The effect of the composition of hepatoprotective action on biochemical and morphostructural changes in the body of laying hens under thermal stress

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To reduce the negative effects of hyperthermia on the body of farm animals and poultry, various drugs and feed additives are currently used. Which do not have sufficiently adaptogenic and antitoxic properties. We studied the effect of a hepatoprotective composition consisting of dried live yeast, amorphous silicon dioxide, propylene glycol, calcium propionate, ascorbic acid, manganese, copper and zinc chelates, methionine and choline chloride on the variability of biochemical and morphological parameters of the body of laying hens under temperature stress. It was simulated by increasing the air temperature in a building where laying hens were kept from 18.0 ± 1.0 °C to 28.0 ± 1.0 °C for 48 hours. Due to hyperthermia, changes in biochemical and morphofunctional parameters were observed in the tissues and organs of birds. The obtained values of the biochemical parameters of blood serum in the birds of the control group indicated the intensity of the adaptive capabilities of their body. A complex of morphological changes confirmed a violation of protein metabolism and the regenerative-compensatory process. Pathological changes in the structure of the duodenum, characteristic of catarrhal-necrotic duodenitis, were identified. The stress reaction was also reflected in the condition of the heart muscle, in which an inflammatory process developed against the background of granular dystrophy of cardiomyocytes. The results of biochemical studies of blood serum in birds of the experimental group indicated an increase in the anti-stress response to a temperature stimulus under the influence of the studied composition (tendency to increase glucose and calcium, increase alkaline phosphatase activity by 47.4 %). The introduction of a hepatoprotective composition into the diet of laying hens during periods of temperature stress did not lead to disruption of the structure of tissues and organs, preserving cellular metabolic mechanisms. The

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作者简介

L. Drozdova

Ural Federal Agrarian Scientific Research Center, Ural Branch of the Russian Academy of Sciences

编辑信件的主要联系方式.
Email: marafon.86@list.ru

доктор ветеринарных наук

俄罗斯联邦, 620142, Ekaterinburg, ul. Belinskogo, 112a

A. Krasnoperov

Ural Federal Agrarian Scientific Research Center, Ural Branch of the Russian Academy of Sciences

Email: marafon.86@list.ru

кандидат ветеринарных наук

俄罗斯联邦, 620142, Ekaterinburg, ul. Belinskogo, 112a

O. Oparina

Ural Federal Agrarian Scientific Research Center, Ural Branch of the Russian Academy of Sciences

Email: marafon.86@list.ru

кандидат ветеринарных наук

俄罗斯联邦, 620142, Ekaterinburg, ul. Belinskogo, 112a

S. Malkov

Ural Federal Agrarian Scientific Research Center, Ural Branch of the Russian Academy of Sciences

Email: marafon.86@list.ru

кандидат ветеринарных наук

俄罗斯联邦, 620142, Ekaterinburg, ul. Belinskogo, 112a

A. Belousov

Ural Federal Agrarian Scientific Research Center, Ural Branch of the Russian Academy of Sciences

Email: marafon.86@list.ru

доктор ветеринарных наук

俄罗斯联邦, 620142, Ekaterinburg, ul. Belinskogo, 112a

A. Chernitskiy

Ural Federal Agrarian Scientific Research Center, Ural Branch of the Russian Academy of Sciences

Email: marafon.86@list.ru

доктор биологических наук

俄罗斯联邦, 620142, Ekaterinburg, ul. Belinskogo, 112a

参考

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2. Fig. 1. Histological structure of organs (duodenum, pancreas) of laying hens. Hematoxylin and eosin staining (photo by L. I. Drozdova): a – pseudoeosinophilia in the interstitial tissue of the glandular apparatus of the duodenum; b – mucus hypersecretion, mucous catarrhal duodenitis; c – catarrhal-necrotic duodenitis, necrosis and desquamation of the layers of the epithelium of the villous tissue; g – oozing of mucous exudate into the lumen of the duodenum; d – islet of Langerhans of the pancreas, decreased secretion; e – hypersecretion and hyperplasia of the islets of Langerhans.

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3. Fig. 2. Histological structure of organs (liver, heart muscle) of laying hens, stained with hematoxylin and eosin (photo by Drozdova L. I.): a – polymorphic cell perivascular infiltration in the triad system against the background of granular dystrophy of hepatocytes; b – granular dystrophy of hepatocytes, edema of the interstitial tissue in the triad system; c – signs of myocarditis in the heart muscle; d – perivascular edema and proliferation of vascular wall elements, granular dystrophy of cardiomyocytes.

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4. Fig. 3. Histological structure of organs (kidneys, spleen) of laying hens. Hematoxylin and eosin staining (photo by Drozdova L. I.): a – glomerular amyloidosis, granular dystrophy of nephrocytes; b – necrosis of the epithelium of the convoluted tubules of the kidney, glomerular amyloidosis; c – glomerulitis and protein metabolism disorder in nephrocytes; g – splenic hyperplasia; e – foci of hyperplasia in the pulp of the spleen.

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5. Fig. 4. Histological structure of organs (thymus) of laying hens. Hematoxylin and eosin staining (photo by Drozdova L.I.): a – phagocytosis of necrotic Hassall’s corpuscle by pseudoeosinophils; b – involution of Hassall’s corpuscles.

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