The influence of C.189G>A P.(Val66Met) polymorphism of the BDNF gene on serum leptin levels in Yakuts


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Abstract

Numerous studies indicate that BDNF may play an important role in energy homeostasis, in the regulation of food intake, and in the peripheral regulation of metabolism. The aim of the research was to search for the correlation between single nucleotide polymorphism c.189G> A p. (Val66Met) of the BDNF gene and the levels of circulating in blood leptin in Yakut population. Material and methods. Study sample consisted of 281 persons (186 females and 95 males), the average age of the participants was 19,8±1,5 years. The sample was stratified by sex and subdivided according to body mass index (BMI) into three groups: underweight (n=37), normal weight (n=215), and overweight/obesity (n=29) group. Results. In the Yakut population, the frequency of the major allele p. (Val66) was 84,9%, the minor allele p. (Met66) - 15,1%. Due to the low frequency of distribution of homozygotes p. [Met66]; [Met66] and heterozygote p. [Val66]; [Met66], in the analysis, these two genotypes were combined into one group p. [Val66]; [Met66] + p. [Met66]; [Met66]. As a result, an association of the genotypes of p. [Val66]; [Met66] + p. [Met66]; [Met66] with decreased serum leptin levels in normal weight females (p=0,01). In males with normal weight, there was a tendency towards a decrease in leptin level in carriers of the genotypes p. [Val66]; [Met66] + p. [Met66]; [Met66] (3,83±0,85 ng/ml) comparatively with homozygotes p. [Val66]; [Val66] (5,58±0,93 ng/ml; p=0,09). Conclusion. Our results indicate that individuals with p. (Met66) allele probably may have an impaired signaling pathway of leptin through BDNF in the ventromedial hypothalamic nucleus. According to this assumption, carriers of the p. (Met66) allele may have a decreased appetite since their birth time, which leads to a decrease in the amount of consumed foos and an increase in the use of fats in energy metabolism. This, in turn, reduces the accumulation of normal amounts of adipose tissue required for leptin production and is a risk factor for the development of eating disorders associated with anorexia nervosa and bulimia nervosa.

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About the authors

A. A Nikanorova

Yakutsk Scientific Center for Complex Medical Problems

Email: nikanorova.alena@mail.ru
677018, Yakutsk, 6/3 Yaroslavskogo Str

N. A Barashkov

Yakutsk Scientific Center for Complex Medical Problems

Email: barashkov2004@mail.ru
677018, Yakutsk, 6/3 Yaroslavskogo Str

S. S Nakhodkin

M.K. Ammosov North-Eastern University

Email: sergnahod@mail.ru
677010, Yakutsk, 48 Kulakovskogo Str

V. G Pshennikova

Yakutsk Scientific Center for Complex Medical Problems

Email: pshennikovavera@mail.ru
677018, Yakutsk, 6/3 Yaroslavskogo Str

N. N Gotovtsev

Yakutsk Scientific Center for Complex Medical Problems

Email: donzcrew@mail.ru
677018, Yakutsk, 6/3 Yaroslavskogo Str

A. V Solovyev

M.K. Ammosov North-Eastern University

Email: nelloann@mail.ru
677010, Yakutsk, 48 Kulakovskogo Str

G. P Romanov

M.K. Ammosov North-Eastern University

Email: gpromanov@gmail.com
677010, Yakutsk, 48 Kulakovskogo Str

S. S Kuzmina

M.K. Ammosov North-Eastern University

Email: sskuzmina@bk.ru
677010, Yakutsk, 48 Kulakovskogo Str

N. N Sazonov

M.K. Ammosov North-Eastern University

Email: saznikol@mail.ru
677010, Yakutsk, 48 Kulakovskogo Str

S. A Fedorova

M.K. Ammosov North-Eastern University

Email: sardaanafedorova@mail.ru
677010, Yakutsk, 48 Kulakovskogo Str

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