 2, p. 434. (in Russ.)]
  11. Sonntag W.E., Lynch C.D., Cefalu W.T., Ingram R.L., Bennett S.A., Thornton P.L., Khan A.S. Pleiotropic effects of growth hormone and insulin-like growth factor (IGF)-1 on biological aging: inferences from moderate caloric-restricted animals. J. Gerontol. Biol. Sci. 1999;54A:B521-38.
  12. Valenti G. DHEA replacement therapy for human aging: a call for perspective. Aging Clin. Exp. Res. 1997;9(Suppl. 4):71-2.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2017 Bionika Media

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies