Polymorphism of cytokine genes in patients with liver cirrhosis


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Resumo

Today there is a need to create a set of laboratory tests, including genetic ones, to determine the risk of developing and progression of liver cirrhosis (LC). The aim of the work: to determine the concentration of tumor necrosis factor-a (TNF-a), interleukin 6 (IL-6) and vascular endothelial growth factor (VEGF) in blood serum, as well as their polymorphism genes - TNF-a in the -308G /A region (rs1800629), IL-6 in the -174G/C region (rs1800795) and VEGFA in the -634G/C region (rs2010963) - in LC patients in the Perm region. Material and methods. We examined 46 patients with LC of viral (HCV) and alcoholic etiology and 80 healthy donors. Determined the serum concentration of TNF-a, IL-6 and VEGF by ELISA. The analysis of genetic markers TNF-a -308G/A, IL-6 -174C/G and VEGFA -634G/C by qPCR method. Results. The development of LC is accompanied by activation of inflammatory mechanisms with an increase in the level of proinflammatory cytokines TNF-a (p=0,0001), IL-6 (p=0,0001) and endothelial damage with activation of neoangiogenesis due to hyperproduction of VEGf (p=0,01) in serum. The study of single nucleotide polymorphisms of cytokine genes showed a significantly higher frequency of occurrence in the group of LC patients than in donors of the minor allele A of the TNF-a -308G/A gene, the CG genotype of the IL-6 -174C/G gene and the CC genotype of the VEGFA-634G/C gene. It allows us to attribute them to possible genetic predictors of LC development. Conclusion. Determination of genetic markers TNF-a -308G/A, IL-6 -174C/G and VEGFA-634G/C allows us to assess the increased risk of possible development of LC under the influence of various etiological factors.

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Sobre autores

I. Bulatova

Academician E.A. Vagner Perm State Medical University of the Ministry of Healthcare of Russia

Email: bula.19770mail.ru
614990, Perm, 26 Petropavlovskaya Str

T. Shevlyukova

Tyumen State Medical University of the Ministry of Healthcare of Russia

Email: tata21.010mail.ru
625023, Tyumen, 54 Odesskaya Str

A. Shchekotova

Academician E.A. Vagner Perm State Medical University of the Ministry of Healthcare of Russia

Email: al_shchekotova0mail.ru
614990, Perm, 26 Petropavlovskaya Str

S. Paducheva

City Clinical Hospital No. 2 named after Fedor Khristoforovich Gral

Email: paducheva_sv0mail.ru
614068, Perm, 230 Permskaya Str

A. Martynov

A.I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Healthcare of Russia

Email: anatmartynov0mail.ru
127473, Moscow, 20/1 Delegatskaya Str

Bibliografia

  1. Moon A.M., Singal A.G., Tapper E.B. Contemporary epidemiology of chronic liver disease and cirrhosis. Clin Gastroenterol Hepatol. 2020; 18(12): 2650-66. https://dx.doi.org/10.1016/jxgh.2019.07.060.
  2. GBD 2017 Cirrhosis Collaborators. The global, regional, and national burden of cirrhosis by cause in 195 countries and territories, 1990-2017: A systematic analysis for the Global Burden of Disease Study 2017. Lancet Gastroenterol Hepatol. 2020; 5(3): 245-66. https://dx.doi.org/10.1016/S2468-1253(19)30349-8.
  3. Кузьмина Ю.С., Жмуров В.А., Мищенко Т.А. с соавт. Клинико-лабораторные факторы неблагоприятного прогноза заболевания у пациентов с циррозом печени. Медицинская наука и образование Урала. 2020; 3: 13-16. @@Kuzmina Yu.S., Zhmurov V.A., Mishchenko T.A. et al. Clinical and laboratory factors of unfavorable prognosis of the disease in patients with cirrhosis of the liver Meditsinskaya nauka i obrazovaniye Urala = Medical Science and Education of the Urals. 2020; 3: 13-16 (In Russ.). https://dx.doi.org/10.36361/1814-8999-2020-21-3-12-16.
  4. Brenner D.A. Molecular pathogenesis of liver fibrosis. Trans Am Clin Climatol Assoc. 2009; 120: 361-68.
  5. Долгих О.В., Падучева С.В., Булатова И.А., Щекотова А.П. Особенности иммунологического ответа при циррозе печени в зависимости от степени его тяжести. Российский иммунологический журнал. 2017; 3: 463-465. @@Paducheva S.V., Dolgikh O.V., Bulatova I.A., Shekotova A.P. Peculiarities of immunological responce during liver cirrhosis depending on severity level. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology. 2017; 3: 463-465 (In Russ.)
  6. Булатова И.А. Васкулоэндотелиальный фактор роста и полиморфизм гена VEGF (rs2010963) в патогенезе хронического вирусного гепатита С. Современные проблемы науки и образования. 2013; 6: 635. @@Bulatova I.A. Vascular endothelial grow factor and polymorphism of VEGF (RS2010963) gene in pathogenesis of chronic hepatitis C. Sovremennye problemy nauki i obrazovaniia = Modern Issues of Science and Education. 2013; 6: 635 (In Russ.)
  7. Щекотов В.В., Булатова И.А., Щекотова А.П. с соавт. Неинвазивная оценка темпа прогрессирования фиброза печени. Современные проблемы науки и образования. 2016; 1: 5. @@Schekotov V.V., Bulatova I.A., Schekotova A.P. et al. Noninvasive evaluation of progress of liver fibrosis. Sovremennye problemy nauki i obrazovaniia = Modern Issues of Science and Education. 2016; 1: 5 (In Russ.)
  8. Huang H., Shiffman M.L., Friedman S. et al. A 7 gene signature identifies the risk of developing cirrhosis in patients with chronic hepatitis C. Hepatology. 2007; 46(2): 297-306. https://dx.doi.org/10.1002/hep.21695.
  9. Guinot F., Szafranski M., Ambroise C. et al. Learning the optimal scale for GWAS through hierarchical SNP aggregation. BMC Bioinformatics. 2018; 19(1): 459. https://dx.doi.org/10.1186/s12859-018-2475-9.
  10. Бодиенкова Г.М., Титова Ж.В. Роль полиморфизма и экспрессии отдельных генов цитокинов в формировании патологии (обзор). Успехи современного естествознания. 2015; 1-4: 616-620. @@Titova Z.V., Bodienkova G.M. Polymorphism and gene expression cytokines in the formation of pathology (review). Uspekhi sovremennogo yestestvoznaniya = Successes of Modern Natural Science. 2015; 1-4: 616-620 (In Russ.)
  11. EASL Clinical Practice Guidelines: Management of alcohol-related liver disease. European Association for the Study of the Liver. J Hepatol. 2018; 69(1): 154-81. https://dx.doi.org/10.1016/j.jhep.2018.03.018.
  12. Albillos A., Lario М., Alvarez-Mon М. Cirrosis-associated immune distinctive features and clinical relevance. J Hepatol 2014; 61(6): 1385-96. https://dx.doi.org/10.1016/j.jhep.2014.08.010.
  13. Dirchwolf М., Podhorzer А., Morino М. et al. Immune dysfunction in cirrhosis: Distinct cytokines phenotypes according to cirrhosis severity. J Cytokine. 2016; 77: 14-25. https://dx.doi.org/10.1016/j.cyto.2015.10.006.
  14. Martinez-Esparza M., Tristan-Manzano М., Ruiz-Alcaraz A.J., Garda-Penarrubia Р. Inflammatory status in human hepatic cirrhosis. World J Gastroenterol. 2015; 21(41): 11522-41. https://dx.doi.org/10.3748/wjg.v21.i41.11522.
  15. Скуратов А.Г, Лызиков А.Н., Воропаев Е.В., Осипкина О.В. Уровень интерлейкина-6 как показатель тяжести цирроза печени и портальной гипертензии. Проблемы здоровья и экологии. 2016; 4: 110-114. @@Skuratov A.G., Lyzikov A.N., Voropaev E.V., Osipkina O.V.Interleukin-6 as an indicator of severity of liver cirrhosis and portal hypertension. Problemy zdorov'ya i ekologii = Issues of Health and Ecology. 2016; 4: 110-114 (In Russ.)
  16. Николаева Л.И., Колотвин А.В., Самоходская Л.М. с соавт. Анализ влияния генетических факторов вируса гепатита С и полиморфизма генов инфицированных людей на развитие фиброза печени. Эпидемиология и инфекционные болезни. 2012; 5: 7-13. @@Nikolaeva L.I., Kolotvin A.V., Samokhodskaya L.M. et al. Analysis of the influence of genetic factors of hepatitis C virus and gene polymorphism in infected patients on the development of liver fibrosis. Epidemiologiya i infektsionnyye bolezni = Epidemiology and Infectious Diseases. 2012; 5: 7-13 (In Russ.)
  17. Скуратов А.Г, Лызиков А.Н., Воропаев Е.В., Осипкина О.В. Молекулярно-генетический анализ полиморфизма генов IL-4, TNF-a у пациентов с циррозом печени и портальной гипертензией. Проблемы здоровья и экологии. 2017; 2: 110-114. @@Skuratov A.G., Lyzikov A.N., Voropayev E.V., Osipkina O.V. The molecular-genetic analysis of polymorphism of IL-4, TNF-a genes in patients with liver cirrhosis and portal hypertension. Problemy zdorov'ya i ekologii = Issues of Health and Ecology. 2017; 2: 110-114 (In Russ.)
  18. Giannitrapani L., Soresi M., Balasus D. et al. Genetic association of interleukin-6 polymorphism (-174 G/C) with chronic liver diseases and hepatocellular carcinoma. World J Gastroenterol. 2013; 19(16): 2449-55. https://dx.doi.org/10.3748/wjg.v19.i16.2449.

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