Metal nano- and microparticles-induced oral mucosa immunopathological inflammation
- Authors: Labis V.V.1, Bazikyan E.A.1, Sizova S.V.2, Khaidukov S.V.2, Zhigalina O.M.3, Khmelenin D.N.3, Dyachkova I.G.3, Zolotov D.A.3, Asadchikov V.E.3, Isakina M.O.4, Gildeeva G.N.4, Kozlov I.G.4
-
Affiliations:
- Russian University of Medicine of the Ministry of Healthcare of Russia
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of RAS
- National Research Center “Kurchatov Institute”
- I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University)
- Issue: Vol 10, No 2 (2024)
- Pages: 78-91
- Section: CLINICAL CASE
- URL: https://journals.eco-vector.com/2412-4036/article/view/631833
- DOI: https://doi.org/10.18565/therapy.2024.2.78-91
- ID: 631833
Cite item
Abstract
Studies of the causes and mechanisms of development of oral mucosa chronic inflammation must be carried out taking into account immunological and nanotechnological aspects that fully reflect the picture of cause-effect relations.
The aim: To analyze a clinical case to verify the cause-effect relations in the pathogenesis of idiopathic immunopathological inflammation of oral mucosa induced by metal nano- and microparticles (NMMPs).
Material and methods. In order to verify the main diagnosis in patient Zh., 80 years old, after complex implantological treatment (at the age of 70 years) and the appearance of the first clinical signs of idiopathic immunopathological chronic inflammation of the oral mucosa, clinical and laboratory studies were performed, which made it possible to identify a new etiopathogenetic link of cause-effect relations of arisen complications. During surgical revisions of the area of previously installed dental implants and metal bar structures, histology of biopsy samples of the oral mucosa and bone structures was performed to identify impregnated NMMPs into the structure of pathologically changed tissues. In addition, a basophil test was performed for the presence of delayed-type hypersensitivity with NMMPs-containing supernatants obtained from the surface of the bar structure and dental implants.
Results. The identity of metal NMMPs that impregnated pathological tissue areas with the presence of a chronic inflammatory process and supernatants obtained from the surface of medical devices in laboratory conditions, which were previously installed in the patient’s oral cavity, was found. No delayed allergic reaction to nano-sized particles was fixed.
Conclusion. One of the reasons for oral mucosa chronic autoinflammation occurrence may be the emission and accumulation of metal NMMRs from the surface of medical alloys used in the oral cavity of patients.
Full Text
![Restricted Access](https://journals.eco-vector.com/lib/pkp/templates/images/icons/text_lock.png)
About the authors
Varvara V. Labis
Russian University of Medicine of the Ministry of Healthcare of Russia
Author for correspondence.
Email: varvara2001@mail.ru
ORCID iD: 0000-0001-7632-3234
MD, PhD (Medicine), associate professor of the Department of propaedeutics of surgical dentistry
Russian Federation, 127473, Moscow, 20/1 Delegatskaya St.Ernest A. Bazikyan
Russian University of Medicine of the Ministry of Healthcare of Russia
Email: prof.bazikian@gmail.com
ORCID iD: 0000-0002-9184-3737
MD, Dr. Sci. (Medicine), head of the Department of propaedeutics of surgical dentistry, professor, dean of highly qualified personnel training
Russian Federation, 127473, Moscow, 20/1 Delegatskaya St.Svetlana V. Sizova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of RAS
Email: sv.sizova@gmail.com
ORCID iD: 0000-0003-0846-4670
MD, PhD (Chemistry), researcher at the Department of molecular biophysics
Russian Federation, 117997, Moscow, 16/10 Miklouho-Maklaya St.Sergey V. Khaidukov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of RAS
Email: khsergey54@mail.ru
ORCID iD: 0000-0003-1600-5335
MD, Dr. Sci. (Biology), senior researcher at the Department of carbohydrates
Russian Federation, 117997, Moscow, 16/10 Miklouho-Maklaya St.Olga M. Zhigalina
National Research Center “Kurchatov Institute”
Email: zhigal@crys.ras.ru
ORCID iD: 0000-0003-4721-4105
MD, Dr. Sci. (Physics and Mathematics), leading researcher
Russian Federation, 123182, Moscow, 1 Akademika Kurchatova Sq.Dmitrii N. Khmelenin
National Research Center “Kurchatov Institute”
Email: xorrunn@gmail.com
ORCID iD: 0000-0003-0894-5087
MD, PhD (Physics and Mathematics), researcher
Russian Federation, 123182, Moscow, 1 Akademika Kurchatova Sq.Irina G. Dyachkova
National Research Center “Kurchatov Institute”
Email: dyachkova.i@crys.ras.ru
ORCID iD: 0000-0001-7615-6878
MD, PhD (Physics and Mathematics), senior researcher
Russian Federation, 123182, Moscow, 1 Akademika Kurchatova Sq.Denis A. Zolotov
National Research Center “Kurchatov Institute”
Email: zolotovden@crys.ras.ru
ORCID iD: 0000-0003-3701-9517
MD, PhD (Physics and Mathematics), senior researcher
Russian Federation, 123182, Moscow, 1 Akademika Kurchatova Sq.Victor E. Asadchikov
National Research Center “Kurchatov Institute”
Email: asad@crys.ras.ru
ORCID iD: 0000-0003-3602-7582
MD, Dr. Sci. (Physics and Mathematics), chief researcher
Russian Federation, 123182, Moscow, 1 Akademika Kurchatova Sq.Maria O. Isakina
I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University)
Email: mery.isakina8@mail.ru
ORCID iD: 0009-0008-3764-5941
5th year student of N.F. Filatov Clinical Institute of Child Health
Russian Federation, 119435, Moscow, 19/1 Bol’shaya Pirogovskaya St.Gelia N. Gildeeva
I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University)
Email: gildeeva_g_n@staff.sechenov.ru
ORCID iD: 0000-0002-2537-2850
MD, Dr. Sci. (Medicine), professor, head of the Department of organization and management in the field of drug circulation
Russian Federation, 127018, Moscow, 1/17 Skladochnaya St.Ivan G. Kozlov
I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University)
Email: immunopharmacology@yandex.ru
MD, Dr. Sci. (Medicine), professor, professor of the Department of organization and management in the field of drug circulation
Russian Federation, 127018, Moscow, 1/17 Skladochnaya St.References
- Gao Y., Ye Y., Wang J. et al. Effects of titanium dioxide nanoparticles on nutrient absorption and metabolism in rats: Distinguishing the susceptibility of amino acids, metal elements, and glucose. Nanotoxicology. 2020; 14(10): 1301–23. https://doi.org/10.1080/17435390.2020.1817597. PMID: 32930049.
- Cao X., Han Y., Gu M. et al. Foodborne titanium dioxide nanoparticles induce stronger adverse effects in obese mice than non-obese mice: Gut microbiota dysbiosis, colonic inflammation, and proteome alterations. Small. 2020; 16(36): e2001858. https://doi.org/10.1002/smll.202001858. PMID: 32519440.
- Лабис В.В., Базикян Э.А., Волков А.В. с соавт. Роль иммунных механизмов и оральной микрофлоры в патогенезе периимплантитов. Бюллетень Оренбургского научного центра УрО РАН. 2019; (3): 9. [Labis V.V., Bazikyan E.A., Volkov A.V. et al. Role of immune mechanisms in oral microflora in the pathogenesis of periimplantitis. Byulleten’ Orenburgskogo nauchnogo tsentra Ural’skogo otdeleniya Rossiyskoy akademii nauk = Bulletin of the Orenburg Scientific Center of the Ural Branch of the Russian Academy of Sciences. 2019; (3): 9 (In Russ.)]. EDN: IBZOCU.
- Avila E.D., Oirschot B.A., Beucken J.P. Biomaterial-based possibilities for managing peri-implantitis. J Periodontal Res. 2020; 55(2): 165–73. https://doi.org/10.1111/jre.12707. PMID: 31638267. PMCID: PMC7154698.
- French D., Grandin H.M., Ofec R. Retrospective cohort study of 4,591 dental implants: analysis of risk indicators for bone loss and prevalence of periimplant mucositis and periimplantitis. J Periodontol. 2019; 90(7): 691–700. https://doi.org/10.1002/JPER.18-0236. PMID: 30644101. PMCID: PMC6849729.
- Camacho-Alonso F., Salinas J., Sanchez-Siles M. et al. Synergistic antimicrobial effect of photodynamic therapy and chitosan on the titanium-adherent biofilms of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa: An in vitro study. J Periodontol. 2022; 93(6): e104–15.https://doi.org/10.1002/JPER.21-0306. PMID: 34541685.
- Carvalho E.B.S., Romandini M., Sadilina S. et al. Microbiota associated with peri-implantitis – A systematic review with meta-analyses. Clin Oral Implants Res. 2023; 34(11): 1176–87. https://doi.org/10.1111/clr.14153. PMID: 37523470.
- Labis V., Bazikyan E., Zhigalina O. et al. Assessment of dental implant surface stability at the nanoscale level. Dent Mater. 2022; 38(6): 924–34. https://doi.org/10.1016/j.dental.2022.03.003. PMID: 35289284.
- Базикян Э.А., Лабис В.В., Козлов И.Г. с соавт. Патент № 2611013 С1 Способ персонифицированного подбора дентального имплантата на основе сплавов оксида титана: № 2015152570: заявлен 09.12.2015: опубликован 17.02.2017. [Bazikyan E.A., Labis V.V., Kozlov I.G. et al. Patent No. 2611013 C1 Method for personalized selection of a dental implant based on titanium oxide alloys: No. 2015152570: declared 12/09/2015: published 02/17/2017 (In Russ.)].
- Лабис В.В., Базикян Э.А., Козлов И.Г. с соавт. Микробиологические и иммунологические аспекты взаимодействия Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli с наноразмерными металлическими частицами, полученными с поверхности дентальных имплантатов. Российский иммунологический журнал. 2017; 11(2): 166–169. [Labis V.V., Bazikyan E.A., Kozlov I.G. et al. Microbiological and immunological aspects of the interaction of Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli with nanoscale metal particles, recieved from the surface of dental implants. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology. 2017; 11(2): 166–169 (In Russ.)]. EDN: ZCSCLL.
- Бузмаков А.В., Асадчиков В.Е., Золотов Д.А. с соавт. Лабораторные микротомографы: конструкция и алгоритмы обработки данных. Кристаллография. 2018; 63(6): 1007–1111. [Buzmakov A.V., Asadchikov V.E., Zolotov D.A. et al. Laboratory microtomographs: Design and data processing algorithms. Crystallography Reports. 2018; 63(6): 1007–1111 (In Russ.)]. https://doi.org/10.1134/S0023476118060073. EDN: YMFSMH.
- Румянцев П.О., Козлов И.Г., Колпакова Е.А. с соавт. IGG4-ассоциированные заболевания в эндокринологии. Проблемы эндокринологии. 2020; 66(2): 24–32. [Rumyantsev P.O., Kozlov I.G., Kolpakova E.A. et al. IGG4-related diseases in endocrinology. Problemy endokrinologii = Problems of Endocrinology. 2020; 66(2): 24–32 (In Russ.)]. https://doi.org/10.14341/probl12285. EDN: XSAAEV.
- Hirai T., Yoshioka Y., Izumi N. et al. Metal nanoparticles in the presence of lipopolysaccharides trigger the onset of metal allergy in mice. Nat Nanotechnol. 2016; 11(9): 808–16. https://doi.org/10.1038/nnano.2016.88. PMID: 27240418.
Supplementary files
![](/img/style/loading.gif)